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Bone dynamics in the upward direction after a maxillary sinus floor elevation procedure: serial segmentation using synchrotron radiation micro-computed tomography.

Seo SJ, Bark CW, Lim JH, Kim YG - Int J Nanomedicine (2015)

Bottom Line: The bone dynamics between the new bone and bone substitutes along the inferior-superior direction in humans after maxillary sinus floor elevation (MSFE) were analyzed using the whole sample region.SR-μCT is highly effective for obtaining high-resolution images.An analysis of biological specimens using SR-μCT is quite reliable and this technique will be an important tool in the wide field of tissue engineering.

View Article: PubMed Central - PubMed

Affiliation: Industrial Technology Convergence Center, Pohang Accelerator Laboratory, POSTECH, Pohang, Gyeongbuk, Republic of Korea.

ABSTRACT

Objective: Maxillary sinus floor augmentation has been shown to be the most predictable surgical technique for enhancing the bone volume in the posterior area of the maxilla. The purpose of this study was to analyze the serial slice image segmentation of newly formed bone and bone substitutes after sinus floor elevation using synchrotron radiation X-ray micro-computed tomography (SR-μCT).

Materials and methods: Bone biopsy specimens were collected after 6 months of sinus floor augmentation. From the six bone biopsy specimens, the cross-sectional images at every 8 μm along the apical direction from the inferior border using serial segmentation from three-dimensional reconstructed X-ray images were analyzed. The amount of new bone and bone substitutes were measured at each slicing image (300-430 images per specimen).

Results: The bone dynamics between the new bone and bone substitutes along the inferior-superior direction in humans after maxillary sinus floor elevation (MSFE) were analyzed using the whole sample region. Although these observations suggest that the specimens are structurally inhomogeneous, sinus floor elevation was confirmed to be a reliable surgical procedure for increasing the amount of bone.

Conclusion: SR-μCT is highly effective for obtaining high-resolution images. An analysis of biological specimens using SR-μCT is quite reliable and this technique will be an important tool in the wide field of tissue engineering.

No MeSH data available.


Related in: MedlinePlus

Schematic diagrams of the SR-μCT experiment performed at the 6C BMI beamline at PLS-II.Notes: A 23 keV monochromatic beam was attenuated by a graphite attenuator and the beam propagated 15 cm from sample to a YAG:Ce 50 μm. The images stored in a CCD camera were transferred to a PC for further data processing. Morphometric analysis by SR-μCT was performed using a reconstruction of the 2D cross sections of the sample or 3D image visualization of the sample.Abbreviations: BMI, biomedical imaging; CCD, charge-coupled device; CT, computed tomography; 2D, two-dimensional; 3D, three-dimensional; GPU, graphics processing unit; PLS-II, pohang light source II; SR-μCT, synchrotron radiation X-ray micro-computed tomography; PC, personal computer.
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f2-ijn-10-129: Schematic diagrams of the SR-μCT experiment performed at the 6C BMI beamline at PLS-II.Notes: A 23 keV monochromatic beam was attenuated by a graphite attenuator and the beam propagated 15 cm from sample to a YAG:Ce 50 μm. The images stored in a CCD camera were transferred to a PC for further data processing. Morphometric analysis by SR-μCT was performed using a reconstruction of the 2D cross sections of the sample or 3D image visualization of the sample.Abbreviations: BMI, biomedical imaging; CCD, charge-coupled device; CT, computed tomography; 2D, two-dimensional; 3D, three-dimensional; GPU, graphics processing unit; PLS-II, pohang light source II; SR-μCT, synchrotron radiation X-ray micro-computed tomography; PC, personal computer.

Mentions: After 6 months of uneventful healing, the implant sites were prepared with trephine burs (3.0 mm outer diameter, 2.0 mm inner diameter) to obtain bone cores from the implant sites (vertical biopsies). In each patient, one biopsy was harvested at one of the sites, where a dental implant was to be placed (Table 1). The bone specimens were fixed in 4% neutral-buffered paraformaldehyde and used for the conventional histological, histomorphometric, and SR-μCT investigations. The specimen contained both a grafted area and a residual alveolar process. The residual native bone was included in the histological examination, but not in the histomorphometric and SR-μCT analyses. In the analysis, the interested volume and area were approximately 3 mm (height) ×2 mm (diameter) for SR-μCT and approximately 3 mm (height) ×2 mm (width) for histomorphometric analysis in the grafted area at each of the six samples. The upper part of Figure 2 presents a schematic diagram of the experimental procedure.


