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Antiallergic Effects of Trichostatin A in a Murine Model of Allergic Rhinitis.

Cho JS, Kang JH, Han IH, Um JY, Park IH, Lee SH, Lee HM - Clin Exp Otorhinolaryngol (2015)

Bottom Line: TSA reduced the scores of allergic nasal symptoms and the amount of eosinophil infiltration into the nasal mucosa.TSA suppressed OVA-specific IgE levels and reduced expression of the IL-4 and IL-5.The levels of Foxp3, IL-10, and TGF-β were increased in pretreatment with TSA as compared to control group.

View Article: PubMed Central - PubMed

Affiliation: Brain Korea 21 Plus for Biomedical Science, Seoul, Korea. ; Institute for Medical Devices Clinical Trial Center, Seoul, Korea.

ABSTRACT

Objectives: Trichostatin A (TSA), an inhibitor of histone deacetylase, has been shown to play an important role in attenuating asthmatic inflammation. However, the effect of TSA in allergic rhinitis is not known. The aims of this study were to investigate the effect of TSA on allergic nasal inflammation and on the induction of regulatory T cells in a murine model of allergic rhinitis.

Methods: BALB/c mice were sensitized intraperitoneally with ovalbumin (OVA) and then challenged intranasally with OVA. TSA (1 mg/kg) was given to the treatment group, and multiple parameters of allergic responses were evaluated to determine the effects of TSA on allergic rhinitis. Allergic nasal symptom scores, including frequency of rubbing and sneezing, were checked. Eosinophil infiltrations were stained with Chromotrope 2R, and the expression levels of OVA-specific IgE, T-helper 1 (Th1) cytokine (interferon-gamma [IFN-γ]), Th2 cytokines (interleukin [IL] 4 and IL-5) and Treg (Foxp3, IL-10, and transforming growth factor-beta [TGF-β]) were measured by quantitative reverse transcription-polymerase chain reaction or enzyme-linked immunosorbent assay.

Results: TSA reduced the scores of allergic nasal symptoms and the amount of eosinophil infiltration into the nasal mucosa. TSA suppressed OVA-specific IgE levels and reduced expression of the IL-4 and IL-5. However, the expression of IFN-γ was unchanged in the treatment group. The levels of Foxp3, IL-10, and TGF-β were increased in pretreatment with TSA as compared to control group.

Conclusion: This study shows that TSA induced antiallergic effects by decreasing eosinophilic infiltration and Th2 cytokines in a murine model of allergic rhinitis via regulation of Tregs. Thus, TSA may be considered a potentially therapeutic agent in treating allergic rhinitis.

No MeSH data available.


Related in: MedlinePlus

Nasal symptom scores. (A) Rubbing score. (B) Sneezing score. OVA, ovalbumin; TSA, trichostatin A. *P<0.05 and **P<0.01 compared to control. †P<0.05 compared to OVA.
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Figure 2: Nasal symptom scores. (A) Rubbing score. (B) Sneezing score. OVA, ovalbumin; TSA, trichostatin A. *P<0.05 and **P<0.01 compared to control. †P<0.05 compared to OVA.

Mentions: The frequency of nasal rubbing and sneezing increased significantly in the OVA treatment group versus the control group. However, the OVA+TSA group exhibited a significant decrease in the frequency of nasal rubbing and sneezing compared to the group undergoing treatment with OVA only (Fig. 2), demonstrating that TSA inhibits OVA-induced allergic nasal symptoms.


Antiallergic Effects of Trichostatin A in a Murine Model of Allergic Rhinitis.

Cho JS, Kang JH, Han IH, Um JY, Park IH, Lee SH, Lee HM - Clin Exp Otorhinolaryngol (2015)

Nasal symptom scores. (A) Rubbing score. (B) Sneezing score. OVA, ovalbumin; TSA, trichostatin A. *P<0.05 and **P<0.01 compared to control. †P<0.05 compared to OVA.
© Copyright Policy - open-access
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4553355&req=5

Figure 2: Nasal symptom scores. (A) Rubbing score. (B) Sneezing score. OVA, ovalbumin; TSA, trichostatin A. *P<0.05 and **P<0.01 compared to control. †P<0.05 compared to OVA.
Mentions: The frequency of nasal rubbing and sneezing increased significantly in the OVA treatment group versus the control group. However, the OVA+TSA group exhibited a significant decrease in the frequency of nasal rubbing and sneezing compared to the group undergoing treatment with OVA only (Fig. 2), demonstrating that TSA inhibits OVA-induced allergic nasal symptoms.

Bottom Line: TSA reduced the scores of allergic nasal symptoms and the amount of eosinophil infiltration into the nasal mucosa.TSA suppressed OVA-specific IgE levels and reduced expression of the IL-4 and IL-5.The levels of Foxp3, IL-10, and TGF-β were increased in pretreatment with TSA as compared to control group.

View Article: PubMed Central - PubMed

Affiliation: Brain Korea 21 Plus for Biomedical Science, Seoul, Korea. ; Institute for Medical Devices Clinical Trial Center, Seoul, Korea.

ABSTRACT

Objectives: Trichostatin A (TSA), an inhibitor of histone deacetylase, has been shown to play an important role in attenuating asthmatic inflammation. However, the effect of TSA in allergic rhinitis is not known. The aims of this study were to investigate the effect of TSA on allergic nasal inflammation and on the induction of regulatory T cells in a murine model of allergic rhinitis.

Methods: BALB/c mice were sensitized intraperitoneally with ovalbumin (OVA) and then challenged intranasally with OVA. TSA (1 mg/kg) was given to the treatment group, and multiple parameters of allergic responses were evaluated to determine the effects of TSA on allergic rhinitis. Allergic nasal symptom scores, including frequency of rubbing and sneezing, were checked. Eosinophil infiltrations were stained with Chromotrope 2R, and the expression levels of OVA-specific IgE, T-helper 1 (Th1) cytokine (interferon-gamma [IFN-γ]), Th2 cytokines (interleukin [IL] 4 and IL-5) and Treg (Foxp3, IL-10, and transforming growth factor-beta [TGF-β]) were measured by quantitative reverse transcription-polymerase chain reaction or enzyme-linked immunosorbent assay.

Results: TSA reduced the scores of allergic nasal symptoms and the amount of eosinophil infiltration into the nasal mucosa. TSA suppressed OVA-specific IgE levels and reduced expression of the IL-4 and IL-5. However, the expression of IFN-γ was unchanged in the treatment group. The levels of Foxp3, IL-10, and TGF-β were increased in pretreatment with TSA as compared to control group.

Conclusion: This study shows that TSA induced antiallergic effects by decreasing eosinophilic infiltration and Th2 cytokines in a murine model of allergic rhinitis via regulation of Tregs. Thus, TSA may be considered a potentially therapeutic agent in treating allergic rhinitis.

No MeSH data available.


Related in: MedlinePlus