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Synchronous Resectable Metastatic Colorectal Cancer: Lymph Node Involvement Predicts Poor Outcome.

Fu J, Jiang M, Tan Y, Yang J, Wu L, Feng L, Zheng S, Yuan Y - Medicine (Baltimore) (2015)

Bottom Line: In the COX model, the 7th AJCC TNM N-stage and LNR were independent prognostic factors.The mRNA profile was not associated with lymph node involvement.Both the N-stage according to the 7th AJCC TNM staging system and LNR had the capacity to subclassify synchronous resectable mCRC with different prognoses.The lymph node might be integrated into the AJCC staging system as a diagnose-delay prognostic factor for stage IV disease.

View Article: PubMed Central - PubMed

Affiliation: From the Department of Medical Oncology, The Second Affiliated Hospital, Zhejiang University School of Medicine, Hangzhou (JF, MJ, YT, JY, YY); Department of Oncology, Jinhua Central Hospital (Jinhua Hospital of Zhejiang University School of Medicine), Jinhua (JF); Cancer Institute (Key Laboratory of Cancer Prevention and Intervention, China National Ministry of Education, Key Laboratory of Molecular Biology in Medical Sciences) (JF, MJ, YT, JY, SZ, YY); Department of Gastroenterology, The Second Affiliated Hospital, Zhejiang University School of Medicine, Hangzhou, Zhejiang (LW); and State Key Laboratory of Molecular Oncology, Department of Aetiology and Carcinogenesis, Cancer Institute and Hospital, Peking Union Medical College and Chinese Academy of Medical Sciences, Beijing, China (LF).

ABSTRACT
To evaluate the value of lymph node status of primary tumors in predicting the prognosis of synchronous resectable metastatic colorectal cancer (mCRC).The characteristics of resectable mCRC are substantially different from other cancers, and the prognostic factors of resectable mCRC are still controversial.The data of 2007 patients with mCRC who received resection of the primary tumors and metastatic lesions synchronously were reviewed from the Surveillance, Epidemiology and End-Result database. The Kaplan-Meier method was used to evaluate the capacity of different prognostic factors. Univariate and multivariate logistic regression models were used to evaluate the relationship between the lymph node status and other factors. The mRNA profiles of primary resectable mCRC tumors were obtained by microarray at our center.The median survival times were 50, 36, 32, 27, and 19 months in the N0-stage, N1a-stage, N1b-stage, N2a-stage, and N2b-stage subgroups according to the 7th American Joint Committee on Cancer (AJCC) Tumor Lymph Node Metastasis (TNM) N-classification (P = 0.000), and 40, 29, 22, and 15 months in patients with metastatic lymph node ratio (LNR) <0.25, 0.25-0.49, 0.5-0.74, and ≥0.75 subgroups (P = 0.000). In the COX model, the 7th AJCC TNM N-stage and LNR were independent prognostic factors. The mRNA profile was not associated with lymph node involvement.Both the N-stage according to the 7th AJCC TNM staging system and LNR had the capacity to subclassify synchronous resectable mCRC with different prognoses. The lymph node might be integrated into the AJCC staging system as a diagnose-delay prognostic factor for stage IV disease.

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Clustering analysis of 15 samples from primary tumors of resectable metastatic colorectal cancers representing 8 patients with positive lymph node and 7 patients with negative lymph node. mRNAs were included in the tree when normalized expression values ranged from 20% to 70% of all mRNAs. A total of 1240 mRNAs were retained for clustering. Hierarchical clustering with average linkage using Pearson correlation as the distance metrics was applied to cluster the samples according to their mRNA-normalized expression values.
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Figure 4: Clustering analysis of 15 samples from primary tumors of resectable metastatic colorectal cancers representing 8 patients with positive lymph node and 7 patients with negative lymph node. mRNAs were included in the tree when normalized expression values ranged from 20% to 70% of all mRNAs. A total of 1240 mRNAs were retained for clustering. Hierarchical clustering with average linkage using Pearson correlation as the distance metrics was applied to cluster the samples according to their mRNA-normalized expression values.

Mentions: The detailed characteristics of 15 patients with resectable mCRC selected in our center are provided in Appendix 2, http://links.lww.com/MD/A347. The mRNA profiles of the primary tumor tissues were analyzed. No obvious difference was found between the lymph node positive subgroup and the lymph node negative subgroup based on the heat maps of mRNA profiles (Figure 4). There was no distinct mRNA expression profile identified among 27,598 eligible entities.


