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Effect of Momordica charantia fruit extract on vascular complication in type 1 diabetic rats.

Abas R, Othman F, Thent ZC - EXCLI J (2015)

Bottom Line: DM-MC group showed a significant decrease (P < 0.05) in blood pressure, total cholesterol and triglyceride levels compared to the DM-Ctrl group.Aortic tissue NO level was significantly increased and malondialdehyde level was decreased in the DM-MC group.In summary, treatment with the MC fruit extract exerted the significant vasculoprotective effect in the type 1 diabetic rat model.

View Article: PubMed Central - PubMed

Affiliation: Department of Anatomy, Universiti Kebangsaan Malaysia, Jalan Yaacob Latif, 56000 Cheras, Malaysia ; Department of Human Anatomy, Faculty of Medicine and Health Science, Universiti Putra Malaysia, 43400 Serdang, Selangor.

ABSTRACT
Diabetes mellitus is one of the risk factors in the development of vascular complications. Decreased nitric oxide (NO) production and increased lipid peroxidation in diabetes mellitus are the dominant exaggerating factors. Mormodica charantia (MC) was proven to be useful in improving diabetes mellitus and its complications. In the present study, a total of 40 male Sprague-Dawley rats were used. Diabetes was induced by a single dose (50 mg/kg) of streptozotocin (STZ), intramuscularly. Following 4 weeks of STZ induction, the animals were equally divided into five groups (n = 8); Control group (Ctrl), control group treated with MC (Ctrl-MC), diabetic untreated group (DM-Ctrl), diabetic group treated with MC (DM-MC) and diabetic group treated with metformin 150 g/kg (DM-Met). Oral administration of the MC fruit extract (1.5 g/kg) was continued for 28 days. DM-MC group showed a significant decrease (P < 0.05) in blood pressure, total cholesterol and triglyceride levels compared to the DM-Ctrl group. Aortic tissue NO level was significantly increased and malondialdehyde level was decreased in the DM-MC group. Immunohistochemical staining showed an increase in eNOS expression in the endothelial lining of the DM-MC group. Similarly, morphological deterioration of the aortic tissues was reverted to normal. In summary, treatment with the MC fruit extract exerted the significant vasculoprotective effect in the type 1 diabetic rat model.

No MeSH data available.


Related in: MedlinePlus

Photomicrograph showing transverse sections of the thoracic aorta under Orcinol staining. a(Ctrl), b(Ctrl-MC), c(DM-Ctrl), d(DM-MC), e(DM-Met). Note the reduced tortuosity of elastic fibres in c(DM-Ctrl). TM= Tunica Media, EC= Endothelial cell, L= Lumen. LM X 200
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Figure 6: Photomicrograph showing transverse sections of the thoracic aorta under Orcinol staining. a(Ctrl), b(Ctrl-MC), c(DM-Ctrl), d(DM-MC), e(DM-Met). Note the reduced tortuosity of elastic fibres in c(DM-Ctrl). TM= Tunica Media, EC= Endothelial cell, L= Lumen. LM X 200

Mentions: Loss of endothelial cells in TI, disrupted elastic lamellae and increased thickness were observed in the DM-Ctrl group. However, DM-MC group showed less pathological changes compared to the DM-Ctrl group. The presence of endothelial cells in TI and reduced TM thickness was observed in the DM-MC group under H&E staining. Similar features were observed in the DM-Met group (Figure 4(Fig. 4)). Alcian blue staining is a special staining used to stain the connective tissue deposition, especially acid mucopolysaccharides. The increased acid mucopolysaccharides deposition was observed in the DM-Ctrl group which appeared blue in colour. However, less acid mucopolysaccharides deposition with blue staining in the DM-MC group was observed compared to the DM-Ctrl group (Figure 5(Fig. 5)). Orcinol staining is used to delineate the deposition of elastic fibres in the large arteries. The histological findings of thoracic aorta in the DM-Ctrl group showed decrease in tortuosity of the elastic fibres. However, these degenerative changes were found to be improved in the DM-MC group. Tortuous elastic fibres with intact endothelial cells was observed in the DM-MC group. Similar findings were observed in the DM-Met group (Figure 6(Fig. 6)).


