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Cytokine profiles contribute to understanding the pathogenic difference between Good syndrome and oral lichen planus: two case reports and literature review.

Maehara T, Moriyama M, Kawano S, Hayashida JN, Furukawa S, Ohta M, Tanaka A, Yamauchi M, Ohyama Y, Kiyoshima T, Nakamura S - Medicine (Baltimore) (2015)

Bottom Line: However, IL-4 and IL-17 were detected in OLP patients only.These results suggest that the pathogenesis of GS is different from that of OLP.GS is a unique inflammatory disorder characterized by dysfunction of Th2 and Th17 immune reactions via abnormal T-B cell interaction.

View Article: PubMed Central - PubMed

Affiliation: From the Section of Oral and Maxillofacial Oncology (TM, MM, SK, J-NH, SF, MO, AT, MY, SN); Section of Oral and Maxillofacial Surgery (YO); and Division of Maxillofacial Diagnostic and Surgical Sciences, Laboratory of Oral Pathology, Faculty of Dental Science, Kyushu University, Fukuoka, Japan (YK).

ABSTRACT
We described and analyzed the pathogenic difference between Good syndrome (GS) and oral lichen planus (OLP) in oral mucosa. Good syndrome (GS) is a rare disease characterized by B and T cell immunodeficiency associated with hypogammaglobulinemia and thymoma. GS patients frequently develop oral lichenoid lesions with lymphocytic infiltration beneath the basal layer. Oral lichen planus (OLP) is a chronic inflammatory disease of the oral mucosa characterized by destruction of basal cells by Langerhans cells, macrophages, and T lymphocytes. Although the histological features of the lesions of both diseases are very similar, the pathogenesis of GS in the oral mucosa remains unknown. In this study, we thus investigated the expression of infiltrating lymphocyte subsets (CD3, CD20, CD4, and CD8) and T helper (Th) cytokines including interferon (IFN)-γ (Th1 type), interleukin (IL)-4 (Th2 type), IL-17 (Th17 type), and IL-10 (regulatory T cell type) by immunohistochemistry in buccal mucosa specimens from 2 GS patients compared with 15 OLP patients. All patients showed a predominance of CD3 T cells over CD20 B cells, and CD4 Th cells over CD8 cytotoxic T cells. This polarization was especially prominent in GS. IFN-γ and IL-10 were strongly detected in the infiltrating lymphocytes of all patients. However, IL-4 and IL-17 were detected in OLP patients only. These results suggest that the pathogenesis of GS is different from that of OLP. GS is a unique inflammatory disorder characterized by dysfunction of Th2 and Th17 immune reactions via abnormal T-B cell interaction.

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Lymphocyte subsets in the buccal mucosa from patients with GS and OLP. (A) Buccal mucosa specimens from representative patients with GS and OLP were stained with HE (a and j) and anti-CD3 (b, c, k, and l), anti-CD20 (d, e, m, and n), anti-CD4 (f, g, o, and p), and anti-CD8 (h, i, q, and r) monoclonal antibodies. Counterstaining with Mayer hematoxylin was subsequently performed. Scale bars, 100 μm. (B) Number of CD3, CD20, CD4, and CD8+ cells per HPF were counted in 1-mm2 sections from 5 different areas from patients with GS (n = 2) and OLP (n = 15). GS = Good syndrome, HPF = high-power field, OLP = oral lichen planus.
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Figure 3: Lymphocyte subsets in the buccal mucosa from patients with GS and OLP. (A) Buccal mucosa specimens from representative patients with GS and OLP were stained with HE (a and j) and anti-CD3 (b, c, k, and l), anti-CD20 (d, e, m, and n), anti-CD4 (f, g, o, and p), and anti-CD8 (h, i, q, and r) monoclonal antibodies. Counterstaining with Mayer hematoxylin was subsequently performed. Scale bars, 100 μm. (B) Number of CD3, CD20, CD4, and CD8+ cells per HPF were counted in 1-mm2 sections from 5 different areas from patients with GS (n = 2) and OLP (n = 15). GS = Good syndrome, HPF = high-power field, OLP = oral lichen planus.

Mentions: Representative histological findings in the buccal mucosa are shown in Fig. 3. Both GS and OLP patients showed typical band-like lymphocytic infiltration and diffuse infiltration into the connective tissue papillae and the lamina propria. The GS patient showed diffuse infiltration of CD3+ T cells in the lamina propria, whereas CD20+ B cells were rarely seen. In contrast, The OLP patients showed strong, band-like infiltrations of CD3+ T cells and diffuse infiltrations of CD20+ B cells in the sub-basal region. A slight predominance of CD4+ T cells over CD8+ T cells was observed in the OLP patients, whereas an increased predominance of CD4+ cells over CD8+ cells was seen in the GS patient (Fig. 3B).


