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Histochemical analysis of paraspinal rotator muscles from patients with adolescent idiopathic scoliosis: a cross-sectional study.

Wajchenberg M, Martins DE, Luciano Rde P, Puertas EB, Del Curto D, Schmidt B, Oliveira AB, Faloppa F - Medicine (Baltimore) (2015)

Bottom Line: Results showed significant differences in both endomysial and perimysial fibrosis and fatty involution between the two sides of the apex vertebra.Paraspinal muscles in the concave side of the scoliosis apex had significantly more fibrosis and fatty involution.However, both sides showed signs of myopathy, muscular atrophy due to necrosis, presence of hyaline fibers, and mitochondrial proliferation.

View Article: PubMed Central - PubMed

Affiliation: From the Universidade Federal de Sao Paulo (UNIFESP/EPM), Rua Borges Lagoa, Vila Clementino, Sao Paulo, Brazil.

ABSTRACT
Morphological, biochemical, and histopathological alterations in the paraspinal skeletal muscle of patients with adolescent idiopathic scoliosis (AIS) have been extensively reported. We evaluated rotator muscle fibers from the apex vertebra of AIS patients through histological and immunohistochemical analysis. A population of 21 female AIS patients who underwent corrective surgery between 2010 and 2013 had biopsies taken from the paraspinal muscle in the convex and concave sides of the thoracic curve apical vertebra. Serial sections were stained following routine protocols for hematoxylin and eosin (HE), Sudan red, Gomori trichrome, NADH, ATPase, and cytochrome oxidase. We assessed muscular atrophy and hypertrophy, fatty proliferation, endomysial and perimysial fibrosis, the presence of hyaline fibers, mitochondrial proliferation, muscular necrosis, nuclear centralization, and inflammation. Two independent professionals evaluated the slices. The thoracic curves had an average Cobb angle of 68 degree. Comparative analysis of the concave and convex sides was performed with McNemar test at a significance level of 5%. Results showed significant differences in both endomysial and perimysial fibrosis and fatty involution between the two sides of the apex vertebra. Paraspinal muscles in the concave side of the scoliosis apex had significantly more fibrosis and fatty involution. However, both sides showed signs of myopathy, muscular atrophy due to necrosis, presence of hyaline fibers, and mitochondrial proliferation.

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NADH stain of rotator muscle tissue from an adolescent idiopathic scoliosis patient showing central core lesion of the muscle fiber.
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Figure 4: NADH stain of rotator muscle tissue from an adolescent idiopathic scoliosis patient showing central core lesion of the muscle fiber.

Mentions: Comparative analysis of the concave and convex sides of each sample was performed with McNemar test at a significance level of 5%. Relative frequencies found and P values are presented in Table 2. Endomysial and perimysial fibrosis as well as fatty involution were significantly greater in the concave side of the apex vertebra (Table 2 and Figure 1). We were also able to identify other alterations, albeit with no significant differences between curvature sides. These included hyaline fibers, muscle necrosis, and nuclear centralization (Figure 2); inflammation, muscular atrophy, and mitochondrial proliferation (Figure 3); and low-oxidative areas in the muscle fibers suggestive of central core myopathy (Figure 4).


Histochemical analysis of paraspinal rotator muscles from patients with adolescent idiopathic scoliosis: a cross-sectional study.

Wajchenberg M, Martins DE, Luciano Rde P, Puertas EB, Del Curto D, Schmidt B, Oliveira AB, Faloppa F - Medicine (Baltimore) (2015)

NADH stain of rotator muscle tissue from an adolescent idiopathic scoliosis patient showing central core lesion of the muscle fiber.
© Copyright Policy - open-access
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4554143&req=5

Figure 4: NADH stain of rotator muscle tissue from an adolescent idiopathic scoliosis patient showing central core lesion of the muscle fiber.
Mentions: Comparative analysis of the concave and convex sides of each sample was performed with McNemar test at a significance level of 5%. Relative frequencies found and P values are presented in Table 2. Endomysial and perimysial fibrosis as well as fatty involution were significantly greater in the concave side of the apex vertebra (Table 2 and Figure 1). We were also able to identify other alterations, albeit with no significant differences between curvature sides. These included hyaline fibers, muscle necrosis, and nuclear centralization (Figure 2); inflammation, muscular atrophy, and mitochondrial proliferation (Figure 3); and low-oxidative areas in the muscle fibers suggestive of central core myopathy (Figure 4).

Bottom Line: Results showed significant differences in both endomysial and perimysial fibrosis and fatty involution between the two sides of the apex vertebra.Paraspinal muscles in the concave side of the scoliosis apex had significantly more fibrosis and fatty involution.However, both sides showed signs of myopathy, muscular atrophy due to necrosis, presence of hyaline fibers, and mitochondrial proliferation.

View Article: PubMed Central - PubMed

Affiliation: From the Universidade Federal de Sao Paulo (UNIFESP/EPM), Rua Borges Lagoa, Vila Clementino, Sao Paulo, Brazil.

ABSTRACT
Morphological, biochemical, and histopathological alterations in the paraspinal skeletal muscle of patients with adolescent idiopathic scoliosis (AIS) have been extensively reported. We evaluated rotator muscle fibers from the apex vertebra of AIS patients through histological and immunohistochemical analysis. A population of 21 female AIS patients who underwent corrective surgery between 2010 and 2013 had biopsies taken from the paraspinal muscle in the convex and concave sides of the thoracic curve apical vertebra. Serial sections were stained following routine protocols for hematoxylin and eosin (HE), Sudan red, Gomori trichrome, NADH, ATPase, and cytochrome oxidase. We assessed muscular atrophy and hypertrophy, fatty proliferation, endomysial and perimysial fibrosis, the presence of hyaline fibers, mitochondrial proliferation, muscular necrosis, nuclear centralization, and inflammation. Two independent professionals evaluated the slices. The thoracic curves had an average Cobb angle of 68 degree. Comparative analysis of the concave and convex sides was performed with McNemar test at a significance level of 5%. Results showed significant differences in both endomysial and perimysial fibrosis and fatty involution between the two sides of the apex vertebra. Paraspinal muscles in the concave side of the scoliosis apex had significantly more fibrosis and fatty involution. However, both sides showed signs of myopathy, muscular atrophy due to necrosis, presence of hyaline fibers, and mitochondrial proliferation.

Show MeSH
Related in: MedlinePlus