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Evaluation of offspring sex ratio, sex hormones and antioxidant enzymes following exposure to methyl tertiary butyl ether in adult male Sprague-Dawley rats.

Khalili L, Gholami S, Ansari-Lari M - EXCLI J (2015)

Bottom Line: There was significant decreasing trend for luteinizing hormone (LH) and testosterone in experimental groups (rs = -0.50, P = 0.030 and rs = -0.67, P = 0.002, respectively).However, decrease in glutathione peroxidase (GPX) was observed in all treatment groups compared with control which was significant in 400 mg/kg/day MTBE group (P = 0.016).The results of the present study, need to be clarified in the future studies.

View Article: PubMed Central - PubMed

Affiliation: Department of Basic Sciences, School of Veterinary Medicine, Shiraz University, Shiraz, PO Box 1731, Postal code 71345, Iran.

ABSTRACT
Methyl tertiary butyl ether (MTBE) is an oxygenated fuel additive which has been used widely in many parts of the world. This experiment was performed to determine the effect of MTBE on offspring sex ratio, sex hormones and antioxidant enzymes. A total of 20 adult Sprague-Dawley male rats were divided into four groups and received 0, 400, 800 and 1600 mg/kg/day MTBE by gavages for 30 consecutive days. At the end of the experiment, blood samples were taken for determination of sex hormones and antioxidant enzymes. Then, male rats were mated with healthy unexposed female rats and sex of offspring was determined after birth. Sex ratio was 0.48, 0.50, 0.43 and 0.50 in 0, 400, 800 and 1600 mg/kg/day MTBE groups, respectively (P = 0.91). There was significant decreasing trend for luteinizing hormone (LH) and testosterone in experimental groups (rs = -0.50, P = 0.030 and rs = -0.67, P = 0.002, respectively). No changes were observed for superoxide dismutase. However, decrease in glutathione peroxidase (GPX) was observed in all treatment groups compared with control which was significant in 400 mg/kg/day MTBE group (P = 0.016). The present study showed that paternal exposure to oral MTBE has no effect on offspring sex ratio; while, MTBE exposure could exert dose-dependent changes in serum testosterone and LH in treatment groups. The results of the present study, need to be clarified in the future studies.

No MeSH data available.


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Summary statistics for reproductive outcomes in MTBE-treated adult Sprague-Dawley male rats
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T1: Summary statistics for reproductive outcomes in MTBE-treated adult Sprague-Dawley male rats

Mentions: Overall, 149 male and 155 female were produced by experimental groups. No stillbirth or gross anomaly was detected in experimental groups. Summary statistics for reproductive parameters are displayed in Table 1(Tab. 1). No significant difference between groups sex ratio (P = 0.91) or number of pups at birth (P = 0.11) was detected (Table 1(Tab. 1)). Fertility index based on pregnant females was equal to 81.8 % for the first three experimental groups and 54.5 % for 1600 mg/kg MTBE group (P = 0.22). When fertility index was calculated based on fertile males, it was 100 % for the first three groups and 60 % for 1600 mg/kg MTBE group (P = 0.058).


Evaluation of offspring sex ratio, sex hormones and antioxidant enzymes following exposure to methyl tertiary butyl ether in adult male Sprague-Dawley rats.

Khalili L, Gholami S, Ansari-Lari M - EXCLI J (2015)

Summary statistics for reproductive outcomes in MTBE-treated adult Sprague-Dawley male rats
© Copyright Policy - open-access
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4553898&req=5

T1: Summary statistics for reproductive outcomes in MTBE-treated adult Sprague-Dawley male rats
Mentions: Overall, 149 male and 155 female were produced by experimental groups. No stillbirth or gross anomaly was detected in experimental groups. Summary statistics for reproductive parameters are displayed in Table 1(Tab. 1). No significant difference between groups sex ratio (P = 0.91) or number of pups at birth (P = 0.11) was detected (Table 1(Tab. 1)). Fertility index based on pregnant females was equal to 81.8 % for the first three experimental groups and 54.5 % for 1600 mg/kg MTBE group (P = 0.22). When fertility index was calculated based on fertile males, it was 100 % for the first three groups and 60 % for 1600 mg/kg MTBE group (P = 0.058).

Bottom Line: There was significant decreasing trend for luteinizing hormone (LH) and testosterone in experimental groups (rs = -0.50, P = 0.030 and rs = -0.67, P = 0.002, respectively).However, decrease in glutathione peroxidase (GPX) was observed in all treatment groups compared with control which was significant in 400 mg/kg/day MTBE group (P = 0.016).The results of the present study, need to be clarified in the future studies.

View Article: PubMed Central - PubMed

Affiliation: Department of Basic Sciences, School of Veterinary Medicine, Shiraz University, Shiraz, PO Box 1731, Postal code 71345, Iran.

ABSTRACT
Methyl tertiary butyl ether (MTBE) is an oxygenated fuel additive which has been used widely in many parts of the world. This experiment was performed to determine the effect of MTBE on offspring sex ratio, sex hormones and antioxidant enzymes. A total of 20 adult Sprague-Dawley male rats were divided into four groups and received 0, 400, 800 and 1600 mg/kg/day MTBE by gavages for 30 consecutive days. At the end of the experiment, blood samples were taken for determination of sex hormones and antioxidant enzymes. Then, male rats were mated with healthy unexposed female rats and sex of offspring was determined after birth. Sex ratio was 0.48, 0.50, 0.43 and 0.50 in 0, 400, 800 and 1600 mg/kg/day MTBE groups, respectively (P = 0.91). There was significant decreasing trend for luteinizing hormone (LH) and testosterone in experimental groups (rs = -0.50, P = 0.030 and rs = -0.67, P = 0.002, respectively). No changes were observed for superoxide dismutase. However, decrease in glutathione peroxidase (GPX) was observed in all treatment groups compared with control which was significant in 400 mg/kg/day MTBE group (P = 0.016). The present study showed that paternal exposure to oral MTBE has no effect on offspring sex ratio; while, MTBE exposure could exert dose-dependent changes in serum testosterone and LH in treatment groups. The results of the present study, need to be clarified in the future studies.

No MeSH data available.


Related in: MedlinePlus