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Differential expression of hypertension-associated microRNAs in the plasma of patients with white coat hypertension.

Cengiz M, Karatas OF, Koparir E, Yavuzer S, Ali C, Yavuzer H, Kirat E, Karter Y, Ozen M - Medicine (Baltimore) (2015)

Bottom Line: MiR-21, miR-122, miR-637, and let-7e expression levels were significantly upregulated in the HT group compared with the NT groups (P = 0.017, P = 0.022, P = 0.048, and P = 0.013, respectively).MiR-122 and miR-637 expressions were also significantly upregulated in the WCH group compared with the NT group (P = 0.048 and P = 0.039, respectively).MiR-296-5p expression level was significantly downregulated in HT patients and upregulated in the WCH patients compared with the NT group (P = 0.049 and P = 0.039, respectively).

View Article: PubMed Central - PubMed

Affiliation: From the Department of Internal Medicine (MC, SY, CA, HY, YK), Cerrahpasa Medical School, Istanbul University; Department of Medical Genetics (OFK, E Koparir, E Kirat, MO), Istanbul University Cerrahpasa Medical School, Istanbul; Molecular Biology and Genetics Department (OFK), Erzurum Technical University, Erzurum, Turkey; Department of Pathology & Immunology (MO), Baylor College of Medicine, Houston, Texas; and Department of Molecular Biology and Genetics (MO), Biruni University, Istanbul, Turkey.

ABSTRACT
White coat hypertension (WCH) is a high cardiovascular risk condition, and a fundamental understanding of the cause and pathophysiology of the disorder is still lacking. Recent studies demonstrated that microRNAs (miRNAs) are involved in hypertension; however, the roles of miRNAs in WCH are not known. The expressions of selected 10 miRNAs were investigated independently in plasma samples from 30 hypertension (HT) patients, 30 WCH patients, and 30 normotensive (NT) subjects. MiR-21, miR-122, miR-637, and let-7e expression levels were significantly upregulated in the HT group compared with the NT groups (P = 0.017, P = 0.022, P = 0.048, and P = 0.013, respectively). MiR-122 and miR-637 expressions were also significantly upregulated in the WCH group compared with the NT group (P = 0.048 and P = 0.039, respectively). MiR-296-5p expression level was significantly downregulated in HT patients and upregulated in the WCH patients compared with the NT group (P = 0.049 and P = 0.039, respectively). Additionally, the ambulatory 24-hour and daytime systolic and diastolic blood pressures were negatively correlated with miR-296-5p. MiR-296 and miR-637 had area under the curve (AUC) values of 0.778 and 0.774, respectively, which demonstrates their sufficiency to distinguish WCH from NT individuals. MiR-296 and miR-637 had AUC values of 0.868 and 0.680, respectively, which shows their potential to distinguish WCH from HT individuals. We report for the first time a plasma miRNA profile for WCH patients and demonstrate a novel link between miRNA and WCH. These findings may reveal crucial insights into the development of WCH.

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Related in: MedlinePlus

Relative expression levels of miRNAs, ∗P < 0.05. HT = hypertension, NT = normotensive, WCH = white coat hypertension.
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Figure 1: Relative expression levels of miRNAs, ∗P < 0.05. HT = hypertension, NT = normotensive, WCH = white coat hypertension.

Mentions: The relative expression levels of selected miRNAs in study groups are shown in Table 2. let-7e (P = 0.013), miR-21 (P = 0.017), miR-122 (P = 0.022), and miR-637 (P = 0.048) displayed increased relative expression levels in HT patients compared with those of NT group. Expression levels of miR-21 and let-7e did not show any alteration in WCH group in comparison with NT individuals. MiR-122 (P = 0.048) and miR-637 (P = 0.039) showed increased expression in the WCH group. On the contrary, miR-296-5p expression was significantly reduced in HT patients in comparison with NT individuals (P = 0.049). Interestingly, its expression is strongly upregulated in WCH group (P = 0.039). No significant difference was observed between the control and other groups with regard to miR-125a, miR-126, miR-130a, miR-155, and miR-195 expression levels (Figure 1).


