Limits...
The Inhibitory Mechanism of Gentamicin on Electrical Field Stimulation Response in Rat Bladder Smooth Muscle.

Min CH, Wang Y, Bae J, Han JH, Sohn UD - Korean J. Physiol. Pharmacol. (2015)

Bottom Line: Kinase inhibitors, such as chelerythrine (PKC inhibitor), ML-9 (MLCK inhibitor), or Y27632 (rho kinase inhibitor) were pretreated before gentamicin treatment, but did not have effect.For U73122, a phospholipase C (PLC) inhibitor however, the inhibitory effect to gentamicin was significantly attenuated in all frequencies given by the EFS.Therefore gentamicin induced inhibitory effect on EFS response in rat bladder smooth muscle was not mediated by the activation of adrenergic, cholinergic, or serotonergic receptor.

View Article: PubMed Central - PubMed

Affiliation: Department of Pharmacology, College of Pharmacy, Chung-Ang University, Seoul 156-756, Korea.

ABSTRACT
To see the inhibitory mechanism of gentamicin in response to electrical field stimulation (EFS) using the rat bladder smooth muscle, atropine or guanethidine was treated but had no effect. Methylsergide, a non-selective 5-HT1, 5-HT2 receptor antagonist was also treated but had on effect. Kinase inhibitors, such as chelerythrine (PKC inhibitor), ML-9 (MLCK inhibitor), or Y27632 (rho kinase inhibitor) were pretreated before gentamicin treatment, but did not have effect. For U73122, a phospholipase C (PLC) inhibitor however, the inhibitory effect to gentamicin was significantly attenuated in all frequencies given by the EFS. Therefore gentamicin induced inhibitory effect on EFS response in rat bladder smooth muscle was not mediated by the activation of adrenergic, cholinergic, or serotonergic receptor. The inhibition of gentamicin might be mediated through the PLC dependent pathway, but not through the PKC, MLCK or rho kinase dependent pathway.

No MeSH data available.


Representative tracing showing the effect of gentamycin on EFS in the rat bladder smooth muscle. Gentamicin was added cumulatively with an interval of 5 min. The tone was measured under the EFS of 1, 2, 4, 6, 8 Hz. Each point represents the mean±SE (n=8).
© Copyright Policy - open-access
Related In: Results  -  Collection

License
getmorefigures.php?uid=PMC4553408&req=5

Figure 1: Representative tracing showing the effect of gentamycin on EFS in the rat bladder smooth muscle. Gentamicin was added cumulatively with an interval of 5 min. The tone was measured under the EFS of 1, 2, 4, 6, 8 Hz. Each point represents the mean±SE (n=8).

Mentions: The muscle strips isolated from the rat bladder did not display a spontaneous phasic activity, and was rather tranquil. The strips were stimulated with pulse trains of 40 V in amplitude and 10 seconds in duration, with pulse duration of 1 millisecond at the frequency at 1, 2, 4, 6, 8 Hz respectively. To test the effect of gentamicin on bladder smooth muscle, gentamicin 0.1 mM was treated 5 min before the experiment. The contractile response to EFS was decreased after treatment with gentamicin (Fig. 1A). To see the dose dependent effect of gentamicin in detail, middle doses such as 3 µM, 10 µM and 30 µM were also measured (Fig. 1B). The tone change was measured by the difference of the increased tone to the basal tone. As the dosage and frequency of EFS increased, the relaxing effect of gentamicin increased dependently. A significant decrease in the tone change appeared only in the highest dose, 0.1 mM at all frequencies measured.


The Inhibitory Mechanism of Gentamicin on Electrical Field Stimulation Response in Rat Bladder Smooth Muscle.

Min CH, Wang Y, Bae J, Han JH, Sohn UD - Korean J. Physiol. Pharmacol. (2015)

Representative tracing showing the effect of gentamycin on EFS in the rat bladder smooth muscle. Gentamicin was added cumulatively with an interval of 5 min. The tone was measured under the EFS of 1, 2, 4, 6, 8 Hz. Each point represents the mean±SE (n=8).
© Copyright Policy - open-access
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4553408&req=5

Figure 1: Representative tracing showing the effect of gentamycin on EFS in the rat bladder smooth muscle. Gentamicin was added cumulatively with an interval of 5 min. The tone was measured under the EFS of 1, 2, 4, 6, 8 Hz. Each point represents the mean±SE (n=8).
Mentions: The muscle strips isolated from the rat bladder did not display a spontaneous phasic activity, and was rather tranquil. The strips were stimulated with pulse trains of 40 V in amplitude and 10 seconds in duration, with pulse duration of 1 millisecond at the frequency at 1, 2, 4, 6, 8 Hz respectively. To test the effect of gentamicin on bladder smooth muscle, gentamicin 0.1 mM was treated 5 min before the experiment. The contractile response to EFS was decreased after treatment with gentamicin (Fig. 1A). To see the dose dependent effect of gentamicin in detail, middle doses such as 3 µM, 10 µM and 30 µM were also measured (Fig. 1B). The tone change was measured by the difference of the increased tone to the basal tone. As the dosage and frequency of EFS increased, the relaxing effect of gentamicin increased dependently. A significant decrease in the tone change appeared only in the highest dose, 0.1 mM at all frequencies measured.

Bottom Line: Kinase inhibitors, such as chelerythrine (PKC inhibitor), ML-9 (MLCK inhibitor), or Y27632 (rho kinase inhibitor) were pretreated before gentamicin treatment, but did not have effect.For U73122, a phospholipase C (PLC) inhibitor however, the inhibitory effect to gentamicin was significantly attenuated in all frequencies given by the EFS.Therefore gentamicin induced inhibitory effect on EFS response in rat bladder smooth muscle was not mediated by the activation of adrenergic, cholinergic, or serotonergic receptor.

View Article: PubMed Central - PubMed

Affiliation: Department of Pharmacology, College of Pharmacy, Chung-Ang University, Seoul 156-756, Korea.

ABSTRACT
To see the inhibitory mechanism of gentamicin in response to electrical field stimulation (EFS) using the rat bladder smooth muscle, atropine or guanethidine was treated but had no effect. Methylsergide, a non-selective 5-HT1, 5-HT2 receptor antagonist was also treated but had on effect. Kinase inhibitors, such as chelerythrine (PKC inhibitor), ML-9 (MLCK inhibitor), or Y27632 (rho kinase inhibitor) were pretreated before gentamicin treatment, but did not have effect. For U73122, a phospholipase C (PLC) inhibitor however, the inhibitory effect to gentamicin was significantly attenuated in all frequencies given by the EFS. Therefore gentamicin induced inhibitory effect on EFS response in rat bladder smooth muscle was not mediated by the activation of adrenergic, cholinergic, or serotonergic receptor. The inhibition of gentamicin might be mediated through the PLC dependent pathway, but not through the PKC, MLCK or rho kinase dependent pathway.

No MeSH data available.