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Association between catalase gene polymorphisms and risk of chronic hepatitis B, hepatitis B virus-related liver cirrhosis and hepatocellular carcinoma in Guangxi population: a case-control study.

Liu Y, Xie L, Zhao J, Huang X, Song L, Luo J, Ma L, Li S, Qin X - Medicine (Baltimore) (2015)

Bottom Line: Furthermore, we found 1 high-risk haplotype GTA for CHB (OR = 1.45, 95% CI = 1.05-2.01) and 1 protective haplotype GCA for HCC risk (OR = 0.67, 95% CI = 0.52-0.87).We did not found any significant difference in CAT rs1001179 and rs7943316 polymorphisms between controls and cases.Our findings suggest that the CAT rs769217 T allele is associated with increased risk of CHB, HBV-LC, and HBV-HCC in Guangxi population.

View Article: PubMed Central - PubMed

Affiliation: From the Department of Clinical Laboratory, First Affiliated Hospital of Guangxi Medical University, Nanning, Guangxi, China.

ABSTRACT
Reactive oxygen species (ROS) play critical roles in hepatocarcinogenesis. The catalase (CAT) enzyme is involved in the repair of ROS. Therefore, we investigate the association between CAT gene polymorphisms and the risk of hepatocellular carcinoma (HCC). A total of 715 subjects were divided into 4 groups: 111 chronic hepatitis B (CHB) patients, 90 hepatitis B virus (HBV)-related liver cirrhosis (LC) patients, 266 HBV-HCC patients, and 248 healthy controls. The polymerase chain reaction-restriction fragment length polymorphism strategy was used to detect CAT gene rs1001179, rs769217, and rs7943316 polymorphisms. Binary logistic regression analyses adjusting for sex, age, ethnicity, smoking and alcohol consumption, and body mass index suggested that subjects carrying the rs769217 T allele were at marginally increased risk of CHB, LC, and HCC, with adjusted odds ratios (ORs) of 1.51 (95% confidence interval [CI] = 1.04-2.20, P = 0.029), 1.48 (95% CI = 1.03-2.14, P = 0.035), and 1.51 (95% CI = 1.14-1.98, P = 0.004), respectively. Similarly, those individuals carrying the rs769217 TT genotype had a moderately increased risk of CHB, LC, and HCC, with adjusted ORs of 2.11 (95% CI = 1.05-4.22, P = 0.035), 2.00 (95% CI, 1.01-3.95, P = 0.047), and 1.93 (95% CI = 1.14-3.28, P = 0.015), respectively. Moreover, subjects carrying the rs769217 CT genotype and at least 1 copy of the T allele (dominant model) were 1.78 times and 1.83 times more likely to develop HCC, respectively (OR = 1.78, 95% CI = 1.16-2.73, P = 0.009 and OR = 1.83, 95% CI = 1.23-2.71, P = 0.003). This association between CAT rs769217 T alleles and HCC risk is significantly strengthened among men, nonsmokers, nondrinkers, and among individuals <50 years of age. Furthermore, we found 1 high-risk haplotype GTA for CHB (OR = 1.45, 95% CI = 1.05-2.01) and 1 protective haplotype GCA for HCC risk (OR = 0.67, 95% CI = 0.52-0.87). We did not found any significant difference in CAT rs1001179 and rs7943316 polymorphisms between controls and cases. Our findings suggest that the CAT rs769217 T allele is associated with increased risk of CHB, HBV-LC, and HBV-HCC in Guangxi population.

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PCR-RFLP assay for analyzing the rs1001179, rs769217, and rs7943316 polymorphisms in CAT gene. (A) rs1001179—lanes M: DNA marker; lanes 1, 3, 4, 5, 7, and 8 show GG genotype; lanes 2 and 6 show AG genotype; lane 9 shows AA genotype; lane 10 shows negative control. (B) rs769217—lanes M: DNA marker; lanes 1, 4, 5, 7, 8, and 9 show CT genotype; lanes 2 and 6 show CC genotype; lane 3 shows TT genotypes; lanes 10 shows negative control. (C) rs7943316—lanes M: DNA marker; lanes 1 and 8 show AA genotype; lanes 2, 3, 7, and 9 show AT genotype; lane 4, 5, and 6 shows TT genotypes; lanes 10 shows negative control. PCR-RFLP = polymerase chain reaction-restriction fragment length polymorphism.
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Figure 1: PCR-RFLP assay for analyzing the rs1001179, rs769217, and rs7943316 polymorphisms in CAT gene. (A) rs1001179—lanes M: DNA marker; lanes 1, 3, 4, 5, 7, and 8 show GG genotype; lanes 2 and 6 show AG genotype; lane 9 shows AA genotype; lane 10 shows negative control. (B) rs769217—lanes M: DNA marker; lanes 1, 4, 5, 7, 8, and 9 show CT genotype; lanes 2 and 6 show CC genotype; lane 3 shows TT genotypes; lanes 10 shows negative control. (C) rs7943316—lanes M: DNA marker; lanes 1 and 8 show AA genotype; lanes 2, 3, 7, and 9 show AT genotype; lane 4, 5, and 6 shows TT genotypes; lanes 10 shows negative control. PCR-RFLP = polymerase chain reaction-restriction fragment length polymorphism.

Mentions: Digested fragments were separated by electrophoresis in 2% agarose gel containing GoldView I (Beijing Solarbio Science & Technology Co., Ltd., Beijing, China) and the fragments were visualized by the UV transilluminator (Figure 1). To control the quality of genotyping, a negative control was performed in each genotyping assay. The negative control utilized a PCR-amplified DNA product without the restriction enzymes.


