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Differential expression of hypertension-associated microRNAs in the plasma of patients with white coat hypertension.

Cengiz M, Karatas OF, Koparir E, Yavuzer S, Ali C, Yavuzer H, Kirat E, Karter Y, Ozen M - Medicine (Baltimore) (2015)

Bottom Line: MiR-21, miR-122, miR-637, and let-7e expression levels were significantly upregulated in the HT group compared with the NT groups (P = 0.017, P = 0.022, P = 0.048, and P = 0.013, respectively).MiR-122 and miR-637 expressions were also significantly upregulated in the WCH group compared with the NT group (P = 0.048 and P = 0.039, respectively).MiR-296-5p expression level was significantly downregulated in HT patients and upregulated in the WCH patients compared with the NT group (P = 0.049 and P = 0.039, respectively).

View Article: PubMed Central - PubMed

Affiliation: From the Department of Internal Medicine (MC, SY, CA, HY, YK), Cerrahpasa Medical School, Istanbul University; Department of Medical Genetics (OFK, E Koparir, E Kirat, MO), Istanbul University Cerrahpasa Medical School, Istanbul; Molecular Biology and Genetics Department (OFK), Erzurum Technical University, Erzurum, Turkey; Department of Pathology & Immunology (MO), Baylor College of Medicine, Houston, Texas; and Department of Molecular Biology and Genetics (MO), Biruni University, Istanbul, Turkey.

ABSTRACT
White coat hypertension (WCH) is a high cardiovascular risk condition, and a fundamental understanding of the cause and pathophysiology of the disorder is still lacking. Recent studies demonstrated that microRNAs (miRNAs) are involved in hypertension; however, the roles of miRNAs in WCH are not known. The expressions of selected 10 miRNAs were investigated independently in plasma samples from 30 hypertension (HT) patients, 30 WCH patients, and 30 normotensive (NT) subjects. MiR-21, miR-122, miR-637, and let-7e expression levels were significantly upregulated in the HT group compared with the NT groups (P = 0.017, P = 0.022, P = 0.048, and P = 0.013, respectively). MiR-122 and miR-637 expressions were also significantly upregulated in the WCH group compared with the NT group (P = 0.048 and P = 0.039, respectively). MiR-296-5p expression level was significantly downregulated in HT patients and upregulated in the WCH patients compared with the NT group (P = 0.049 and P = 0.039, respectively). Additionally, the ambulatory 24-hour and daytime systolic and diastolic blood pressures were negatively correlated with miR-296-5p. MiR-296 and miR-637 had area under the curve (AUC) values of 0.778 and 0.774, respectively, which demonstrates their sufficiency to distinguish WCH from NT individuals. MiR-296 and miR-637 had AUC values of 0.868 and 0.680, respectively, which shows their potential to distinguish WCH from HT individuals. We report for the first time a plasma miRNA profile for WCH patients and demonstrate a novel link between miRNA and WCH. These findings may reveal crucial insights into the development of WCH.

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ROC analysis of miR-296-5p and miR-637, curves for individual miRNAs and their cooperative power to discriminate 2 sets of patients comprised NT and WCH (A). ROC analysis of miR-122, curves for individual miRNAs, and their cooperative power to discriminate 2 sets of patients comprised NT and HT (B). ROC analysis of miR-296-5p and miR-637, curves for individual miRNAs, and their cooperative power to discriminate 2 sets of patients comprised WCH and HT (C). HT = hypertension, NT = normotensive, ROC = receiver operating characteristic, WCH = white coat hypertension.
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Figure 3: ROC analysis of miR-296-5p and miR-637, curves for individual miRNAs and their cooperative power to discriminate 2 sets of patients comprised NT and WCH (A). ROC analysis of miR-122, curves for individual miRNAs, and their cooperative power to discriminate 2 sets of patients comprised NT and HT (B). ROC analysis of miR-296-5p and miR-637, curves for individual miRNAs, and their cooperative power to discriminate 2 sets of patients comprised WCH and HT (C). HT = hypertension, NT = normotensive, ROC = receiver operating characteristic, WCH = white coat hypertension.

Mentions: To test the power of the miRNAs for distinguishing NT, WCH, and HT groups, ROC analysis was performed. MiR-296 and miR-637 had AUC values of 0.778 and 0.774, respectively, which demonstrates their sufficiency to distinguish WCH from NT individuals (Figure 3A). MiR-122 had AUC values of 0.688, which demonstrates its potential to distinguish HT from NT individuals on its own (Figure 3B). MiR-296 and miR-637, with AUC values of 0.868 and 0.680, respectively, also had enough power to distinguish WCH from HT individuals on their own (Table 4 and Figure 3C).


Differential expression of hypertension-associated microRNAs in the plasma of patients with white coat hypertension.

