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Intravenous Cardiac Stem Cell-Derived Exosomes Ameliorate Cardiac Dysfunction in Doxorubicin Induced Dilated Cardiomyopathy.

Vandergriff AC, de Andrade JB, Tang J, Hensley MT, Piedrahita JA, Caranasos TG, Cheng K - Stem Cells Int (2015)

Bottom Line: CSC-derived exosomes (CSC-XOs) have been shown to be responsible for a large portion of the regenerative effects of CSCs.Mice receiving CSC-XOs showed improved heart function via echocardiography, as well as decreased apoptosis and fibrosis.The use of CSC-XOs holds promise for overcoming the limitations of stem cells and improving cardiac therapies.

View Article: PubMed Central - PubMed

Affiliation: Molecular Biomedical Sciences, College of Veterinary Medicine, North Carolina State University, Raleigh, NC 27695, USA ; Joint Department of Biomedical Engineering, UNC-Chapel Hill and NC State University, Chapel Hill and Raleigh, NC, USA.

ABSTRACT
Despite the efficacy of cardiac stem cells (CSCs) for treatment of cardiomyopathies, there are many limitations to stem cell therapies. CSC-derived exosomes (CSC-XOs) have been shown to be responsible for a large portion of the regenerative effects of CSCs. Using a mouse model of doxorubicin induced dilated cardiomyopathy, we study the effects of systemic delivery of human CSC-XOs in mice. Mice receiving CSC-XOs showed improved heart function via echocardiography, as well as decreased apoptosis and fibrosis. In spite of using immunocompetent mice and human CSC-XOs, mice showed no adverse immune reaction. The use of CSC-XOs holds promise for overcoming the limitations of stem cells and improving cardiac therapies.

No MeSH data available.


Related in: MedlinePlus

H&E staining of heart sections. Lack of cellular infiltrations indicates no negative immune response by mice.
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fig8: H&E staining of heart sections. Lack of cellular infiltrations indicates no negative immune response by mice.

Mentions: Using H&E staining, there was little infiltration shown in animals receiving human exosomes compared to the control group (Figure 8), suggesting CSC-XO did not elicit measurable immune response. A lack of immune response from xenogeneic translation indicates that using allogeneic transplantation in humans is highly probable. Despite the lack of immune response in the heart, other organs were not examined for any immune response. Future studies are needed to examine other organs and systemic immune response to allogeneic exosome therapies. Nevertheless, if allogeneic transplantation is safe, it allows for exosomes to be collected from controlled cell lines that are known to stably produce therapeutic exosomes, as well as allowing for the exosomes to be pulled “out of the shelf” for acute cardiac events.


Intravenous Cardiac Stem Cell-Derived Exosomes Ameliorate Cardiac Dysfunction in Doxorubicin Induced Dilated Cardiomyopathy.

Vandergriff AC, de Andrade JB, Tang J, Hensley MT, Piedrahita JA, Caranasos TG, Cheng K - Stem Cells Int (2015)

H&E staining of heart sections. Lack of cellular infiltrations indicates no negative immune response by mice.
© Copyright Policy - open-access
Related In: Results  -  Collection

Show All Figures
getmorefigures.php?uid=PMC4553317&req=5

fig8: H&E staining of heart sections. Lack of cellular infiltrations indicates no negative immune response by mice.
Mentions: Using H&E staining, there was little infiltration shown in animals receiving human exosomes compared to the control group (Figure 8), suggesting CSC-XO did not elicit measurable immune response. A lack of immune response from xenogeneic translation indicates that using allogeneic transplantation in humans is highly probable. Despite the lack of immune response in the heart, other organs were not examined for any immune response. Future studies are needed to examine other organs and systemic immune response to allogeneic exosome therapies. Nevertheless, if allogeneic transplantation is safe, it allows for exosomes to be collected from controlled cell lines that are known to stably produce therapeutic exosomes, as well as allowing for the exosomes to be pulled “out of the shelf” for acute cardiac events.

Bottom Line: CSC-derived exosomes (CSC-XOs) have been shown to be responsible for a large portion of the regenerative effects of CSCs.Mice receiving CSC-XOs showed improved heart function via echocardiography, as well as decreased apoptosis and fibrosis.The use of CSC-XOs holds promise for overcoming the limitations of stem cells and improving cardiac therapies.

View Article: PubMed Central - PubMed

Affiliation: Molecular Biomedical Sciences, College of Veterinary Medicine, North Carolina State University, Raleigh, NC 27695, USA ; Joint Department of Biomedical Engineering, UNC-Chapel Hill and NC State University, Chapel Hill and Raleigh, NC, USA.

ABSTRACT
Despite the efficacy of cardiac stem cells (CSCs) for treatment of cardiomyopathies, there are many limitations to stem cell therapies. CSC-derived exosomes (CSC-XOs) have been shown to be responsible for a large portion of the regenerative effects of CSCs. Using a mouse model of doxorubicin induced dilated cardiomyopathy, we study the effects of systemic delivery of human CSC-XOs in mice. Mice receiving CSC-XOs showed improved heart function via echocardiography, as well as decreased apoptosis and fibrosis. In spite of using immunocompetent mice and human CSC-XOs, mice showed no adverse immune reaction. The use of CSC-XOs holds promise for overcoming the limitations of stem cells and improving cardiac therapies.

No MeSH data available.


Related in: MedlinePlus