Limits...
The Inhibitory Mechanism of Gentamicin on Electrical Field Stimulation Response in Rat Bladder Smooth Muscle.

Min CH, Wang Y, Bae J, Han JH, Sohn UD - Korean J. Physiol. Pharmacol. (2015)

Bottom Line: Kinase inhibitors, such as chelerythrine (PKC inhibitor), ML-9 (MLCK inhibitor), or Y27632 (rho kinase inhibitor) were pretreated before gentamicin treatment, but did not have effect.For U73122, a phospholipase C (PLC) inhibitor however, the inhibitory effect to gentamicin was significantly attenuated in all frequencies given by the EFS.Therefore gentamicin induced inhibitory effect on EFS response in rat bladder smooth muscle was not mediated by the activation of adrenergic, cholinergic, or serotonergic receptor.

View Article: PubMed Central - PubMed

Affiliation: Department of Pharmacology, College of Pharmacy, Chung-Ang University, Seoul 156-756, Korea.

ABSTRACT
To see the inhibitory mechanism of gentamicin in response to electrical field stimulation (EFS) using the rat bladder smooth muscle, atropine or guanethidine was treated but had no effect. Methylsergide, a non-selective 5-HT1, 5-HT2 receptor antagonist was also treated but had on effect. Kinase inhibitors, such as chelerythrine (PKC inhibitor), ML-9 (MLCK inhibitor), or Y27632 (rho kinase inhibitor) were pretreated before gentamicin treatment, but did not have effect. For U73122, a phospholipase C (PLC) inhibitor however, the inhibitory effect to gentamicin was significantly attenuated in all frequencies given by the EFS. Therefore gentamicin induced inhibitory effect on EFS response in rat bladder smooth muscle was not mediated by the activation of adrenergic, cholinergic, or serotonergic receptor. The inhibition of gentamicin might be mediated through the PLC dependent pathway, but not through the PKC, MLCK or rho kinase dependent pathway.

No MeSH data available.


Effect of U73122 on EFS in rat bladder smooth muscle treated with gentamicin. U73122 (1 µM) was treated 30 min prior to EFS stimulation. For each concentration the tone was measured under the EFS of 1~8 Hz. Each point represents the mean±SE (n=8). *p<0.05, **p<0.01 vs. control.
© Copyright Policy - open-access
Related In: Results  -  Collection

License
getmorefigures.php?uid=PMC4553408&req=5

Figure 4: Effect of U73122 on EFS in rat bladder smooth muscle treated with gentamicin. U73122 (1 µM) was treated 30 min prior to EFS stimulation. For each concentration the tone was measured under the EFS of 1~8 Hz. Each point represents the mean±SE (n=8). *p<0.05, **p<0.01 vs. control.

Mentions: Fig. 4 showed the effect of U73122 (1 µM) on gentamicin induced smooth muscle relaxation. The effect of U73122 on gentamicin was significant at each frequency given by EFS as shown in the figure. For each frequency, U73122 decreased the EFS induced tone changes of rat bladder smooth muscle. Because the tone was decreased significantly when compared to that of the control, this data suggested that U73122 had a potential to block the action of gentamicin. Overall, the effect of gentamicin can be antagonized by blocking the PLC pathway (Table 1). The contraction was calculated as the data of 6 Hz that U73122 antagonized the inhibitory effect of gentamicin, but other antagonists did not.


The Inhibitory Mechanism of Gentamicin on Electrical Field Stimulation Response in Rat Bladder Smooth Muscle.

Min CH, Wang Y, Bae J, Han JH, Sohn UD - Korean J. Physiol. Pharmacol. (2015)

Effect of U73122 on EFS in rat bladder smooth muscle treated with gentamicin. U73122 (1 µM) was treated 30 min prior to EFS stimulation. For each concentration the tone was measured under the EFS of 1~8 Hz. Each point represents the mean±SE (n=8). *p<0.05, **p<0.01 vs. control.
© Copyright Policy - open-access
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4553408&req=5

Figure 4: Effect of U73122 on EFS in rat bladder smooth muscle treated with gentamicin. U73122 (1 µM) was treated 30 min prior to EFS stimulation. For each concentration the tone was measured under the EFS of 1~8 Hz. Each point represents the mean±SE (n=8). *p<0.05, **p<0.01 vs. control.
Mentions: Fig. 4 showed the effect of U73122 (1 µM) on gentamicin induced smooth muscle relaxation. The effect of U73122 on gentamicin was significant at each frequency given by EFS as shown in the figure. For each frequency, U73122 decreased the EFS induced tone changes of rat bladder smooth muscle. Because the tone was decreased significantly when compared to that of the control, this data suggested that U73122 had a potential to block the action of gentamicin. Overall, the effect of gentamicin can be antagonized by blocking the PLC pathway (Table 1). The contraction was calculated as the data of 6 Hz that U73122 antagonized the inhibitory effect of gentamicin, but other antagonists did not.

Bottom Line: Kinase inhibitors, such as chelerythrine (PKC inhibitor), ML-9 (MLCK inhibitor), or Y27632 (rho kinase inhibitor) were pretreated before gentamicin treatment, but did not have effect.For U73122, a phospholipase C (PLC) inhibitor however, the inhibitory effect to gentamicin was significantly attenuated in all frequencies given by the EFS.Therefore gentamicin induced inhibitory effect on EFS response in rat bladder smooth muscle was not mediated by the activation of adrenergic, cholinergic, or serotonergic receptor.

View Article: PubMed Central - PubMed

Affiliation: Department of Pharmacology, College of Pharmacy, Chung-Ang University, Seoul 156-756, Korea.

ABSTRACT
To see the inhibitory mechanism of gentamicin in response to electrical field stimulation (EFS) using the rat bladder smooth muscle, atropine or guanethidine was treated but had no effect. Methylsergide, a non-selective 5-HT1, 5-HT2 receptor antagonist was also treated but had on effect. Kinase inhibitors, such as chelerythrine (PKC inhibitor), ML-9 (MLCK inhibitor), or Y27632 (rho kinase inhibitor) were pretreated before gentamicin treatment, but did not have effect. For U73122, a phospholipase C (PLC) inhibitor however, the inhibitory effect to gentamicin was significantly attenuated in all frequencies given by the EFS. Therefore gentamicin induced inhibitory effect on EFS response in rat bladder smooth muscle was not mediated by the activation of adrenergic, cholinergic, or serotonergic receptor. The inhibition of gentamicin might be mediated through the PLC dependent pathway, but not through the PKC, MLCK or rho kinase dependent pathway.

No MeSH data available.