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A Higher Angiogenin Expression is Associated With a Nonnuclear Maspin Location in Laryngeal Carcinoma.

Lovato A, Lionello M, Staffieri A, Blandamura S, Tealdo G, Giacomelli L, Staffieri C, Marioni G - Clin Exp Otorhinolaryngol (2015)

Bottom Line: The mean ANG expression was significantly higher in cases with loco-regional recurrent disease (P=0.007); and patients with an ANG expression ≥5.0% had a significantly shorter DFS than those with an ANG expression <5.0% (P=0.007).On multivariate analysis, ANG expression ≥5.0% was a significant, independent, negative prognostic factor in terms of DFS (P=0.041).Our results support the hypothesis that a higher ANG expression is associated with a nonnuclear Maspin expression pattern in patients with LSCC.

View Article: PubMed Central - PubMed

Affiliation: Department of Neurosciences, Otolaryngology Section, University of Padova, Padova, Italy.

ABSTRACT

Objectives: In numerous malignancies, angiogenin (ANG) and Maspin are important proangiogenic and antiangiogenic regulators, respectively. The aim of this study was to identify potential relationships between the biological roles of these two proteins in laryngeal squamous cell carcinoma (LSCC).

Methods: Immunohistochemical staining for ANG and Maspin was performed on specimens from 76 consecutive LSCC patients treated with surgery alone, considering the subcellular pattern of Maspin expression. Univariate and multivariate statistical models were used for prognostic purposes.

Results: On univariate analysis, a different level of ANG expression was seen for patients stratified by subcellular Maspin expression pattern: the mean ANG expression was higher in cases with a nonnuclear MASPIN expression than in those with a nuclear pattern (P=0.002). Disease-free survival (DFS; in months) differed significantly when patients were stratified by N stage (P=0.01). Patients whose Maspin expression was nonnuclear (i.e., it was cytoplasmic or there was none) had a significantly higher recurrence rate (P<0.001), and shorter DFS (P=0.01) than those with a nuclear Maspin pattern. The mean ANG expression was significantly higher in cases with loco-regional recurrent disease (P=0.007); and patients with an ANG expression ≥5.0% had a significantly shorter DFS than those with an ANG expression <5.0% (P=0.007). On multivariate analysis, ANG expression ≥5.0% was a significant, independent, negative prognostic factor in terms of DFS (P=0.041).

Conclusion: Our results support the hypothesis that a higher ANG expression is associated with a nonnuclear Maspin expression pattern in patients with LSCC. Further studies are needed to clarify the relationship between the ANG and Maspin pathways, and their potential diagnostic and therapeutic role in LSCC.

No MeSH data available.


Related in: MedlinePlus

(A, B) A patient whose laryngeal squamous cell carcinoma recurred, with a high angiogenin (ANG) expression (A), and a cytoplasmic pattern of Maspin expression (B), in carcinoma cells; (C, D) a patient who had no evidence of disease during the follow-up, with a low ANG expression (C), and a nuclear pattern of Maspin expression (D). (A-D) Immunohistochemical staining.
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Figure 1: (A, B) A patient whose laryngeal squamous cell carcinoma recurred, with a high angiogenin (ANG) expression (A), and a cytoplasmic pattern of Maspin expression (B), in carcinoma cells; (C, D) a patient who had no evidence of disease during the follow-up, with a low ANG expression (C), and a nuclear pattern of Maspin expression (D). (A-D) Immunohistochemical staining.

Mentions: ANG staining in laryngeal carcinoma cells was seen in 51 of 76 carcinoma samples (67%) (Table 1). The carcinoma cells showed prominent ANG staining of variable intensity in the membrane and cytoplasm (Fig. 1A, C). Statistical analysis failed to identify any significant associations between ANG expression and pT, N (pN+ vs. cN0 or pN0), stage, or grade (Mann-Whitney U-test, P=0.25, P=0.40, P=0.77, and P=0.19, respectively).