Cengiz M, Karatas OF, Koparir E, Yavuzer S, Ali C, Yavuzer H, Kirat E, Karter Y, Ozen M - Medicine (Baltimore) (2015)

ROC analysis of miR-296-5p and miR-637, curves for individual miRNAs and their cooperative power to discriminate 2 sets of patients comprised NT and WCH (A). ROC analysis of miR-122, curves for individual miRNAs, and their cooperative power to discriminate 2 sets of patients comprised NT and HT (B). ROC analysis of miR-296-5p and miR-637, curves for individual miRNAs, and their cooperative power to discriminate 2 sets of patients comprised WCH and HT (C). HT = hypertension, NT = normotensive, ROC = receiver operating characteristic, WCH = white coat hypertension.
© Copyright Policy - open-access
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4554020&req=5

Figure 3: ROC analysis of miR-296-5p and miR-637, curves for individual miRNAs and their cooperative power to discriminate 2 sets of patients comprised NT and WCH (A). ROC analysis of miR-122, curves for individual miRNAs, and their cooperative power to discriminate 2 sets of patients comprised NT and HT (B). ROC analysis of miR-296-5p and miR-637, curves for individual miRNAs, and their cooperative power to discriminate 2 sets of patients comprised WCH and HT (C). HT = hypertension, NT = normotensive, ROC = receiver operating characteristic, WCH = white coat hypertension.
Mentions: To test the power of the miRNAs for distinguishing NT, WCH, and HT groups, ROC analysis was performed. MiR-296 and miR-637 had AUC values of 0.778 and 0.774, respectively, which demonstrates their sufficiency to distinguish WCH from NT individuals (Figure 3A). MiR-122 had AUC values of 0.688, which demonstrates its potential to distinguish HT from NT individuals on its own (Figure 3B). MiR-296 and miR-637, with AUC values of 0.868 and 0.680, respectively, also had enough power to distinguish WCH from HT individuals on their own (Table 4 and Figure 3C).

Bottom Line: MiR-21, miR-122, miR-637, and let-7e expression levels were significantly upregulated in the HT group compared with the NT groups (P = 0.017, P = 0.022, P = 0.048, and P = 0.013, respectively).MiR-122 and miR-637 expressions were also significantly upregulated in the WCH group compared with the NT group (P = 0.048 and P = 0.039, respectively).MiR-296-5p expression level was significantly downregulated in HT patients and upregulated in the WCH patients compared with the NT group (P = 0.049 and P = 0.039, respectively).

View Article: PubMed Central - PubMed

Affiliation: From the Department of Internal Medicine (MC, SY, CA, HY, YK), Cerrahpasa Medical School, Istanbul University; Department of Medical Genetics (OFK, E Koparir, E Kirat, MO), Istanbul University Cerrahpasa Medical School, Istanbul; Molecular Biology and Genetics Department (OFK), Erzurum Technical University, Erzurum, Turkey; Department of Pathology & Immunology (MO), Baylor College of Medicine, Houston, Texas; and Department of Molecular Biology and Genetics (MO), Biruni University, Istanbul, Turkey.

ABSTRACT
White coat hypertension (WCH) is a high cardiovascular risk condition, and a fundamental understanding of the cause and pathophysiology of the disorder is still lacking. Recent studies demonstrated that microRNAs (miRNAs) are involved in hypertension; however, the roles of miRNAs in WCH are not known. The expressions of selected 10 miRNAs were investigated independently in plasma samples from 30 hypertension (HT) patients, 30 WCH patients, and 30 normotensive (NT) subjects. MiR-21, miR-122, miR-637, and let-7e expression levels were significantly upregulated in the HT group compared with the NT groups (P = 0.017, P = 0.022, P = 0.048, and P = 0.013, respectively). MiR-122 and miR-637 expressions were also significantly upregulated in the WCH group compared with the NT group (P = 0.048 and P = 0.039, respectively). MiR-296-5p expression level was significantly downregulated in HT patients and upregulated in the WCH patients compared with the NT group (P = 0.049 and P = 0.039, respectively). Additionally, the ambulatory 24-hour and daytime systolic and diastolic blood pressures were negatively correlated with miR-296-5p. MiR-296 and miR-637 had area under the curve (AUC) values of 0.778 and 0.774, respectively, which demonstrates their sufficiency to distinguish WCH from NT individuals. MiR-296 and miR-637 had AUC values of 0.868 and 0.680, respectively, which shows their potential to distinguish WCH from HT individuals. We report for the first time a plasma miRNA profile for WCH patients and demonstrate a novel link between miRNA and WCH. These findings may reveal crucial insights into the development of WCH.

Show MeSH
Related in: MedlinePlus