Effect of Momordica charantia fruit extract on vascular complication in type 1 diabetic rats.

Abas R, Othman F, Thent ZC - EXCLI J (2015)

Photomicrograph showing transverse sections of the thoracic aorta under Orcinol staining. a(Ctrl), b(Ctrl-MC), c(DM-Ctrl), d(DM-MC), e(DM-Met). Note the reduced tortuosity of elastic fibres in c(DM-Ctrl). TM= Tunica Media, EC= Endothelial cell, L= Lumen. LM X 200
© Copyright Policy - open-access
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4553902&req=5

Figure 6: Photomicrograph showing transverse sections of the thoracic aorta under Orcinol staining. a(Ctrl), b(Ctrl-MC), c(DM-Ctrl), d(DM-MC), e(DM-Met). Note the reduced tortuosity of elastic fibres in c(DM-Ctrl). TM= Tunica Media, EC= Endothelial cell, L= Lumen. LM X 200
Mentions: Loss of endothelial cells in TI, disrupted elastic lamellae and increased thickness were observed in the DM-Ctrl group. However, DM-MC group showed less pathological changes compared to the DM-Ctrl group. The presence of endothelial cells in TI and reduced TM thickness was observed in the DM-MC group under H&E staining. Similar features were observed in the DM-Met group (Figure 4(Fig. 4)). Alcian blue staining is a special staining used to stain the connective tissue deposition, especially acid mucopolysaccharides. The increased acid mucopolysaccharides deposition was observed in the DM-Ctrl group which appeared blue in colour. However, less acid mucopolysaccharides deposition with blue staining in the DM-MC group was observed compared to the DM-Ctrl group (Figure 5(Fig. 5)). Orcinol staining is used to delineate the deposition of elastic fibres in the large arteries. The histological findings of thoracic aorta in the DM-Ctrl group showed decrease in tortuosity of the elastic fibres. However, these degenerative changes were found to be improved in the DM-MC group. Tortuous elastic fibres with intact endothelial cells was observed in the DM-MC group. Similar findings were observed in the DM-Met group (Figure 6(Fig. 6)).

Bottom Line: DM-MC group showed a significant decrease (P < 0.05) in blood pressure, total cholesterol and triglyceride levels compared to the DM-Ctrl group.Aortic tissue NO level was significantly increased and malondialdehyde level was decreased in the DM-MC group.In summary, treatment with the MC fruit extract exerted the significant vasculoprotective effect in the type 1 diabetic rat model.

View Article: PubMed Central - PubMed

Affiliation: Department of Anatomy, Universiti Kebangsaan Malaysia, Jalan Yaacob Latif, 56000 Cheras, Malaysia ; Department of Human Anatomy, Faculty of Medicine and Health Science, Universiti Putra Malaysia, 43400 Serdang, Selangor.

ABSTRACT
Diabetes mellitus is one of the risk factors in the development of vascular complications. Decreased nitric oxide (NO) production and increased lipid peroxidation in diabetes mellitus are the dominant exaggerating factors. Mormodica charantia (MC) was proven to be useful in improving diabetes mellitus and its complications. In the present study, a total of 40 male Sprague-Dawley rats were used. Diabetes was induced by a single dose (50 mg/kg) of streptozotocin (STZ), intramuscularly. Following 4 weeks of STZ induction, the animals were equally divided into five groups (n = 8); Control group (Ctrl), control group treated with MC (Ctrl-MC), diabetic untreated group (DM-Ctrl), diabetic group treated with MC (DM-MC) and diabetic group treated with metformin 150 g/kg (DM-Met). Oral administration of the MC fruit extract (1.5 g/kg) was continued for 28 days. DM-MC group showed a significant decrease (P < 0.05) in blood pressure, total cholesterol and triglyceride levels compared to the DM-Ctrl group. Aortic tissue NO level was significantly increased and malondialdehyde level was decreased in the DM-MC group. Immunohistochemical staining showed an increase in eNOS expression in the endothelial lining of the DM-MC group. Similarly, morphological deterioration of the aortic tissues was reverted to normal. In summary, treatment with the MC fruit extract exerted the significant vasculoprotective effect in the type 1 diabetic rat model.

No MeSH data available.


Related in: MedlinePlus