Bone dynamics in the upward direction after a maxillary sinus floor elevation procedure: serial segmentation using synchrotron radiation micro-computed tomography.

Seo SJ, Bark CW, Lim JH, Kim YG - Int J Nanomedicine (2015)

Schematic diagrams of the SR-μCT experiment performed at the 6C BMI beamline at PLS-II.Notes: A 23 keV monochromatic beam was attenuated by a graphite attenuator and the beam propagated 15 cm from sample to a YAG:Ce 50 μm. The images stored in a CCD camera were transferred to a PC for further data processing. Morphometric analysis by SR-μCT was performed using a reconstruction of the 2D cross sections of the sample or 3D image visualization of the sample.Abbreviations: BMI, biomedical imaging; CCD, charge-coupled device; CT, computed tomography; 2D, two-dimensional; 3D, three-dimensional; GPU, graphics processing unit; PLS-II, pohang light source II; SR-μCT, synchrotron radiation X-ray micro-computed tomography; PC, personal computer.
© Copyright Policy
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4554393&req=5

f2-ijn-10-129: Schematic diagrams of the SR-μCT experiment performed at the 6C BMI beamline at PLS-II.Notes: A 23 keV monochromatic beam was attenuated by a graphite attenuator and the beam propagated 15 cm from sample to a YAG:Ce 50 μm. The images stored in a CCD camera were transferred to a PC for further data processing. Morphometric analysis by SR-μCT was performed using a reconstruction of the 2D cross sections of the sample or 3D image visualization of the sample.Abbreviations: BMI, biomedical imaging; CCD, charge-coupled device; CT, computed tomography; 2D, two-dimensional; 3D, three-dimensional; GPU, graphics processing unit; PLS-II, pohang light source II; SR-μCT, synchrotron radiation X-ray micro-computed tomography; PC, personal computer.
Mentions: After 6 months of uneventful healing, the implant sites were prepared with trephine burs (3.0 mm outer diameter, 2.0 mm inner diameter) to obtain bone cores from the implant sites (vertical biopsies). In each patient, one biopsy was harvested at one of the sites, where a dental implant was to be placed (Table 1). The bone specimens were fixed in 4% neutral-buffered paraformaldehyde and used for the conventional histological, histomorphometric, and SR-μCT investigations. The specimen contained both a grafted area and a residual alveolar process. The residual native bone was included in the histological examination, but not in the histomorphometric and SR-μCT analyses. In the analysis, the interested volume and area were approximately 3 mm (height) ×2 mm (diameter) for SR-μCT and approximately 3 mm (height) ×2 mm (width) for histomorphometric analysis in the grafted area at each of the six samples. The upper part of Figure 2 presents a schematic diagram of the experimental procedure.

Bottom Line: The bone dynamics between the new bone and bone substitutes along the inferior-superior direction in humans after maxillary sinus floor elevation (MSFE) were analyzed using the whole sample region.SR-μCT is highly effective for obtaining high-resolution images.An analysis of biological specimens using SR-μCT is quite reliable and this technique will be an important tool in the wide field of tissue engineering.

View Article: PubMed Central - PubMed

Affiliation: Industrial Technology Convergence Center, Pohang Accelerator Laboratory, POSTECH, Pohang, Gyeongbuk, Republic of Korea.

ABSTRACT

Objective: Maxillary sinus floor augmentation has been shown to be the most predictable surgical technique for enhancing the bone volume in the posterior area of the maxilla. The purpose of this study was to analyze the serial slice image segmentation of newly formed bone and bone substitutes after sinus floor elevation using synchrotron radiation X-ray micro-computed tomography (SR-μCT).

Materials and methods: Bone biopsy specimens were collected after 6 months of sinus floor augmentation. From the six bone biopsy specimens, the cross-sectional images at every 8 μm along the apical direction from the inferior border using serial segmentation from three-dimensional reconstructed X-ray images were analyzed. The amount of new bone and bone substitutes were measured at each slicing image (300-430 images per specimen).

Results: The bone dynamics between the new bone and bone substitutes along the inferior-superior direction in humans after maxillary sinus floor elevation (MSFE) were analyzed using the whole sample region. Although these observations suggest that the specimens are structurally inhomogeneous, sinus floor elevation was confirmed to be a reliable surgical procedure for increasing the amount of bone.

Conclusion: SR-μCT is highly effective for obtaining high-resolution images. An analysis of biological specimens using SR-μCT is quite reliable and this technique will be an important tool in the wide field of tissue engineering.

No MeSH data available.


Related in: MedlinePlus