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An engineered lipid remodeling system using a galactolipid synthase promoter during phosphate starvation enhances oil accumulation in plants.

Shimojima M, Madoka Y, Fujiwara R, Murakawa M, Yoshitake Y, Ikeda K, Koizumi R, Endo K, Ozaki K, Ohta H - Front Plant Sci (2015)

Bottom Line: Thus, the produced galactolipids are transferred to extraplastidial membranes to substitute for phospholipids.Moreover, the Arabidopsis starchless phosphoglucomutase mutant, pgm-1, accumulated higher TAG levels than did wild-type plants under Pi-depleted conditions.We generated transgenic plants that expressed a key gene involved in TAG synthesis using the Pi deficiency-responsive MGD3 promoter in wild-type and pgm-1 backgrounds.

View Article: PubMed Central - PubMed

Affiliation: Graduate School of Bioscience and Biotechnology, Tokyo Institute of Technology Yokohama, Japan.

ABSTRACT
Inorganic phosphate (Pi) depletion is a serious problem for plant growth. Membrane lipid remodeling is a defense mechanism that plants use to survive Pi-depleted conditions. During Pi starvation, phospholipids are degraded to supply Pi for other essential biological processes, whereas galactolipid synthesis in plastids is up-regulated via the transcriptional activation of monogalactosyldiacylglycerol synthase 3 (MGD3). Thus, the produced galactolipids are transferred to extraplastidial membranes to substitute for phospholipids. We found that, Pi starvation induced oil accumulation in the vegetative tissues of various seed plants without activating the transcription of enzymes involved in the later steps of triacylglycerol (TAG) biosynthesis. Moreover, the Arabidopsis starchless phosphoglucomutase mutant, pgm-1, accumulated higher TAG levels than did wild-type plants under Pi-depleted conditions. We generated transgenic plants that expressed a key gene involved in TAG synthesis using the Pi deficiency-responsive MGD3 promoter in wild-type and pgm-1 backgrounds. During Pi starvation, the transgenic plants accumulated higher TAG amounts compared with the non-transgenic plants, suggesting that the Pi deficiency-responsive promoter of galactolipid synthase in plastids may be useful for producing transgenic plants that accumulate more oil under Pi-depleted conditions.

No MeSH data available.


Related in: MedlinePlus

Growth phenotypes and fresh weight of transgenic lines under Pi-sufficient and Pi-depleted conditions. Seedlings (10 d old) of transgenic lines were transferred to MS agar containing 1% (w/v) sucrose and 0 mM (–Pi) or 1 mM (+Pi) Pi and were grown for 10 d. Growth phenotypes of (A)DGAT1_WT_3 and DGAT1_pgm-1_2, (B)DGAT2_WT_6 and DGAT2_pgm-1_2, and (C)PDAT1_WT_4 and PDAT1_pgm-1_3. Bars = 1 cm. (D) Shoot fresh weights of seedlings grown under Pi-sufficient (light gray) and Pi-depleted (dark gray) conditions. Data are the mean ± SD from three independent experinments; *P < 0.05 or **P < 0.01 for t-test vs. WT under Pi-sufficient conditions.
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Figure 5: Growth phenotypes and fresh weight of transgenic lines under Pi-sufficient and Pi-depleted conditions. Seedlings (10 d old) of transgenic lines were transferred to MS agar containing 1% (w/v) sucrose and 0 mM (–Pi) or 1 mM (+Pi) Pi and were grown for 10 d. Growth phenotypes of (A)DGAT1_WT_3 and DGAT1_pgm-1_2, (B)DGAT2_WT_6 and DGAT2_pgm-1_2, and (C)PDAT1_WT_4 and PDAT1_pgm-1_3. Bars = 1 cm. (D) Shoot fresh weights of seedlings grown under Pi-sufficient (light gray) and Pi-depleted (dark gray) conditions. Data are the mean ± SD from three independent experinments; *P < 0.05 or **P < 0.01 for t-test vs. WT under Pi-sufficient conditions.

Mentions: Under both Pi conditions, growth phenotypes and shoot fresh weights of DGAT1_WT line 3 and DGAT1_pgm-1 line 2 were similar to those of WT and pgm-1 plants (Figures 3A, 5A,D). DGAT2_WT line 6 and DGAT2_pgm-1 line 2 accumulated slightly less anthocyanin than did WT and pgm-1 plants under both Pi conditions (Figures 3A, 5B,D). The shoot fresh weight of DGAT2_WT line 6 grown under Pi-sufficient conditions was greater than those of WT and pgm-1 plants, although under Pi-depleted conditions the fresh weight was similar to those of WT and pgm-1 plants (Figure 5D). The growth phenotype of PDAT1_WT line 4 was similar to that of PDAT1_pgm-1 line 3 under both Pi conditions but differed from those of WT and pgm-1 plants (Figures 3A, 5C). Under Pi-depleted conditions, seedlings of both lines were yellowish and accumulated markedly less anthocyanin than did WT and pgm-1 plants (Figures 3A, 5C). Moreover, the fresh weight of PDAT1_pgm-1 line 3 was significantly greater than those of WT and pgm-1 plants under both Pi conditions (Figure 5D). Taken together, the shoot fresh weights of all of the transgenic plants was similar to, or higher than, those of WT and pgm-1 plants under both Pi conditions (Figure 5D).


An engineered lipid remodeling system using a galactolipid synthase promoter during phosphate starvation enhances oil accumulation in plants.

