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Biodegradation and detoxification of textile azo dyes by bacterial consortium under sequential microaerophilic/aerobic processes.

Lade H, Kadam A, Paul D, Govindwar S - EXCLI J (2015)

Bottom Line: RB 5 (0.08 mM), RO 16 (0.06 mM), DR 78 (0.07 mM) and DR 81 (0.09 mM) resulted into the formation of aromatic amines.Additionally, 62-72 % reduction in total organic carbon content was observed in all the dyes decolorized broths under sequential microaerophilic/aerobic processes suggesting the efficacy of method in mineralization of dyes.Notable induction within the levels of azoreductase and NADH-DCIP reductase (97 and 229 % for RB 5, 55 and 160 % for RO 16, 63 and 196 % for DR 78, 108 and 258 % for DR 81) observed under sequential microaerophilic/aerobic processes suggested their critical involvements in the initial breakdown of azo bonds, whereas, a slight increase in the levels of laccase and veratryl alcohol oxidase confirmed subsequent oxidation of formed amines.

View Article: PubMed Central - PubMed

Affiliation: Department of Environmental Engineering, Konkuk University, Seoul-143-701, Korea.

ABSTRACT
Release of textile azo dyes to the environment is an issue of health concern while the use of microorganisms has proved to be the best option for remediation. Thus, in the present study, a bacterial consortium consisting of Providencia rettgeri strain HSL1 and Pseudomonas sp. SUK1 has been investigated for degradation and detoxification of structurally different azo dyes. The consortium showed 98-99 % decolorization of all the selected azo dyes viz. Reactive Black 5 (RB 5), Reactive Orange 16 (RO 16), Disperse Red 78 (DR 78) and Direct Red 81 (DR 81) within 12 to 30 h at 100 mg L(-1) concentration at 30 ± 0.2 °C under microaerophilic, sequential aerobic/microaerophilic and microaerophilic/aerobic processes. However, decolorization under microaerophilic conditions viz. RB 5 (0.26 mM), RO 16 (0.18 mM), DR 78 (0.20 mM) and DR 81 (0.23 mM) and sequential aerobic/microaerophilic processes viz. RB 5 (0.08 mM), RO 16 (0.06 mM), DR 78 (0.07 mM) and DR 81 (0.09 mM) resulted into the formation of aromatic amines. In distinction, sequential microaerophilic/ aerobic process doesn't show the formation of amines. Additionally, 62-72 % reduction in total organic carbon content was observed in all the dyes decolorized broths under sequential microaerophilic/aerobic processes suggesting the efficacy of method in mineralization of dyes. Notable induction within the levels of azoreductase and NADH-DCIP reductase (97 and 229 % for RB 5, 55 and 160 % for RO 16, 63 and 196 % for DR 78, 108 and 258 % for DR 81) observed under sequential microaerophilic/aerobic processes suggested their critical involvements in the initial breakdown of azo bonds, whereas, a slight increase in the levels of laccase and veratryl alcohol oxidase confirmed subsequent oxidation of formed amines. Also, the acute toxicity assay with Daphnia magna revealed the nontoxic nature of the dye-degraded metabolites under sequential microaerophilic/aerobic processes. As biodegradation under sequential microaerophilic/aerobic process completely detoxified all the selected textile azo dyes, further efforts should be made to implement such methods for large scale dye wastewater treatment technologies.

No MeSH data available.


Related in: MedlinePlus

The structural information of textile azo dyes used in this study
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T1: The structural information of textile azo dyes used in this study

Mentions: The textile azo dyes viz. C.I. Reactive black 5 (RB 5), C.I. Reactive orange 16 (RO 16), C.I. Disperse red 78 (DR 78) and C.I. Direct red 81 (DR 81) were kindly provided by Mahesh Textile Processors, Ichalkaranji, India. The structural information of dyes is shown in Table 1(Tab. 1). Veratryl alcohol, 2,2’-Azino-bis (3-ethylbenzothiazoline-6-sulfonic acid) (ABTS), methyl red, nicotinamide adenine dinucleotide (NADH), dichlorophenol indophenols (DCIP) and dehydrated microbiological medium nutrient broth (NB) were obtained from HiMedia Laboratories Pvt. Ltd., Mumbai, India. Chloranil, Dimethylformamide and Aniline-2-sulfonic were purchased from Sigma-Aldrich (St. Louis, MO, USA).


