Limits...
The Effects of Pesticides on Queen Rearing and Virus Titers in Honey Bees (Apis mellifera L.).

DeGrandi-Hoffman G, Chen Y, Simonds R - Insects (2013)

Bottom Line: Fewer queens emerged when larvae from open foraging (i.e., outside) colonies were reared in colonies fed pollen-1 or 2 compared with when those larvae were reared in outside colonies.In contrast, DWV was found in all emerged queens grafted from colonies fed pollen-1 or 2 either reared in outside hives or those fed pollen-1 or 2.The results suggest that sublethal exposure of CPF alone but especially when Pristine(®) is added reduces queen emergence possibly due to compromised immunity in developing queens.

View Article: PubMed Central - PubMed

Affiliation: Carl Hayden Bee Research Center, USDA-ARS, 2000 East Allen Road, Tucson, AZ 85719, USA. Gloria.Hoffman@ARS.USDA.GOV.

ABSTRACT
The effects of sublethal pesticide exposure on queen emergence and virus titers were examined. Queen rearing colonies were fed pollen with chlorpyrifos (CPF) alone (pollen-1) and with CPF and the fungicide Pristine(®) (pollen-2). Fewer queens emerged when larvae from open foraging (i.e., outside) colonies were reared in colonies fed pollen-1 or 2 compared with when those larvae were reared in outside colonies. Larvae grafted from and reared in colonies fed pollen-2 had lower rates of queen emergence than pollen-1 or outside colonies. Deformed wing virus (DWV) and black queen cell virus were found in nurse bees from colonies fed pollen-1 or 2 and in outside colonies. The viruses also were detected in queen larvae. However, we did not detect virus in emerged queens grafted from and reared in outside colonies. In contrast, DWV was found in all emerged queens grafted from colonies fed pollen-1 or 2 either reared in outside hives or those fed pollen-1 or 2. The results suggest that sublethal exposure of CPF alone but especially when Pristine(®) is added reduces queen emergence possibly due to compromised immunity in developing queens.

No MeSH data available.


Related in: MedlinePlus

The percentages of queen cells that were capped and queens that emerged when larvae from open foraging (“outside”) colonies were reared as queens in outside colonies or in hives in an enclosed flight area that were fed pollen with chlorpyrifos alone (pollen-1) or with added Pristine® fungicide (pollen-2). Percentages of capped queen cells and emerged queens in outside colonies were significantly higher than when the larvae were reared as queens in colonies fed pollen-1 (55% survival to capped stage; X2 = 12.3, p < 0.0001 and 40% emergence; X2 = 21.0, p ≤ 0.0001 ) or pollen-2 (76% survival to capped stage X2 = 4.03, p = 0.046; and 51.8% emergence X2 = 13.1, p < 0.0001). The percentage of larvae from outside colonies that survived to the capped brood stage in colonies fed pollen-2 was greater than in pollen-1 (76% and 55% respectively; X2 = 4.55, p = 0.03). The percentages that emerged as queens however, did not differ (pollen-1 = 40%, pollen-2 = 51.8%; X2 = 1.29, p = 0.255).
© Copyright Policy - open-access
Related In: Results  -  Collection

License
getmorefigures.php?uid=PMC4553430&req=5

insects-04-00071-f002: The percentages of queen cells that were capped and queens that emerged when larvae from open foraging (“outside”) colonies were reared as queens in outside colonies or in hives in an enclosed flight area that were fed pollen with chlorpyrifos alone (pollen-1) or with added Pristine® fungicide (pollen-2). Percentages of capped queen cells and emerged queens in outside colonies were significantly higher than when the larvae were reared as queens in colonies fed pollen-1 (55% survival to capped stage; X2 = 12.3, p < 0.0001 and 40% emergence; X2 = 21.0, p ≤ 0.0001 ) or pollen-2 (76% survival to capped stage X2 = 4.03, p = 0.046; and 51.8% emergence X2 = 13.1, p < 0.0001). The percentage of larvae from outside colonies that survived to the capped brood stage in colonies fed pollen-2 was greater than in pollen-1 (76% and 55% respectively; X2 = 4.55, p = 0.03). The percentages that emerged as queens however, did not differ (pollen-1 = 40%, pollen-2 = 51.8%; X2 = 1.29, p = 0.255).

Mentions: When larvae from outside colonies were reared into queens in outside colonies (Experiment 1), 93% survived to the capped brood stage and 93% emerged (Figure 2).These percentages were significantly higher than those when the larvae were reared into queens in colonies fed pollen-1 (55% survival to capped stage; X2 = 12.3, p < 0.0001 and 40% emergence; X2 = 21.0, p ≤ 0.0001) or pollen-2 (76% survival to capped stage X2 = 4.03, p = 0.046; and 51.8% emergence X2 = 13.1, p < 0.0001). More larvae from outside colonies survived to the capped brood stage in colonies fed pollen-2 compared with pollen-1 (76% and 55% respectively; X2 = 4.55, p = 0.03). The percentages that emerged as queens however did not differ (pollen-1 = 40%, pollen-2 = 51.8%; X2 = 1.29, p = 0.255).