Synchronous Resectable Metastatic Colorectal Cancer: Lymph Node Involvement Predicts Poor Outcome.

Fu J, Jiang M, Tan Y, Yang J, Wu L, Feng L, Zheng S, Yuan Y - Medicine (Baltimore) (2015)

Clustering analysis of 15 samples from primary tumors of resectable metastatic colorectal cancers representing 8 patients with positive lymph node and 7 patients with negative lymph node. mRNAs were included in the tree when normalized expression values ranged from 20% to 70% of all mRNAs. A total of 1240 mRNAs were retained for clustering. Hierarchical clustering with average linkage using Pearson correlation as the distance metrics was applied to cluster the samples according to their mRNA-normalized expression values.
© Copyright Policy - open-access
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4554134&req=5

Figure 4: Clustering analysis of 15 samples from primary tumors of resectable metastatic colorectal cancers representing 8 patients with positive lymph node and 7 patients with negative lymph node. mRNAs were included in the tree when normalized expression values ranged from 20% to 70% of all mRNAs. A total of 1240 mRNAs were retained for clustering. Hierarchical clustering with average linkage using Pearson correlation as the distance metrics was applied to cluster the samples according to their mRNA-normalized expression values.
Mentions: The detailed characteristics of 15 patients with resectable mCRC selected in our center are provided in Appendix 2, http://links.lww.com/MD/A347. The mRNA profiles of the primary tumor tissues were analyzed. No obvious difference was found between the lymph node positive subgroup and the lymph node negative subgroup based on the heat maps of mRNA profiles (Figure 4). There was no distinct mRNA expression profile identified among 27,598 eligible entities.

Bottom Line: In the COX model, the 7th AJCC TNM N-stage and LNR were independent prognostic factors.The mRNA profile was not associated with lymph node involvement.Both the N-stage according to the 7th AJCC TNM staging system and LNR had the capacity to subclassify synchronous resectable mCRC with different prognoses.The lymph node might be integrated into the AJCC staging system as a diagnose-delay prognostic factor for stage IV disease.

View Article: PubMed Central - PubMed

Affiliation: From the Department of Medical Oncology, The Second Affiliated Hospital, Zhejiang University School of Medicine, Hangzhou (JF, MJ, YT, JY, YY); Department of Oncology, Jinhua Central Hospital (Jinhua Hospital of Zhejiang University School of Medicine), Jinhua (JF); Cancer Institute (Key Laboratory of Cancer Prevention and Intervention, China National Ministry of Education, Key Laboratory of Molecular Biology in Medical Sciences) (JF, MJ, YT, JY, SZ, YY); Department of Gastroenterology, The Second Affiliated Hospital, Zhejiang University School of Medicine, Hangzhou, Zhejiang (LW); and State Key Laboratory of Molecular Oncology, Department of Aetiology and Carcinogenesis, Cancer Institute and Hospital, Peking Union Medical College and Chinese Academy of Medical Sciences, Beijing, China (LF).

ABSTRACT
To evaluate the value of lymph node status of primary tumors in predicting the prognosis of synchronous resectable metastatic colorectal cancer (mCRC).The characteristics of resectable mCRC are substantially different from other cancers, and the prognostic factors of resectable mCRC are still controversial.The data of 2007 patients with mCRC who received resection of the primary tumors and metastatic lesions synchronously were reviewed from the Surveillance, Epidemiology and End-Result database. The Kaplan-Meier method was used to evaluate the capacity of different prognostic factors. Univariate and multivariate logistic regression models were used to evaluate the relationship between the lymph node status and other factors. The mRNA profiles of primary resectable mCRC tumors were obtained by microarray at our center.The median survival times were 50, 36, 32, 27, and 19 months in the N0-stage, N1a-stage, N1b-stage, N2a-stage, and N2b-stage subgroups according to the 7th American Joint Committee on Cancer (AJCC) Tumor Lymph Node Metastasis (TNM) N-classification (P = 0.000), and 40, 29, 22, and 15 months in patients with metastatic lymph node ratio (LNR) <0.25, 0.25-0.49, 0.5-0.74, and ≥0.75 subgroups (P = 0.000). In the COX model, the 7th AJCC TNM N-stage and LNR were independent prognostic factors. The mRNA profile was not associated with lymph node involvement.Both the N-stage according to the 7th AJCC TNM staging system and LNR had the capacity to subclassify synchronous resectable mCRC with different prognoses. The lymph node might be integrated into the AJCC staging system as a diagnose-delay prognostic factor for stage IV disease.

Show MeSH
Related in: MedlinePlus