Differential expression of hypertension-associated microRNAs in the plasma of patients with white coat hypertension.

Cengiz M, Karatas OF, Koparir E, Yavuzer S, Ali C, Yavuzer H, Kirat E, Karter Y, Ozen M - Medicine (Baltimore) (2015)

Relative expression levels of miRNAs, ∗P < 0.05. HT = hypertension, NT = normotensive, WCH = white coat hypertension.
© Copyright Policy - open-access
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4554020&req=5

Figure 1: Relative expression levels of miRNAs, ∗P < 0.05. HT = hypertension, NT = normotensive, WCH = white coat hypertension.
Mentions: The relative expression levels of selected miRNAs in study groups are shown in Table 2. let-7e (P = 0.013), miR-21 (P = 0.017), miR-122 (P = 0.022), and miR-637 (P = 0.048) displayed increased relative expression levels in HT patients compared with those of NT group. Expression levels of miR-21 and let-7e did not show any alteration in WCH group in comparison with NT individuals. MiR-122 (P = 0.048) and miR-637 (P = 0.039) showed increased expression in the WCH group. On the contrary, miR-296-5p expression was significantly reduced in HT patients in comparison with NT individuals (P = 0.049). Interestingly, its expression is strongly upregulated in WCH group (P = 0.039). No significant difference was observed between the control and other groups with regard to miR-125a, miR-126, miR-130a, miR-155, and miR-195 expression levels (Figure 1).

Bottom Line: MiR-21, miR-122, miR-637, and let-7e expression levels were significantly upregulated in the HT group compared with the NT groups (P = 0.017, P = 0.022, P = 0.048, and P = 0.013, respectively).MiR-122 and miR-637 expressions were also significantly upregulated in the WCH group compared with the NT group (P = 0.048 and P = 0.039, respectively).MiR-296-5p expression level was significantly downregulated in HT patients and upregulated in the WCH patients compared with the NT group (P = 0.049 and P = 0.039, respectively).

View Article: PubMed Central - PubMed

Affiliation: From the Department of Internal Medicine (MC, SY, CA, HY, YK), Cerrahpasa Medical School, Istanbul University; Department of Medical Genetics (OFK, E Koparir, E Kirat, MO), Istanbul University Cerrahpasa Medical School, Istanbul; Molecular Biology and Genetics Department (OFK), Erzurum Technical University, Erzurum, Turkey; Department of Pathology & Immunology (MO), Baylor College of Medicine, Houston, Texas; and Department of Molecular Biology and Genetics (MO), Biruni University, Istanbul, Turkey.

ABSTRACT
White coat hypertension (WCH) is a high cardiovascular risk condition, and a fundamental understanding of the cause and pathophysiology of the disorder is still lacking. Recent studies demonstrated that microRNAs (miRNAs) are involved in hypertension; however, the roles of miRNAs in WCH are not known. The expressions of selected 10 miRNAs were investigated independently in plasma samples from 30 hypertension (HT) patients, 30 WCH patients, and 30 normotensive (NT) subjects. MiR-21, miR-122, miR-637, and let-7e expression levels were significantly upregulated in the HT group compared with the NT groups (P = 0.017, P = 0.022, P = 0.048, and P = 0.013, respectively). MiR-122 and miR-637 expressions were also significantly upregulated in the WCH group compared with the NT group (P = 0.048 and P = 0.039, respectively). MiR-296-5p expression level was significantly downregulated in HT patients and upregulated in the WCH patients compared with the NT group (P = 0.049 and P = 0.039, respectively). Additionally, the ambulatory 24-hour and daytime systolic and diastolic blood pressures were negatively correlated with miR-296-5p. MiR-296 and miR-637 had area under the curve (AUC) values of 0.778 and 0.774, respectively, which demonstrates their sufficiency to distinguish WCH from NT individuals. MiR-296 and miR-637 had AUC values of 0.868 and 0.680, respectively, which shows their potential to distinguish WCH from HT individuals. We report for the first time a plasma miRNA profile for WCH patients and demonstrate a novel link between miRNA and WCH. These findings may reveal crucial insights into the development of WCH.

Show MeSH
Related in: MedlinePlus