The Effects of Pesticides on Queen Rearing and Virus Titers in Honey Bees (Apis mellifera L.).

DeGrandi-Hoffman G, Chen Y, Simonds R - Insects (2013)

The percentages of queen cells that were capped and queens that emerged when larvae from open foraging (“outside”) colonies were reared as queens in outside colonies or in hives in an enclosed flight area that were fed pollen with chlorpyrifos alone (pollen-1) or with added Pristine® fungicide (pollen-2). Percentages of capped queen cells and emerged queens in outside colonies were significantly higher than when the larvae were reared as queens in colonies fed pollen-1 (55% survival to capped stage; X2 = 12.3, p < 0.0001 and 40% emergence; X2 = 21.0, p ≤ 0.0001 ) or pollen-2 (76% survival to capped stage X2 = 4.03, p = 0.046; and 51.8% emergence X2 = 13.1, p < 0.0001). The percentage of larvae from outside colonies that survived to the capped brood stage in colonies fed pollen-2 was greater than in pollen-1 (76% and 55% respectively; X2 = 4.55, p = 0.03). The percentages that emerged as queens however, did not differ (pollen-1 = 40%, pollen-2 = 51.8%; X2 = 1.29, p = 0.255).
© Copyright Policy - open-access
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4553430&req=5

insects-04-00071-f002: The percentages of queen cells that were capped and queens that emerged when larvae from open foraging (“outside”) colonies were reared as queens in outside colonies or in hives in an enclosed flight area that were fed pollen with chlorpyrifos alone (pollen-1) or with added Pristine® fungicide (pollen-2). Percentages of capped queen cells and emerged queens in outside colonies were significantly higher than when the larvae were reared as queens in colonies fed pollen-1 (55% survival to capped stage; X2 = 12.3, p < 0.0001 and 40% emergence; X2 = 21.0, p ≤ 0.0001 ) or pollen-2 (76% survival to capped stage X2 = 4.03, p = 0.046; and 51.8% emergence X2 = 13.1, p < 0.0001). The percentage of larvae from outside colonies that survived to the capped brood stage in colonies fed pollen-2 was greater than in pollen-1 (76% and 55% respectively; X2 = 4.55, p = 0.03). The percentages that emerged as queens however, did not differ (pollen-1 = 40%, pollen-2 = 51.8%; X2 = 1.29, p = 0.255).
Mentions: When larvae from outside colonies were reared into queens in outside colonies (Experiment 1), 93% survived to the capped brood stage and 93% emerged (Figure 2).These percentages were significantly higher than those when the larvae were reared into queens in colonies fed pollen-1 (55% survival to capped stage; X2 = 12.3, p < 0.0001 and 40% emergence; X2 = 21.0, p ≤ 0.0001) or pollen-2 (76% survival to capped stage X2 = 4.03, p = 0.046; and 51.8% emergence X2 = 13.1, p < 0.0001). More larvae from outside colonies survived to the capped brood stage in colonies fed pollen-2 compared with pollen-1 (76% and 55% respectively; X2 = 4.55, p = 0.03). The percentages that emerged as queens however did not differ (pollen-1 = 40%, pollen-2 = 51.8%; X2 = 1.29, p = 0.255).

Bottom Line: Fewer queens emerged when larvae from open foraging (i.e., outside) colonies were reared in colonies fed pollen-1 or 2 compared with when those larvae were reared in outside colonies.In contrast, DWV was found in all emerged queens grafted from colonies fed pollen-1 or 2 either reared in outside hives or those fed pollen-1 or 2.The results suggest that sublethal exposure of CPF alone but especially when Pristine(®) is added reduces queen emergence possibly due to compromised immunity in developing queens.

View Article: PubMed Central - PubMed

Affiliation: Carl Hayden Bee Research Center, USDA-ARS, 2000 East Allen Road, Tucson, AZ 85719, USA. Gloria.Hoffman@ARS.USDA.GOV.

ABSTRACT
The effects of sublethal pesticide exposure on queen emergence and virus titers were examined. Queen rearing colonies were fed pollen with chlorpyrifos (CPF) alone (pollen-1) and with CPF and the fungicide Pristine(®) (pollen-2). Fewer queens emerged when larvae from open foraging (i.e., outside) colonies were reared in colonies fed pollen-1 or 2 compared with when those larvae were reared in outside colonies. Larvae grafted from and reared in colonies fed pollen-2 had lower rates of queen emergence than pollen-1 or outside colonies. Deformed wing virus (DWV) and black queen cell virus were found in nurse bees from colonies fed pollen-1 or 2 and in outside colonies. The viruses also were detected in queen larvae. However, we did not detect virus in emerged queens grafted from and reared in outside colonies. In contrast, DWV was found in all emerged queens grafted from colonies fed pollen-1 or 2 either reared in outside hives or those fed pollen-1 or 2. The results suggest that sublethal exposure of CPF alone but especially when Pristine(®) is added reduces queen emergence possibly due to compromised immunity in developing queens.

No MeSH data available.


Related in: MedlinePlus