Cytokine profiles contribute to understanding the pathogenic difference between Good syndrome and oral lichen planus: two case reports and literature review.

Maehara T, Moriyama M, Kawano S, Hayashida JN, Furukawa S, Ohta M, Tanaka A, Yamauchi M, Ohyama Y, Kiyoshima T, Nakamura S - Medicine (Baltimore) (2015)

Lymphocyte subsets in the buccal mucosa from patients with GS and OLP. (A) Buccal mucosa specimens from representative patients with GS and OLP were stained with HE (a and j) and anti-CD3 (b, c, k, and l), anti-CD20 (d, e, m, and n), anti-CD4 (f, g, o, and p), and anti-CD8 (h, i, q, and r) monoclonal antibodies. Counterstaining with Mayer hematoxylin was subsequently performed. Scale bars, 100 μm. (B) Number of CD3, CD20, CD4, and CD8+ cells per HPF were counted in 1-mm2 sections from 5 different areas from patients with GS (n = 2) and OLP (n = 15). GS = Good syndrome, HPF = high-power field, OLP = oral lichen planus.
© Copyright Policy - open-access
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4554038&req=5

Figure 3: Lymphocyte subsets in the buccal mucosa from patients with GS and OLP. (A) Buccal mucosa specimens from representative patients with GS and OLP were stained with HE (a and j) and anti-CD3 (b, c, k, and l), anti-CD20 (d, e, m, and n), anti-CD4 (f, g, o, and p), and anti-CD8 (h, i, q, and r) monoclonal antibodies. Counterstaining with Mayer hematoxylin was subsequently performed. Scale bars, 100 μm. (B) Number of CD3, CD20, CD4, and CD8+ cells per HPF were counted in 1-mm2 sections from 5 different areas from patients with GS (n = 2) and OLP (n = 15). GS = Good syndrome, HPF = high-power field, OLP = oral lichen planus.
Mentions: Representative histological findings in the buccal mucosa are shown in Fig. 3. Both GS and OLP patients showed typical band-like lymphocytic infiltration and diffuse infiltration into the connective tissue papillae and the lamina propria. The GS patient showed diffuse infiltration of CD3+ T cells in the lamina propria, whereas CD20+ B cells were rarely seen. In contrast, The OLP patients showed strong, band-like infiltrations of CD3+ T cells and diffuse infiltrations of CD20+ B cells in the sub-basal region. A slight predominance of CD4+ T cells over CD8+ T cells was observed in the OLP patients, whereas an increased predominance of CD4+ cells over CD8+ cells was seen in the GS patient (Fig. 3B).

Bottom Line: However, IL-4 and IL-17 were detected in OLP patients only.These results suggest that the pathogenesis of GS is different from that of OLP.GS is a unique inflammatory disorder characterized by dysfunction of Th2 and Th17 immune reactions via abnormal T-B cell interaction.

View Article: PubMed Central - PubMed

Affiliation: From the Section of Oral and Maxillofacial Oncology (TM, MM, SK, J-NH, SF, MO, AT, MY, SN); Section of Oral and Maxillofacial Surgery (YO); and Division of Maxillofacial Diagnostic and Surgical Sciences, Laboratory of Oral Pathology, Faculty of Dental Science, Kyushu University, Fukuoka, Japan (YK).

ABSTRACT
We described and analyzed the pathogenic difference between Good syndrome (GS) and oral lichen planus (OLP) in oral mucosa. Good syndrome (GS) is a rare disease characterized by B and T cell immunodeficiency associated with hypogammaglobulinemia and thymoma. GS patients frequently develop oral lichenoid lesions with lymphocytic infiltration beneath the basal layer. Oral lichen planus (OLP) is a chronic inflammatory disease of the oral mucosa characterized by destruction of basal cells by Langerhans cells, macrophages, and T lymphocytes. Although the histological features of the lesions of both diseases are very similar, the pathogenesis of GS in the oral mucosa remains unknown. In this study, we thus investigated the expression of infiltrating lymphocyte subsets (CD3, CD20, CD4, and CD8) and T helper (Th) cytokines including interferon (IFN)-γ (Th1 type), interleukin (IL)-4 (Th2 type), IL-17 (Th17 type), and IL-10 (regulatory T cell type) by immunohistochemistry in buccal mucosa specimens from 2 GS patients compared with 15 OLP patients. All patients showed a predominance of CD3 T cells over CD20 B cells, and CD4 Th cells over CD8 cytotoxic T cells. This polarization was especially prominent in GS. IFN-γ and IL-10 were strongly detected in the infiltrating lymphocytes of all patients. However, IL-4 and IL-17 were detected in OLP patients only. These results suggest that the pathogenesis of GS is different from that of OLP. GS is a unique inflammatory disorder characterized by dysfunction of Th2 and Th17 immune reactions via abnormal T-B cell interaction.

Show MeSH
Related in: MedlinePlus