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Host Searching and Aggregation Activity of Recently Fed and Unfed Bed Bugs (Cimex lectularius L.).

Reis MD, Miller DM - Insects (2011)

Bottom Line: Groups of starved, virgin adult male or female bed bugs were stimulated to search for a host by the presence of a heated artificial feeder.Groups of male and female bed bugs that were unable to obtain a blood meal continued to search in the arena for the majority of the scotophase.Overall, the proportion of bed bugs aggregating in shelters during the scotophase was significantly greater for those that had fed successfully than those that had not.

View Article: PubMed Central - PubMed

Affiliation: Department of Entomology, Virginia Tech, 216A Price Hall, Blacksburg, VA 24061, USA. matthewreis174@gmail.com.

ABSTRACT
Groups of starved, virgin adult male or female bed bugs were stimulated to search for a host by the presence of a heated artificial feeder. Some of the bed bug groups were allowed to obtain a blood meal and some were not. After the removal of the feeder, bed bugs were observed throughout the scotophase to record their searching and aggregation behavior. Groups of male and female bed bugs that were unable to obtain a blood meal continued to search in the arena for the majority of the scotophase. Bed bugs that were able to obtain a blood meal returned to their shelter to aggregate 30 min after feeding. Overall, the proportion of bed bugs aggregating in shelters during the scotophase was significantly greater for those that had fed successfully than those that had not. However, all bed bugs, regardless of feeding status, began to return to shelters to aggregate 2 h prior to the photophase.

No MeSH data available.


Related in: MedlinePlus

Bioassay arenas used to document bed bug scotophase activity. Bed bugs were located inside the filter paper shelter at the right: (a) An arena where bed bugs were able to climb up a paper tab to reach an artificial feeder; (b) An arena where paper tab was absent so bed bugs could detect the heat of the feeder but not reach it. Illustration: WR Kuhn.
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f1-insects-02-00186: Bioassay arenas used to document bed bug scotophase activity. Bed bugs were located inside the filter paper shelter at the right: (a) An arena where bed bugs were able to climb up a paper tab to reach an artificial feeder; (b) An arena where paper tab was absent so bed bugs could detect the heat of the feeder but not reach it. Illustration: WR Kuhn.

Mentions: Bed bugs are known to be stimulated by host cues, including heat [6]. To stimulate the bed bug searching behavior, an artificial feeder containing heated chicken blood was placed directly on the cloth mesh in the center of the arena lid at 21:00. The bed bugs would then leave their shelter to search for the heat source. Half of the arenas (five replicates) were provided with a filter paper tab hanging from the bottom of the cloth mesh. The bed bugs were able to climb up the tab and access a blood meal from the feeder (Figure 1a). These bed bugs were given 30 min to feed to repletion and drop off of the tab. The second half of the arenas (five replicates) were not provided with a paper tab so the bed bugs would be stimulated, but not able to obtain a blood meal from the artificial feeder (Figure 1b). The bed bugs without the paper tab were allowed to forage (unsuccessfully) for 30 min. After this time, the artificial feeder was removed and a digital video camera (Sony DCR-TRV250 Digital Video Recorder, Sony) was placed over each arena to record all bed bug behavior. The bed bugs' behavior was captured from 21:00 to 11:00.


Host Searching and Aggregation Activity of Recently Fed and Unfed Bed Bugs (Cimex lectularius L.).

Reis MD, Miller DM - Insects (2011)

Bioassay arenas used to document bed bug scotophase activity. Bed bugs were located inside the filter paper shelter at the right: (a) An arena where bed bugs were able to climb up a paper tab to reach an artificial feeder; (b) An arena where paper tab was absent so bed bugs could detect the heat of the feeder but not reach it. Illustration: WR Kuhn.
© Copyright Policy
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4553457&req=5

f1-insects-02-00186: Bioassay arenas used to document bed bug scotophase activity. Bed bugs were located inside the filter paper shelter at the right: (a) An arena where bed bugs were able to climb up a paper tab to reach an artificial feeder; (b) An arena where paper tab was absent so bed bugs could detect the heat of the feeder but not reach it. Illustration: WR Kuhn.
Mentions: Bed bugs are known to be stimulated by host cues, including heat [6]. To stimulate the bed bug searching behavior, an artificial feeder containing heated chicken blood was placed directly on the cloth mesh in the center of the arena lid at 21:00. The bed bugs would then leave their shelter to search for the heat source. Half of the arenas (five replicates) were provided with a filter paper tab hanging from the bottom of the cloth mesh. The bed bugs were able to climb up the tab and access a blood meal from the feeder (Figure 1a). These bed bugs were given 30 min to feed to repletion and drop off of the tab. The second half of the arenas (five replicates) were not provided with a paper tab so the bed bugs would be stimulated, but not able to obtain a blood meal from the artificial feeder (Figure 1b). The bed bugs without the paper tab were allowed to forage (unsuccessfully) for 30 min. After this time, the artificial feeder was removed and a digital video camera (Sony DCR-TRV250 Digital Video Recorder, Sony) was placed over each arena to record all bed bug behavior. The bed bugs' behavior was captured from 21:00 to 11:00.

Bottom Line: Groups of starved, virgin adult male or female bed bugs were stimulated to search for a host by the presence of a heated artificial feeder.Groups of male and female bed bugs that were unable to obtain a blood meal continued to search in the arena for the majority of the scotophase.Overall, the proportion of bed bugs aggregating in shelters during the scotophase was significantly greater for those that had fed successfully than those that had not.

View Article: PubMed Central - PubMed

Affiliation: Department of Entomology, Virginia Tech, 216A Price Hall, Blacksburg, VA 24061, USA. matthewreis174@gmail.com.

ABSTRACT
Groups of starved, virgin adult male or female bed bugs were stimulated to search for a host by the presence of a heated artificial feeder. Some of the bed bug groups were allowed to obtain a blood meal and some were not. After the removal of the feeder, bed bugs were observed throughout the scotophase to record their searching and aggregation behavior. Groups of male and female bed bugs that were unable to obtain a blood meal continued to search in the arena for the majority of the scotophase. Bed bugs that were able to obtain a blood meal returned to their shelter to aggregate 30 min after feeding. Overall, the proportion of bed bugs aggregating in shelters during the scotophase was significantly greater for those that had fed successfully than those that had not. However, all bed bugs, regardless of feeding status, began to return to shelters to aggregate 2 h prior to the photophase.

No MeSH data available.


Related in: MedlinePlus