Limits...
The Inhibitory Mechanism of Gentamicin on Electrical Field Stimulation Response in Rat Bladder Smooth Muscle.

Min CH, Wang Y, Bae J, Han JH, Sohn UD - Korean J. Physiol. Pharmacol. (2015)

Bottom Line: Kinase inhibitors, such as chelerythrine (PKC inhibitor), ML-9 (MLCK inhibitor), or Y27632 (rho kinase inhibitor) were pretreated before gentamicin treatment, but did not have effect.For U73122, a phospholipase C (PLC) inhibitor however, the inhibitory effect to gentamicin was significantly attenuated in all frequencies given by the EFS.Therefore gentamicin induced inhibitory effect on EFS response in rat bladder smooth muscle was not mediated by the activation of adrenergic, cholinergic, or serotonergic receptor.

View Article: PubMed Central - PubMed

Affiliation: Department of Pharmacology, College of Pharmacy, Chung-Ang University, Seoul 156-756, Korea.

ABSTRACT
To see the inhibitory mechanism of gentamicin in response to electrical field stimulation (EFS) using the rat bladder smooth muscle, atropine or guanethidine was treated but had no effect. Methylsergide, a non-selective 5-HT1, 5-HT2 receptor antagonist was also treated but had on effect. Kinase inhibitors, such as chelerythrine (PKC inhibitor), ML-9 (MLCK inhibitor), or Y27632 (rho kinase inhibitor) were pretreated before gentamicin treatment, but did not have effect. For U73122, a phospholipase C (PLC) inhibitor however, the inhibitory effect to gentamicin was significantly attenuated in all frequencies given by the EFS. Therefore gentamicin induced inhibitory effect on EFS response in rat bladder smooth muscle was not mediated by the activation of adrenergic, cholinergic, or serotonergic receptor. The inhibition of gentamicin might be mediated through the PLC dependent pathway, but not through the PKC, MLCK or rho kinase dependent pathway.

No MeSH data available.


Effect of chelerythrine, ML-9 and Y27632 on EFS in rat bladder smooth muscle treated with gentamicin. Chelerythrine (10 µM), ML-9 (10 µM) and Y27632 (1 µM) was treated 30 min prior to EFS stimulation. The tone was measured under the EFS of 1~8 Hz. Each point represents the mean±SE (n=8).
© Copyright Policy - open-access
Related In: Results  -  Collection

License
getmorefigures.php?uid=PMC4553408&req=5

Figure 3: Effect of chelerythrine, ML-9 and Y27632 on EFS in rat bladder smooth muscle treated with gentamicin. Chelerythrine (10 µM), ML-9 (10 µM) and Y27632 (1 µM) was treated 30 min prior to EFS stimulation. The tone was measured under the EFS of 1~8 Hz. Each point represents the mean±SE (n=8).

Mentions: Fig. 3 showed the effect of different types of kinase inhibitors on gentamicin induced smooth muscle relaxation. Chelerythrine is a PKC inhibitor that is a competitive inhibitor on the substrate but a non-competitive inhibitor with respect to ATP. ML-9 is a myosin light chain kinase inhibitor that inhibits contraction by the blocking the myosin light chain phosphorylation. ML-9 is a myosin light chain kinase inhibitor that inhibits contraction by the blocking the myosin light chain phosphorylation. There was no significant effect of chelerythrine, ML-9 or Y27632 in inhibition of muscle relaxation induced by gentamicin at different frequencies. These data suggested that gentamicin was not related to the PKC, MLCK or Rho kinase dependent pathway.


The Inhibitory Mechanism of Gentamicin on Electrical Field Stimulation Response in Rat Bladder Smooth Muscle.

Min CH, Wang Y, Bae J, Han JH, Sohn UD - Korean J. Physiol. Pharmacol. (2015)

Effect of chelerythrine, ML-9 and Y27632 on EFS in rat bladder smooth muscle treated with gentamicin. Chelerythrine (10 µM), ML-9 (10 µM) and Y27632 (1 µM) was treated 30 min prior to EFS stimulation. The tone was measured under the EFS of 1~8 Hz. Each point represents the mean±SE (n=8).
© Copyright Policy - open-access
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4553408&req=5

Figure 3: Effect of chelerythrine, ML-9 and Y27632 on EFS in rat bladder smooth muscle treated with gentamicin. Chelerythrine (10 µM), ML-9 (10 µM) and Y27632 (1 µM) was treated 30 min prior to EFS stimulation. The tone was measured under the EFS of 1~8 Hz. Each point represents the mean±SE (n=8).
Mentions: Fig. 3 showed the effect of different types of kinase inhibitors on gentamicin induced smooth muscle relaxation. Chelerythrine is a PKC inhibitor that is a competitive inhibitor on the substrate but a non-competitive inhibitor with respect to ATP. ML-9 is a myosin light chain kinase inhibitor that inhibits contraction by the blocking the myosin light chain phosphorylation. ML-9 is a myosin light chain kinase inhibitor that inhibits contraction by the blocking the myosin light chain phosphorylation. There was no significant effect of chelerythrine, ML-9 or Y27632 in inhibition of muscle relaxation induced by gentamicin at different frequencies. These data suggested that gentamicin was not related to the PKC, MLCK or Rho kinase dependent pathway.

Bottom Line: Kinase inhibitors, such as chelerythrine (PKC inhibitor), ML-9 (MLCK inhibitor), or Y27632 (rho kinase inhibitor) were pretreated before gentamicin treatment, but did not have effect.For U73122, a phospholipase C (PLC) inhibitor however, the inhibitory effect to gentamicin was significantly attenuated in all frequencies given by the EFS.Therefore gentamicin induced inhibitory effect on EFS response in rat bladder smooth muscle was not mediated by the activation of adrenergic, cholinergic, or serotonergic receptor.

View Article: PubMed Central - PubMed

Affiliation: Department of Pharmacology, College of Pharmacy, Chung-Ang University, Seoul 156-756, Korea.

ABSTRACT
To see the inhibitory mechanism of gentamicin in response to electrical field stimulation (EFS) using the rat bladder smooth muscle, atropine or guanethidine was treated but had no effect. Methylsergide, a non-selective 5-HT1, 5-HT2 receptor antagonist was also treated but had on effect. Kinase inhibitors, such as chelerythrine (PKC inhibitor), ML-9 (MLCK inhibitor), or Y27632 (rho kinase inhibitor) were pretreated before gentamicin treatment, but did not have effect. For U73122, a phospholipase C (PLC) inhibitor however, the inhibitory effect to gentamicin was significantly attenuated in all frequencies given by the EFS. Therefore gentamicin induced inhibitory effect on EFS response in rat bladder smooth muscle was not mediated by the activation of adrenergic, cholinergic, or serotonergic receptor. The inhibition of gentamicin might be mediated through the PLC dependent pathway, but not through the PKC, MLCK or rho kinase dependent pathway.

No MeSH data available.