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The Inhibitory Mechanism of Gentamicin on Electrical Field Stimulation Response in Rat Bladder Smooth Muscle.

Min CH, Wang Y, Bae J, Han JH, Sohn UD - Korean J. Physiol. Pharmacol. (2015)

Bottom Line: Kinase inhibitors, such as chelerythrine (PKC inhibitor), ML-9 (MLCK inhibitor), or Y27632 (rho kinase inhibitor) were pretreated before gentamicin treatment, but did not have effect.For U73122, a phospholipase C (PLC) inhibitor however, the inhibitory effect to gentamicin was significantly attenuated in all frequencies given by the EFS.Therefore gentamicin induced inhibitory effect on EFS response in rat bladder smooth muscle was not mediated by the activation of adrenergic, cholinergic, or serotonergic receptor.

View Article: PubMed Central - PubMed

Affiliation: Department of Pharmacology, College of Pharmacy, Chung-Ang University, Seoul 156-756, Korea.

ABSTRACT
To see the inhibitory mechanism of gentamicin in response to electrical field stimulation (EFS) using the rat bladder smooth muscle, atropine or guanethidine was treated but had no effect. Methylsergide, a non-selective 5-HT1, 5-HT2 receptor antagonist was also treated but had on effect. Kinase inhibitors, such as chelerythrine (PKC inhibitor), ML-9 (MLCK inhibitor), or Y27632 (rho kinase inhibitor) were pretreated before gentamicin treatment, but did not have effect. For U73122, a phospholipase C (PLC) inhibitor however, the inhibitory effect to gentamicin was significantly attenuated in all frequencies given by the EFS. Therefore gentamicin induced inhibitory effect on EFS response in rat bladder smooth muscle was not mediated by the activation of adrenergic, cholinergic, or serotonergic receptor. The inhibition of gentamicin might be mediated through the PLC dependent pathway, but not through the PKC, MLCK or rho kinase dependent pathway.

No MeSH data available.


Effect of atropine, guanethidine and methylsergide on EFS in rat bladder smooth muscle treated with gentamicin. Atropine (0.1 mM), guanethidine (0.1 mM) or rmethylsergide (10 µM) was treated 30 min prior to EFS stimulation. The tone was measured under the EFS of 1~8 Hz. Each point represents the mean±SE (n=8).
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Figure 2: Effect of atropine, guanethidine and methylsergide on EFS in rat bladder smooth muscle treated with gentamicin. Atropine (0.1 mM), guanethidine (0.1 mM) or rmethylsergide (10 µM) was treated 30 min prior to EFS stimulation. The tone was measured under the EFS of 1~8 Hz. Each point represents the mean±SE (n=8).

Mentions: Fig. 2 showed the effect of receptor blockers on gentamicin induced smooth muscle relaxation. To test whether cholinergic, adrenergic or serotonergic effect is implicated in the inhibitory effect of gentamicin on muscle relaxation, atropine (0.1 mM), guanethidine (0.1 mM) and methylsergide (10 µM) was pretreated 30 min before the experiment. Atropine is a central and peripheral muscarinic cholinergic receptors antagonist, and guanethidine is an antihypertensive agent that inhibits selective transmission in post-ganglionic adrenergic nerves, and methylsergide is a non-selective 5-HT1, 5-HT2 receptor antagonist. Atropine, guanethidine and methylsergide did not affect the gentamicin-induced inhibitory effect on muscle relaxation at different frequencies. Therefore gentamicin induced muscle relaxation on EFS response in rat bladder smooth muscle is not mediated by the activation of adrenergic, cholinergic and serotonergic receptor.


The Inhibitory Mechanism of Gentamicin on Electrical Field Stimulation Response in Rat Bladder Smooth Muscle.

Min CH, Wang Y, Bae J, Han JH, Sohn UD - Korean J. Physiol. Pharmacol. (2015)

Effect of atropine, guanethidine and methylsergide on EFS in rat bladder smooth muscle treated with gentamicin. Atropine (0.1 mM), guanethidine (0.1 mM) or rmethylsergide (10 µM) was treated 30 min prior to EFS stimulation. The tone was measured under the EFS of 1~8 Hz. Each point represents the mean±SE (n=8).
© Copyright Policy - open-access
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4553408&req=5

Figure 2: Effect of atropine, guanethidine and methylsergide on EFS in rat bladder smooth muscle treated with gentamicin. Atropine (0.1 mM), guanethidine (0.1 mM) or rmethylsergide (10 µM) was treated 30 min prior to EFS stimulation. The tone was measured under the EFS of 1~8 Hz. Each point represents the mean±SE (n=8).
Mentions: Fig. 2 showed the effect of receptor blockers on gentamicin induced smooth muscle relaxation. To test whether cholinergic, adrenergic or serotonergic effect is implicated in the inhibitory effect of gentamicin on muscle relaxation, atropine (0.1 mM), guanethidine (0.1 mM) and methylsergide (10 µM) was pretreated 30 min before the experiment. Atropine is a central and peripheral muscarinic cholinergic receptors antagonist, and guanethidine is an antihypertensive agent that inhibits selective transmission in post-ganglionic adrenergic nerves, and methylsergide is a non-selective 5-HT1, 5-HT2 receptor antagonist. Atropine, guanethidine and methylsergide did not affect the gentamicin-induced inhibitory effect on muscle relaxation at different frequencies. Therefore gentamicin induced muscle relaxation on EFS response in rat bladder smooth muscle is not mediated by the activation of adrenergic, cholinergic and serotonergic receptor.

Bottom Line: Kinase inhibitors, such as chelerythrine (PKC inhibitor), ML-9 (MLCK inhibitor), or Y27632 (rho kinase inhibitor) were pretreated before gentamicin treatment, but did not have effect.For U73122, a phospholipase C (PLC) inhibitor however, the inhibitory effect to gentamicin was significantly attenuated in all frequencies given by the EFS.Therefore gentamicin induced inhibitory effect on EFS response in rat bladder smooth muscle was not mediated by the activation of adrenergic, cholinergic, or serotonergic receptor.

View Article: PubMed Central - PubMed

Affiliation: Department of Pharmacology, College of Pharmacy, Chung-Ang University, Seoul 156-756, Korea.

ABSTRACT
To see the inhibitory mechanism of gentamicin in response to electrical field stimulation (EFS) using the rat bladder smooth muscle, atropine or guanethidine was treated but had no effect. Methylsergide, a non-selective 5-HT1, 5-HT2 receptor antagonist was also treated but had on effect. Kinase inhibitors, such as chelerythrine (PKC inhibitor), ML-9 (MLCK inhibitor), or Y27632 (rho kinase inhibitor) were pretreated before gentamicin treatment, but did not have effect. For U73122, a phospholipase C (PLC) inhibitor however, the inhibitory effect to gentamicin was significantly attenuated in all frequencies given by the EFS. Therefore gentamicin induced inhibitory effect on EFS response in rat bladder smooth muscle was not mediated by the activation of adrenergic, cholinergic, or serotonergic receptor. The inhibition of gentamicin might be mediated through the PLC dependent pathway, but not through the PKC, MLCK or rho kinase dependent pathway.

No MeSH data available.