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Evaluation of offspring sex ratio, sex hormones and antioxidant enzymes following exposure to methyl tertiary butyl ether in adult male Sprague-Dawley rats.

Khalili L, Gholami S, Ansari-Lari M - EXCLI J (2015)

Bottom Line: There was significant decreasing trend for luteinizing hormone (LH) and testosterone in experimental groups (rs = -0.50, P = 0.030 and rs = -0.67, P = 0.002, respectively).However, decrease in glutathione peroxidase (GPX) was observed in all treatment groups compared with control which was significant in 400 mg/kg/day MTBE group (P = 0.016).The results of the present study, need to be clarified in the future studies.

View Article: PubMed Central - PubMed

Affiliation: Department of Basic Sciences, School of Veterinary Medicine, Shiraz University, Shiraz, PO Box 1731, Postal code 71345, Iran.

ABSTRACT
Methyl tertiary butyl ether (MTBE) is an oxygenated fuel additive which has been used widely in many parts of the world. This experiment was performed to determine the effect of MTBE on offspring sex ratio, sex hormones and antioxidant enzymes. A total of 20 adult Sprague-Dawley male rats were divided into four groups and received 0, 400, 800 and 1600 mg/kg/day MTBE by gavages for 30 consecutive days. At the end of the experiment, blood samples were taken for determination of sex hormones and antioxidant enzymes. Then, male rats were mated with healthy unexposed female rats and sex of offspring was determined after birth. Sex ratio was 0.48, 0.50, 0.43 and 0.50 in 0, 400, 800 and 1600 mg/kg/day MTBE groups, respectively (P = 0.91). There was significant decreasing trend for luteinizing hormone (LH) and testosterone in experimental groups (rs = -0.50, P = 0.030 and rs = -0.67, P = 0.002, respectively). No changes were observed for superoxide dismutase. However, decrease in glutathione peroxidase (GPX) was observed in all treatment groups compared with control which was significant in 400 mg/kg/day MTBE group (P = 0.016). The present study showed that paternal exposure to oral MTBE has no effect on offspring sex ratio; while, MTBE exposure could exert dose-dependent changes in serum testosterone and LH in treatment groups. The results of the present study, need to be clarified in the future studies.

No MeSH data available.


Related in: MedlinePlus

Comparison of serum luteinizing hormone (LH, mIU/ml), follicle stimulating hormone (FSH, mIU/ml), testosterone (ng/ml), superoxide dismutase (SOD, U/gHb) and glutathione peroxidase (GPX, U/gHb) in MTBE-treated (mg/kg/day) adult Sprague-Dawley male rats
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T2: Comparison of serum luteinizing hormone (LH, mIU/ml), follicle stimulating hormone (FSH, mIU/ml), testosterone (ng/ml), superoxide dismutase (SOD, U/gHb) and glutathione peroxidase (GPX, U/gHb) in MTBE-treated (mg/kg/day) adult Sprague-Dawley male rats

Mentions: Comparisons of sex hormones between study groups are presented in Table 2(Tab. 2). There was no significant difference (P = 0.33) between study groups for level of follicle-stimulating hormone (FSH). Luteinizing hormone (LH) was significantly lower in 800 mg/kg/day (P = 0.032), and testosterone was lower in 1600 mg/kg/day MTBE group compared with control one (P = 0.032). Spearman’s rho correlation coefficient for levels of serum FSH, LH and testosterone with ordered dose groups were -0.43 (P = 0.064), -0.50 (P = 0.030) and -0.67 (P = 0.002), respectively. These decreasing trends with increasing dose (negative dose-response relationship) are clearly depicted in Figure 1(Fig. 1) which shows the quartiles of data in each experimental group.


Evaluation of offspring sex ratio, sex hormones and antioxidant enzymes following exposure to methyl tertiary butyl ether in adult male Sprague-Dawley rats.

Khalili L, Gholami S, Ansari-Lari M - EXCLI J (2015)

Comparison of serum luteinizing hormone (LH, mIU/ml), follicle stimulating hormone (FSH, mIU/ml), testosterone (ng/ml), superoxide dismutase (SOD, U/gHb) and glutathione peroxidase (GPX, U/gHb) in MTBE-treated (mg/kg/day) adult Sprague-Dawley male rats
© Copyright Policy - open-access
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4553898&req=5

T2: Comparison of serum luteinizing hormone (LH, mIU/ml), follicle stimulating hormone (FSH, mIU/ml), testosterone (ng/ml), superoxide dismutase (SOD, U/gHb) and glutathione peroxidase (GPX, U/gHb) in MTBE-treated (mg/kg/day) adult Sprague-Dawley male rats
Mentions: Comparisons of sex hormones between study groups are presented in Table 2(Tab. 2). There was no significant difference (P = 0.33) between study groups for level of follicle-stimulating hormone (FSH). Luteinizing hormone (LH) was significantly lower in 800 mg/kg/day (P = 0.032), and testosterone was lower in 1600 mg/kg/day MTBE group compared with control one (P = 0.032). Spearman’s rho correlation coefficient for levels of serum FSH, LH and testosterone with ordered dose groups were -0.43 (P = 0.064), -0.50 (P = 0.030) and -0.67 (P = 0.002), respectively. These decreasing trends with increasing dose (negative dose-response relationship) are clearly depicted in Figure 1(Fig. 1) which shows the quartiles of data in each experimental group.

Bottom Line: There was significant decreasing trend for luteinizing hormone (LH) and testosterone in experimental groups (rs = -0.50, P = 0.030 and rs = -0.67, P = 0.002, respectively).However, decrease in glutathione peroxidase (GPX) was observed in all treatment groups compared with control which was significant in 400 mg/kg/day MTBE group (P = 0.016).The results of the present study, need to be clarified in the future studies.

View Article: PubMed Central - PubMed

Affiliation: Department of Basic Sciences, School of Veterinary Medicine, Shiraz University, Shiraz, PO Box 1731, Postal code 71345, Iran.

ABSTRACT
Methyl tertiary butyl ether (MTBE) is an oxygenated fuel additive which has been used widely in many parts of the world. This experiment was performed to determine the effect of MTBE on offspring sex ratio, sex hormones and antioxidant enzymes. A total of 20 adult Sprague-Dawley male rats were divided into four groups and received 0, 400, 800 and 1600 mg/kg/day MTBE by gavages for 30 consecutive days. At the end of the experiment, blood samples were taken for determination of sex hormones and antioxidant enzymes. Then, male rats were mated with healthy unexposed female rats and sex of offspring was determined after birth. Sex ratio was 0.48, 0.50, 0.43 and 0.50 in 0, 400, 800 and 1600 mg/kg/day MTBE groups, respectively (P = 0.91). There was significant decreasing trend for luteinizing hormone (LH) and testosterone in experimental groups (rs = -0.50, P = 0.030 and rs = -0.67, P = 0.002, respectively). No changes were observed for superoxide dismutase. However, decrease in glutathione peroxidase (GPX) was observed in all treatment groups compared with control which was significant in 400 mg/kg/day MTBE group (P = 0.016). The present study showed that paternal exposure to oral MTBE has no effect on offspring sex ratio; while, MTBE exposure could exert dose-dependent changes in serum testosterone and LH in treatment groups. The results of the present study, need to be clarified in the future studies.

No MeSH data available.


Related in: MedlinePlus