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Effect of Momordica charantia fruit extract on vascular complication in type 1 diabetic rats.

Abas R, Othman F, Thent ZC - EXCLI J (2015)

Bottom Line: DM-MC group showed a significant decrease (P < 0.05) in blood pressure, total cholesterol and triglyceride levels compared to the DM-Ctrl group.Aortic tissue NO level was significantly increased and malondialdehyde level was decreased in the DM-MC group.In summary, treatment with the MC fruit extract exerted the significant vasculoprotective effect in the type 1 diabetic rat model.

View Article: PubMed Central - PubMed

Affiliation: Department of Anatomy, Universiti Kebangsaan Malaysia, Jalan Yaacob Latif, 56000 Cheras, Malaysia ; Department of Human Anatomy, Faculty of Medicine and Health Science, Universiti Putra Malaysia, 43400 Serdang, Selangor.

ABSTRACT
Diabetes mellitus is one of the risk factors in the development of vascular complications. Decreased nitric oxide (NO) production and increased lipid peroxidation in diabetes mellitus are the dominant exaggerating factors. Mormodica charantia (MC) was proven to be useful in improving diabetes mellitus and its complications. In the present study, a total of 40 male Sprague-Dawley rats were used. Diabetes was induced by a single dose (50 mg/kg) of streptozotocin (STZ), intramuscularly. Following 4 weeks of STZ induction, the animals were equally divided into five groups (n = 8); Control group (Ctrl), control group treated with MC (Ctrl-MC), diabetic untreated group (DM-Ctrl), diabetic group treated with MC (DM-MC) and diabetic group treated with metformin 150 g/kg (DM-Met). Oral administration of the MC fruit extract (1.5 g/kg) was continued for 28 days. DM-MC group showed a significant decrease (P < 0.05) in blood pressure, total cholesterol and triglyceride levels compared to the DM-Ctrl group. Aortic tissue NO level was significantly increased and malondialdehyde level was decreased in the DM-MC group. Immunohistochemical staining showed an increase in eNOS expression in the endothelial lining of the DM-MC group. Similarly, morphological deterioration of the aortic tissues was reverted to normal. In summary, treatment with the MC fruit extract exerted the significant vasculoprotective effect in the type 1 diabetic rat model.

No MeSH data available.


Related in: MedlinePlus

Photomicrograph showing cross sections of the thoracic aorta under immunohistochemical staining for eNOS expression. a(Ctrl), b(Ctrl-MC), c(DM-Ctrl), d(DM-MC), e(DM-Met). Note the increase eNOS expression in endothelial lining (stained black, blue arrows) in c(DM-Ctrl). LM X 200
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Figure 3: Photomicrograph showing cross sections of the thoracic aorta under immunohistochemical staining for eNOS expression. a(Ctrl), b(Ctrl-MC), c(DM-Ctrl), d(DM-MC), e(DM-Met). Note the increase eNOS expression in endothelial lining (stained black, blue arrows) in c(DM-Ctrl). LM X 200

Mentions: Under immunohistochemical staining, endothelial nitric oxide synthase (eNOS) expression was shown as black in colour in the endothelial lining of the tunica intima. Findings showed little or no eNOS expression in the DM-Ctrl group. The thickness of TM also prominently increased. There was reduced elasticity of the elastic fibres in the DM-Ctrl group. However, the aortic tissue in the DM-MC group showed an increase in the eNOS expression, less TM thickness and more elasticity compared to the DM-Ctrl group. Furthermore, DM-Met group showed increased expression of eNOS in the endothelial lining. No changes in eNOS expression were observed in the non-diabetic groups (Figure 3(Fig. 3)).


Effect of Momordica charantia fruit extract on vascular complication in type 1 diabetic rats.

Abas R, Othman F, Thent ZC - EXCLI J (2015)

Photomicrograph showing cross sections of the thoracic aorta under immunohistochemical staining for eNOS expression. a(Ctrl), b(Ctrl-MC), c(DM-Ctrl), d(DM-MC), e(DM-Met). Note the increase eNOS expression in endothelial lining (stained black, blue arrows) in c(DM-Ctrl). LM X 200
© Copyright Policy - open-access
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4553902&req=5

Figure 3: Photomicrograph showing cross sections of the thoracic aorta under immunohistochemical staining for eNOS expression. a(Ctrl), b(Ctrl-MC), c(DM-Ctrl), d(DM-MC), e(DM-Met). Note the increase eNOS expression in endothelial lining (stained black, blue arrows) in c(DM-Ctrl). LM X 200
Mentions: Under immunohistochemical staining, endothelial nitric oxide synthase (eNOS) expression was shown as black in colour in the endothelial lining of the tunica intima. Findings showed little or no eNOS expression in the DM-Ctrl group. The thickness of TM also prominently increased. There was reduced elasticity of the elastic fibres in the DM-Ctrl group. However, the aortic tissue in the DM-MC group showed an increase in the eNOS expression, less TM thickness and more elasticity compared to the DM-Ctrl group. Furthermore, DM-Met group showed increased expression of eNOS in the endothelial lining. No changes in eNOS expression were observed in the non-diabetic groups (Figure 3(Fig. 3)).

Bottom Line: DM-MC group showed a significant decrease (P < 0.05) in blood pressure, total cholesterol and triglyceride levels compared to the DM-Ctrl group.Aortic tissue NO level was significantly increased and malondialdehyde level was decreased in the DM-MC group.In summary, treatment with the MC fruit extract exerted the significant vasculoprotective effect in the type 1 diabetic rat model.

View Article: PubMed Central - PubMed

Affiliation: Department of Anatomy, Universiti Kebangsaan Malaysia, Jalan Yaacob Latif, 56000 Cheras, Malaysia ; Department of Human Anatomy, Faculty of Medicine and Health Science, Universiti Putra Malaysia, 43400 Serdang, Selangor.

ABSTRACT
Diabetes mellitus is one of the risk factors in the development of vascular complications. Decreased nitric oxide (NO) production and increased lipid peroxidation in diabetes mellitus are the dominant exaggerating factors. Mormodica charantia (MC) was proven to be useful in improving diabetes mellitus and its complications. In the present study, a total of 40 male Sprague-Dawley rats were used. Diabetes was induced by a single dose (50 mg/kg) of streptozotocin (STZ), intramuscularly. Following 4 weeks of STZ induction, the animals were equally divided into five groups (n = 8); Control group (Ctrl), control group treated with MC (Ctrl-MC), diabetic untreated group (DM-Ctrl), diabetic group treated with MC (DM-MC) and diabetic group treated with metformin 150 g/kg (DM-Met). Oral administration of the MC fruit extract (1.5 g/kg) was continued for 28 days. DM-MC group showed a significant decrease (P < 0.05) in blood pressure, total cholesterol and triglyceride levels compared to the DM-Ctrl group. Aortic tissue NO level was significantly increased and malondialdehyde level was decreased in the DM-MC group. Immunohistochemical staining showed an increase in eNOS expression in the endothelial lining of the DM-MC group. Similarly, morphological deterioration of the aortic tissues was reverted to normal. In summary, treatment with the MC fruit extract exerted the significant vasculoprotective effect in the type 1 diabetic rat model.

No MeSH data available.


Related in: MedlinePlus