Association between catalase gene polymorphisms and risk of chronic hepatitis B, hepatitis B virus-related liver cirrhosis and hepatocellular carcinoma in Guangxi population: a case-control study.

Liu Y, Xie L, Zhao J, Huang X, Song L, Luo J, Ma L, Li S, Qin X - Medicine (Baltimore) (2015)

PCR-RFLP assay for analyzing the rs1001179, rs769217, and rs7943316 polymorphisms in CAT gene. (A) rs1001179—lanes M: DNA marker; lanes 1, 3, 4, 5, 7, and 8 show GG genotype; lanes 2 and 6 show AG genotype; lane 9 shows AA genotype; lane 10 shows negative control. (B) rs769217—lanes M: DNA marker; lanes 1, 4, 5, 7, 8, and 9 show CT genotype; lanes 2 and 6 show CC genotype; lane 3 shows TT genotypes; lanes 10 shows negative control. (C) rs7943316—lanes M: DNA marker; lanes 1 and 8 show AA genotype; lanes 2, 3, 7, and 9 show AT genotype; lane 4, 5, and 6 shows TT genotypes; lanes 10 shows negative control. PCR-RFLP = polymerase chain reaction-restriction fragment length polymorphism.
© Copyright Policy - open-access
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4554034&req=5

Figure 1: PCR-RFLP assay for analyzing the rs1001179, rs769217, and rs7943316 polymorphisms in CAT gene. (A) rs1001179—lanes M: DNA marker; lanes 1, 3, 4, 5, 7, and 8 show GG genotype; lanes 2 and 6 show AG genotype; lane 9 shows AA genotype; lane 10 shows negative control. (B) rs769217—lanes M: DNA marker; lanes 1, 4, 5, 7, 8, and 9 show CT genotype; lanes 2 and 6 show CC genotype; lane 3 shows TT genotypes; lanes 10 shows negative control. (C) rs7943316—lanes M: DNA marker; lanes 1 and 8 show AA genotype; lanes 2, 3, 7, and 9 show AT genotype; lane 4, 5, and 6 shows TT genotypes; lanes 10 shows negative control. PCR-RFLP = polymerase chain reaction-restriction fragment length polymorphism.
Mentions: Digested fragments were separated by electrophoresis in 2% agarose gel containing GoldView I (Beijing Solarbio Science & Technology Co., Ltd., Beijing, China) and the fragments were visualized by the UV transilluminator (Figure 1). To control the quality of genotyping, a negative control was performed in each genotyping assay. The negative control utilized a PCR-amplified DNA product without the restriction enzymes.

Bottom Line: Furthermore, we found 1 high-risk haplotype GTA for CHB (OR = 1.45, 95% CI = 1.05-2.01) and 1 protective haplotype GCA for HCC risk (OR = 0.67, 95% CI = 0.52-0.87).We did not found any significant difference in CAT rs1001179 and rs7943316 polymorphisms between controls and cases.Our findings suggest that the CAT rs769217 T allele is associated with increased risk of CHB, HBV-LC, and HBV-HCC in Guangxi population.

View Article: PubMed Central - PubMed

Affiliation: From the Department of Clinical Laboratory, First Affiliated Hospital of Guangxi Medical University, Nanning, Guangxi, China.

ABSTRACT
Reactive oxygen species (ROS) play critical roles in hepatocarcinogenesis. The catalase (CAT) enzyme is involved in the repair of ROS. Therefore, we investigate the association between CAT gene polymorphisms and the risk of hepatocellular carcinoma (HCC). A total of 715 subjects were divided into 4 groups: 111 chronic hepatitis B (CHB) patients, 90 hepatitis B virus (HBV)-related liver cirrhosis (LC) patients, 266 HBV-HCC patients, and 248 healthy controls. The polymerase chain reaction-restriction fragment length polymorphism strategy was used to detect CAT gene rs1001179, rs769217, and rs7943316 polymorphisms. Binary logistic regression analyses adjusting for sex, age, ethnicity, smoking and alcohol consumption, and body mass index suggested that subjects carrying the rs769217 T allele were at marginally increased risk of CHB, LC, and HCC, with adjusted odds ratios (ORs) of 1.51 (95% confidence interval [CI] = 1.04-2.20, P = 0.029), 1.48 (95% CI = 1.03-2.14, P = 0.035), and 1.51 (95% CI = 1.14-1.98, P = 0.004), respectively. Similarly, those individuals carrying the rs769217 TT genotype had a moderately increased risk of CHB, LC, and HCC, with adjusted ORs of 2.11 (95% CI = 1.05-4.22, P = 0.035), 2.00 (95% CI, 1.01-3.95, P = 0.047), and 1.93 (95% CI = 1.14-3.28, P = 0.015), respectively. Moreover, subjects carrying the rs769217 CT genotype and at least 1 copy of the T allele (dominant model) were 1.78 times and 1.83 times more likely to develop HCC, respectively (OR = 1.78, 95% CI = 1.16-2.73, P = 0.009 and OR = 1.83, 95% CI = 1.23-2.71, P = 0.003). This association between CAT rs769217 T alleles and HCC risk is significantly strengthened among men, nonsmokers, nondrinkers, and among individuals <50 years of age. Furthermore, we found 1 high-risk haplotype GTA for CHB (OR = 1.45, 95% CI = 1.05-2.01) and 1 protective haplotype GCA for HCC risk (OR = 0.67, 95% CI = 0.52-0.87). We did not found any significant difference in CAT rs1001179 and rs7943316 polymorphisms between controls and cases. Our findings suggest that the CAT rs769217 T allele is associated with increased risk of CHB, HBV-LC, and HBV-HCC in Guangxi population.

Show MeSH
Related in: MedlinePlus