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The Effects of Pesticides on Queen Rearing and Virus Titers in Honey Bees (Apis mellifera L.).

DeGrandi-Hoffman G, Chen Y, Simonds R - Insects (2013)

Bottom Line: Fewer queens emerged when larvae from open foraging (i.e., outside) colonies were reared in colonies fed pollen-1 or 2 compared with when those larvae were reared in outside colonies.In contrast, DWV was found in all emerged queens grafted from colonies fed pollen-1 or 2 either reared in outside hives or those fed pollen-1 or 2.The results suggest that sublethal exposure of CPF alone but especially when Pristine(®) is added reduces queen emergence possibly due to compromised immunity in developing queens.

View Article: PubMed Central - PubMed

Affiliation: Carl Hayden Bee Research Center, USDA-ARS, 2000 East Allen Road, Tucson, AZ 85719, USA. Gloria.Hoffman@ARS.USDA.GOV.

ABSTRACT
The effects of sublethal pesticide exposure on queen emergence and virus titers were examined. Queen rearing colonies were fed pollen with chlorpyrifos (CPF) alone (pollen-1) and with CPF and the fungicide Pristine(®) (pollen-2). Fewer queens emerged when larvae from open foraging (i.e., outside) colonies were reared in colonies fed pollen-1 or 2 compared with when those larvae were reared in outside colonies. Larvae grafted from and reared in colonies fed pollen-2 had lower rates of queen emergence than pollen-1 or outside colonies. Deformed wing virus (DWV) and black queen cell virus were found in nurse bees from colonies fed pollen-1 or 2 and in outside colonies. The viruses also were detected in queen larvae. However, we did not detect virus in emerged queens grafted from and reared in outside colonies. In contrast, DWV was found in all emerged queens grafted from colonies fed pollen-1 or 2 either reared in outside hives or those fed pollen-1 or 2. The results suggest that sublethal exposure of CPF alone but especially when Pristine(®) is added reduces queen emergence possibly due to compromised immunity in developing queens.

No MeSH data available.


Related in: MedlinePlus

Examples of queen larvae from unemerged queen cells. The queens are representative of those that did not emerge when reared in colonies where pollen was contaminated with chlorpyrifos alone or with added fungicide (Pristine®).
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insects-04-00071-f004: Examples of queen larvae from unemerged queen cells. The queens are representative of those that did not emerge when reared in colonies where pollen was contaminated with chlorpyrifos alone or with added fungicide (Pristine®).

Mentions: When queen cells that did not emerge in both Experiments-1 and 2 were opened, we found that either the larvae did not successfully pupate and were a black viscous mass in the cell or were fully formed dark black pupa but were dead in the cells (Figure 4).


The Effects of Pesticides on Queen Rearing and Virus Titers in Honey Bees (Apis mellifera L.).

DeGrandi-Hoffman G, Chen Y, Simonds R - Insects (2013)

Examples of queen larvae from unemerged queen cells. The queens are representative of those that did not emerge when reared in colonies where pollen was contaminated with chlorpyrifos alone or with added fungicide (Pristine®).
© Copyright Policy - open-access
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4553430&req=5

insects-04-00071-f004: Examples of queen larvae from unemerged queen cells. The queens are representative of those that did not emerge when reared in colonies where pollen was contaminated with chlorpyrifos alone or with added fungicide (Pristine®).
Mentions: When queen cells that did not emerge in both Experiments-1 and 2 were opened, we found that either the larvae did not successfully pupate and were a black viscous mass in the cell or were fully formed dark black pupa but were dead in the cells (Figure 4).

Bottom Line: Fewer queens emerged when larvae from open foraging (i.e., outside) colonies were reared in colonies fed pollen-1 or 2 compared with when those larvae were reared in outside colonies.In contrast, DWV was found in all emerged queens grafted from colonies fed pollen-1 or 2 either reared in outside hives or those fed pollen-1 or 2.The results suggest that sublethal exposure of CPF alone but especially when Pristine(®) is added reduces queen emergence possibly due to compromised immunity in developing queens.

View Article: PubMed Central - PubMed

Affiliation: Carl Hayden Bee Research Center, USDA-ARS, 2000 East Allen Road, Tucson, AZ 85719, USA. Gloria.Hoffman@ARS.USDA.GOV.

ABSTRACT
The effects of sublethal pesticide exposure on queen emergence and virus titers were examined. Queen rearing colonies were fed pollen with chlorpyrifos (CPF) alone (pollen-1) and with CPF and the fungicide Pristine(®) (pollen-2). Fewer queens emerged when larvae from open foraging (i.e., outside) colonies were reared in colonies fed pollen-1 or 2 compared with when those larvae were reared in outside colonies. Larvae grafted from and reared in colonies fed pollen-2 had lower rates of queen emergence than pollen-1 or outside colonies. Deformed wing virus (DWV) and black queen cell virus were found in nurse bees from colonies fed pollen-1 or 2 and in outside colonies. The viruses also were detected in queen larvae. However, we did not detect virus in emerged queens grafted from and reared in outside colonies. In contrast, DWV was found in all emerged queens grafted from colonies fed pollen-1 or 2 either reared in outside hives or those fed pollen-1 or 2. The results suggest that sublethal exposure of CPF alone but especially when Pristine(®) is added reduces queen emergence possibly due to compromised immunity in developing queens.

No MeSH data available.


Related in: MedlinePlus