A Higher Angiogenin Expression is Associated With a Nonnuclear Maspin Location in Laryngeal Carcinoma.

Lovato A, Lionello M, Staffieri A, Blandamura S, Tealdo G, Giacomelli L, Staffieri C, Marioni G - Clin Exp Otorhinolaryngol (2015)

(A, B) A patient whose laryngeal squamous cell carcinoma recurred, with a high angiogenin (ANG) expression (A), and a cytoplasmic pattern of Maspin expression (B), in carcinoma cells; (C, D) a patient who had no evidence of disease during the follow-up, with a low ANG expression (C), and a nuclear pattern of Maspin expression (D). (A-D) Immunohistochemical staining.
© Copyright Policy - open-access
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4553359&req=5

Figure 1: (A, B) A patient whose laryngeal squamous cell carcinoma recurred, with a high angiogenin (ANG) expression (A), and a cytoplasmic pattern of Maspin expression (B), in carcinoma cells; (C, D) a patient who had no evidence of disease during the follow-up, with a low ANG expression (C), and a nuclear pattern of Maspin expression (D). (A-D) Immunohistochemical staining.
Mentions: ANG staining in laryngeal carcinoma cells was seen in 51 of 76 carcinoma samples (67%) (Table 1). The carcinoma cells showed prominent ANG staining of variable intensity in the membrane and cytoplasm (Fig. 1A, C). Statistical analysis failed to identify any significant associations between ANG expression and pT, N (pN+ vs. cN0 or pN0), stage, or grade (Mann-Whitney U-test, P=0.25, P=0.40, P=0.77, and P=0.19, respectively).

Bottom Line: The mean ANG expression was significantly higher in cases with loco-regional recurrent disease (P=0.007); and patients with an ANG expression ≥5.0% had a significantly shorter DFS than those with an ANG expression <5.0% (P=0.007).On multivariate analysis, ANG expression ≥5.0% was a significant, independent, negative prognostic factor in terms of DFS (P=0.041).Our results support the hypothesis that a higher ANG expression is associated with a nonnuclear Maspin expression pattern in patients with LSCC.

View Article: PubMed Central - PubMed

Affiliation: Department of Neurosciences, Otolaryngology Section, University of Padova, Padova, Italy.

ABSTRACT

Objectives: In numerous malignancies, angiogenin (ANG) and Maspin are important proangiogenic and antiangiogenic regulators, respectively. The aim of this study was to identify potential relationships between the biological roles of these two proteins in laryngeal squamous cell carcinoma (LSCC).

Methods: Immunohistochemical staining for ANG and Maspin was performed on specimens from 76 consecutive LSCC patients treated with surgery alone, considering the subcellular pattern of Maspin expression. Univariate and multivariate statistical models were used for prognostic purposes.

Results: On univariate analysis, a different level of ANG expression was seen for patients stratified by subcellular Maspin expression pattern: the mean ANG expression was higher in cases with a nonnuclear MASPIN expression than in those with a nuclear pattern (P=0.002). Disease-free survival (DFS; in months) differed significantly when patients were stratified by N stage (P=0.01). Patients whose Maspin expression was nonnuclear (i.e., it was cytoplasmic or there was none) had a significantly higher recurrence rate (P<0.001), and shorter DFS (P=0.01) than those with a nuclear Maspin pattern. The mean ANG expression was significantly higher in cases with loco-regional recurrent disease (P=0.007); and patients with an ANG expression ≥5.0% had a significantly shorter DFS than those with an ANG expression <5.0% (P=0.007). On multivariate analysis, ANG expression ≥5.0% was a significant, independent, negative prognostic factor in terms of DFS (P=0.041).

Conclusion: Our results support the hypothesis that a higher ANG expression is associated with a nonnuclear Maspin expression pattern in patients with LSCC. Further studies are needed to clarify the relationship between the ANG and Maspin pathways, and their potential diagnostic and therapeutic role in LSCC.

No MeSH data available.


Related in: MedlinePlus