Biodegradation and detoxification of textile azo dyes by bacterial consortium under sequential microaerophilic/aerobic processes.

Lade H, Kadam A, Paul D, Govindwar S - EXCLI J (2015)

The structural information of textile azo dyes used in this study
© Copyright Policy - open-access
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4553892&req=5

T1: The structural information of textile azo dyes used in this study
Mentions: The textile azo dyes viz. C.I. Reactive black 5 (RB 5), C.I. Reactive orange 16 (RO 16), C.I. Disperse red 78 (DR 78) and C.I. Direct red 81 (DR 81) were kindly provided by Mahesh Textile Processors, Ichalkaranji, India. The structural information of dyes is shown in Table 1(Tab. 1). Veratryl alcohol, 2,2’-Azino-bis (3-ethylbenzothiazoline-6-sulfonic acid) (ABTS), methyl red, nicotinamide adenine dinucleotide (NADH), dichlorophenol indophenols (DCIP) and dehydrated microbiological medium nutrient broth (NB) were obtained from HiMedia Laboratories Pvt. Ltd., Mumbai, India. Chloranil, Dimethylformamide and Aniline-2-sulfonic were purchased from Sigma-Aldrich (St. Louis, MO, USA).

Bottom Line: RB 5 (0.08 mM), RO 16 (0.06 mM), DR 78 (0.07 mM) and DR 81 (0.09 mM) resulted into the formation of aromatic amines.Additionally, 62-72 % reduction in total organic carbon content was observed in all the dyes decolorized broths under sequential microaerophilic/aerobic processes suggesting the efficacy of method in mineralization of dyes.Notable induction within the levels of azoreductase and NADH-DCIP reductase (97 and 229 % for RB 5, 55 and 160 % for RO 16, 63 and 196 % for DR 78, 108 and 258 % for DR 81) observed under sequential microaerophilic/aerobic processes suggested their critical involvements in the initial breakdown of azo bonds, whereas, a slight increase in the levels of laccase and veratryl alcohol oxidase confirmed subsequent oxidation of formed amines.

View Article: PubMed Central - PubMed

Affiliation: Department of Environmental Engineering, Konkuk University, Seoul-143-701, Korea.

ABSTRACT
Release of textile azo dyes to the environment is an issue of health concern while the use of microorganisms has proved to be the best option for remediation. Thus, in the present study, a bacterial consortium consisting of Providencia rettgeri strain HSL1 and Pseudomonas sp. SUK1 has been investigated for degradation and detoxification of structurally different azo dyes. The consortium showed 98-99 % decolorization of all the selected azo dyes viz. Reactive Black 5 (RB 5), Reactive Orange 16 (RO 16), Disperse Red 78 (DR 78) and Direct Red 81 (DR 81) within 12 to 30 h at 100 mg L(-1) concentration at 30 ± 0.2 °C under microaerophilic, sequential aerobic/microaerophilic and microaerophilic/aerobic processes. However, decolorization under microaerophilic conditions viz. RB 5 (0.26 mM), RO 16 (0.18 mM), DR 78 (0.20 mM) and DR 81 (0.23 mM) and sequential aerobic/microaerophilic processes viz. RB 5 (0.08 mM), RO 16 (0.06 mM), DR 78 (0.07 mM) and DR 81 (0.09 mM) resulted into the formation of aromatic amines. In distinction, sequential microaerophilic/ aerobic process doesn't show the formation of amines. Additionally, 62-72 % reduction in total organic carbon content was observed in all the dyes decolorized broths under sequential microaerophilic/aerobic processes suggesting the efficacy of method in mineralization of dyes. Notable induction within the levels of azoreductase and NADH-DCIP reductase (97 and 229 % for RB 5, 55 and 160 % for RO 16, 63 and 196 % for DR 78, 108 and 258 % for DR 81) observed under sequential microaerophilic/aerobic processes suggested their critical involvements in the initial breakdown of azo bonds, whereas, a slight increase in the levels of laccase and veratryl alcohol oxidase confirmed subsequent oxidation of formed amines. Also, the acute toxicity assay with Daphnia magna revealed the nontoxic nature of the dye-degraded metabolites under sequential microaerophilic/aerobic processes. As biodegradation under sequential microaerophilic/aerobic process completely detoxified all the selected textile azo dyes, further efforts should be made to implement such methods for large scale dye wastewater treatment technologies.

No MeSH data available.


Related in: MedlinePlus