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Effects of Flight on Gene Expression and Aging in the Honey Bee Brain and Flight Muscle.

Margotta JW, Mancinelli GE, Benito AA, Ammons A, Roberts SP, Elekonich MM - Insects (2012)

Bottom Line: To investigate the effects of flight, behavioral state and age on gene expression, we used whole-genome microarrays and real-time PCR.Our data suggest that the transition from behaviors requiring little to no flight (nursing) to those requiring prolonged flight bouts (foraging), rather than the amount of previous flight per se, has a major effect on gene expression.Combined with our real-time PCR data, these data suggest an epigenetic control and energy balance role in honey bee functional senescence.

View Article: PubMed Central - PubMed

Affiliation: School of Life Sciences, University of Nevada, Las Vegas, NV 89154, USA. margotta@unlv.nevada.edu.

ABSTRACT
Honey bees move through a series of in-hive tasks (e.g., "nursing") to outside tasks (e.g., "foraging") that are coincident with physiological changes and higher levels of metabolic activity. Social context can cause worker bees to speed up or slow down this process, and foragers may revert back to their earlier in-hive tasks accompanied by reversion to earlier physiological states. To investigate the effects of flight, behavioral state and age on gene expression, we used whole-genome microarrays and real-time PCR. Brain tissue and flight muscle exhibited different patterns of expression during behavioral transitions, with expression patterns in the brain reflecting both age and behavior, and expression patterns in flight muscle being primarily determined by age. Our data suggest that the transition from behaviors requiring little to no flight (nursing) to those requiring prolonged flight bouts (foraging), rather than the amount of previous flight per se, has a major effect on gene expression. Following behavioral reversion there was a partial reversion in gene expression but some aspects of forager expression patterns, such as those for genes involved in immune function, remained. Combined with our real-time PCR data, these data suggest an epigenetic control and energy balance role in honey bee functional senescence.

No MeSH data available.


Comparison of signaling pathways. The KEGG database revealed pathways and the number of differentially expressed (FDR < 0.05) transcripts. The x-axis represents the number of genes differentially expressed for the biological pathway represented on the y-axis.YN = young nurse (8–10 days-old; <1 day flight); RN = reverted nurse (25–26 days-old; 7–9 days flight); OF = old forager (25–26 days-old; 10–12 days flight); ON = older nurse (19–22 days old; <1 day flight); PF = precocious forager (8–10 days old; 2–3 days flight).
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insects-04-00009-f005: Comparison of signaling pathways. The KEGG database revealed pathways and the number of differentially expressed (FDR < 0.05) transcripts. The x-axis represents the number of genes differentially expressed for the biological pathway represented on the y-axis.YN = young nurse (8–10 days-old; <1 day flight); RN = reverted nurse (25–26 days-old; 7–9 days flight); OF = old forager (25–26 days-old; 10–12 days flight); ON = older nurse (19–22 days old; <1 day flight); PF = precocious forager (8–10 days old; 2–3 days flight).

Mentions: Along with the gene ontology analysis, we conducted a biological/biochemical pathway analysis to putatively identify pathways that differed in the expression of their constituents between age/behavioral groups (Figure 5). The analysis was implemented in ArrayTrack using the KEGG database of pathways. Similar to biological processes, young bees compared to old bees had the most differentially expressed transcripts in each pathway, while young bees compared to reverted nurse bees had far fewer differentially expressed transcripts and in some cases none at all. The oxidative phosphorylation, Jak-Stat, and Toll-like receptor pathway signaling pathways were not represented in old bees (OF and ON) compared to reverted nurse bees (RN), but pathways such as MAPK and mTOR were represented. These results suggest a number of distinct biochemical pathways are activated or repressed with age and the reversion from forager to nurse.


Effects of Flight on Gene Expression and Aging in the Honey Bee Brain and Flight Muscle.

Margotta JW, Mancinelli GE, Benito AA, Ammons A, Roberts SP, Elekonich MM - Insects (2012)

Comparison of signaling pathways. The KEGG database revealed pathways and the number of differentially expressed (FDR < 0.05) transcripts. The x-axis represents the number of genes differentially expressed for the biological pathway represented on the y-axis.YN = young nurse (8–10 days-old; <1 day flight); RN = reverted nurse (25–26 days-old; 7–9 days flight); OF = old forager (25–26 days-old; 10–12 days flight); ON = older nurse (19–22 days old; <1 day flight); PF = precocious forager (8–10 days old; 2–3 days flight).
© Copyright Policy - open-access
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4553427&req=5

insects-04-00009-f005: Comparison of signaling pathways. The KEGG database revealed pathways and the number of differentially expressed (FDR < 0.05) transcripts. The x-axis represents the number of genes differentially expressed for the biological pathway represented on the y-axis.YN = young nurse (8–10 days-old; <1 day flight); RN = reverted nurse (25–26 days-old; 7–9 days flight); OF = old forager (25–26 days-old; 10–12 days flight); ON = older nurse (19–22 days old; <1 day flight); PF = precocious forager (8–10 days old; 2–3 days flight).
Mentions: Along with the gene ontology analysis, we conducted a biological/biochemical pathway analysis to putatively identify pathways that differed in the expression of their constituents between age/behavioral groups (Figure 5). The analysis was implemented in ArrayTrack using the KEGG database of pathways. Similar to biological processes, young bees compared to old bees had the most differentially expressed transcripts in each pathway, while young bees compared to reverted nurse bees had far fewer differentially expressed transcripts and in some cases none at all. The oxidative phosphorylation, Jak-Stat, and Toll-like receptor pathway signaling pathways were not represented in old bees (OF and ON) compared to reverted nurse bees (RN), but pathways such as MAPK and mTOR were represented. These results suggest a number of distinct biochemical pathways are activated or repressed with age and the reversion from forager to nurse.

Bottom Line: To investigate the effects of flight, behavioral state and age on gene expression, we used whole-genome microarrays and real-time PCR.Our data suggest that the transition from behaviors requiring little to no flight (nursing) to those requiring prolonged flight bouts (foraging), rather than the amount of previous flight per se, has a major effect on gene expression.Combined with our real-time PCR data, these data suggest an epigenetic control and energy balance role in honey bee functional senescence.

View Article: PubMed Central - PubMed

Affiliation: School of Life Sciences, University of Nevada, Las Vegas, NV 89154, USA. margotta@unlv.nevada.edu.

ABSTRACT
Honey bees move through a series of in-hive tasks (e.g., "nursing") to outside tasks (e.g., "foraging") that are coincident with physiological changes and higher levels of metabolic activity. Social context can cause worker bees to speed up or slow down this process, and foragers may revert back to their earlier in-hive tasks accompanied by reversion to earlier physiological states. To investigate the effects of flight, behavioral state and age on gene expression, we used whole-genome microarrays and real-time PCR. Brain tissue and flight muscle exhibited different patterns of expression during behavioral transitions, with expression patterns in the brain reflecting both age and behavior, and expression patterns in flight muscle being primarily determined by age. Our data suggest that the transition from behaviors requiring little to no flight (nursing) to those requiring prolonged flight bouts (foraging), rather than the amount of previous flight per se, has a major effect on gene expression. Following behavioral reversion there was a partial reversion in gene expression but some aspects of forager expression patterns, such as those for genes involved in immune function, remained. Combined with our real-time PCR data, these data suggest an epigenetic control and energy balance role in honey bee functional senescence.

No MeSH data available.