Shimojima M, Madoka Y, Fujiwara R, Murakawa M, Yoshitake Y, Ikeda K, Koizumi R, Endo K, Ozaki K, Ohta H - Front Plant Sci (2015)

Growth phenotypes and fresh weight of transgenic lines under Pi-sufficient and Pi-depleted conditions. Seedlings (10 d old) of transgenic lines were transferred to MS agar containing 1% (w/v) sucrose and 0 mM (–Pi) or 1 mM (+Pi) Pi and were grown for 10 d. Growth phenotypes of (A)DGAT1_WT_3 and DGAT1_pgm-1_2, (B)DGAT2_WT_6 and DGAT2_pgm-1_2, and (C)PDAT1_WT_4 and PDAT1_pgm-1_3. Bars = 1 cm. (D) Shoot fresh weights of seedlings grown under Pi-sufficient (light gray) and Pi-depleted (dark gray) conditions. Data are the mean ± SD from three independent experinments; *P < 0.05 or **P < 0.01 for t-test vs. WT under Pi-sufficient conditions.
© Copyright Policy
Related In: Results  -  Collection

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Show All Figures
getmorefigures.php?uid=PMC4553410&req=5

Figure 5: Growth phenotypes and fresh weight of transgenic lines under Pi-sufficient and Pi-depleted conditions. Seedlings (10 d old) of transgenic lines were transferred to MS agar containing 1% (w/v) sucrose and 0 mM (–Pi) or 1 mM (+Pi) Pi and were grown for 10 d. Growth phenotypes of (A)DGAT1_WT_3 and DGAT1_pgm-1_2, (B)DGAT2_WT_6 and DGAT2_pgm-1_2, and (C)PDAT1_WT_4 and PDAT1_pgm-1_3. Bars = 1 cm. (D) Shoot fresh weights of seedlings grown under Pi-sufficient (light gray) and Pi-depleted (dark gray) conditions. Data are the mean ± SD from three independent experinments; *P < 0.05 or **P < 0.01 for t-test vs. WT under Pi-sufficient conditions.
Mentions: Under both Pi conditions, growth phenotypes and shoot fresh weights of DGAT1_WT line 3 and DGAT1_pgm-1 line 2 were similar to those of WT and pgm-1 plants (Figures 3A, 5A,D). DGAT2_WT line 6 and DGAT2_pgm-1 line 2 accumulated slightly less anthocyanin than did WT and pgm-1 plants under both Pi conditions (Figures 3A, 5B,D). The shoot fresh weight of DGAT2_WT line 6 grown under Pi-sufficient conditions was greater than those of WT and pgm-1 plants, although under Pi-depleted conditions the fresh weight was similar to those of WT and pgm-1 plants (Figure 5D). The growth phenotype of PDAT1_WT line 4 was similar to that of PDAT1_pgm-1 line 3 under both Pi conditions but differed from those of WT and pgm-1 plants (Figures 3A, 5C). Under Pi-depleted conditions, seedlings of both lines were yellowish and accumulated markedly less anthocyanin than did WT and pgm-1 plants (Figures 3A, 5C). Moreover, the fresh weight of PDAT1_pgm-1 line 3 was significantly greater than those of WT and pgm-1 plants under both Pi conditions (Figure 5D). Taken together, the shoot fresh weights of all of the transgenic plants was similar to, or higher than, those of WT and pgm-1 plants under both Pi conditions (Figure 5D).

Bottom Line: Thus, the produced galactolipids are transferred to extraplastidial membranes to substitute for phospholipids.Moreover, the Arabidopsis starchless phosphoglucomutase mutant, pgm-1, accumulated higher TAG levels than did wild-type plants under Pi-depleted conditions.We generated transgenic plants that expressed a key gene involved in TAG synthesis using the Pi deficiency-responsive MGD3 promoter in wild-type and pgm-1 backgrounds.

View Article: PubMed Central - PubMed

Affiliation: Graduate School of Bioscience and Biotechnology, Tokyo Institute of Technology Yokohama, Japan.

ABSTRACT
Inorganic phosphate (Pi) depletion is a serious problem for plant growth. Membrane lipid remodeling is a defense mechanism that plants use to survive Pi-depleted conditions. During Pi starvation, phospholipids are degraded to supply Pi for other essential biological processes, whereas galactolipid synthesis in plastids is up-regulated via the transcriptional activation of monogalactosyldiacylglycerol synthase 3 (MGD3). Thus, the produced galactolipids are transferred to extraplastidial membranes to substitute for phospholipids. We found that, Pi starvation induced oil accumulation in the vegetative tissues of various seed plants without activating the transcription of enzymes involved in the later steps of triacylglycerol (TAG) biosynthesis. Moreover, the Arabidopsis starchless phosphoglucomutase mutant, pgm-1, accumulated higher TAG levels than did wild-type plants under Pi-depleted conditions. We generated transgenic plants that expressed a key gene involved in TAG synthesis using the Pi deficiency-responsive MGD3 promoter in wild-type and pgm-1 backgrounds. During Pi starvation, the transgenic plants accumulated higher TAG amounts compared with the non-transgenic plants, suggesting that the Pi deficiency-responsive promoter of galactolipid synthase in plastids may be useful for producing transgenic plants that accumulate more oil under Pi-depleted conditions.

No MeSH data available.


Related in: MedlinePlus