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Effect of Ethanol/Water Solvents on Phenolic Profiles and Antioxidant Properties of Beijing Propolis Extracts.

Sun C, Wu Z, Wang Z, Zhang H - Evid Based Complement Alternat Med (2015)

Bottom Line: Our results reveal that phenolic compounds and antioxidant properties of propolis extracts were significantly dependent on the concentration of ethanol/water solvents.In particular, 75 wt.% ethanol/water solvent may be the best for the highest extraction yield and the strongest antioxidant properties.Caffeic acid, benzyl caffeate, phenethyl caffeate, 5-methoxy pinobanksin, pinobanksin, pinocembrin, pinobanksin-3-O-acetate, chrysin, and galangin were the characteristic compounds of Beijing propolis, and these compounds seem to verify that Beijing propolis may be poplar-type propolis.

View Article: PubMed Central - PubMed

Affiliation: Institute of Apicultural Research, Chinese Academy of Agricultural Sciences, Beijing 100093, China.

ABSTRACT
Propolis is a natural substance known to be beneficial for human health and used as a folk medicine in many parts of the world. In this study, phenolic profiles and antioxidant properties of Beijing propolis extracted by different ethanol/water solvents were analyzed. Our results reveal that phenolic compounds and antioxidant properties of propolis extracts were significantly dependent on the concentration of ethanol/water solvents. Totally, 29 phenolic compounds were identified: 12 phenolic acids, 13 flavonoids, and 4 phenolic acid esters. In particular, 75 wt.% ethanol/water solvent may be the best for the highest extraction yield and the strongest antioxidant properties. Caffeic acid, benzyl caffeate, phenethyl caffeate, 5-methoxy pinobanksin, pinobanksin, pinocembrin, pinobanksin-3-O-acetate, chrysin, and galangin were the characteristic compounds of Beijing propolis, and these compounds seem to verify that Beijing propolis may be poplar-type propolis. In addition, the presence of high level of pinobanksin-3-O-acetate in Chinese propolis may be a novel finding, representing one-third of all phenolics.

No MeSH data available.


Related in: MedlinePlus

HPLC chromatograms of different EEP (recorded at 280nm). (1) 3,4-Dihydroxybenzaldehyde, (2) vanillic acid, (3) caffeic acid, (4) p-coumaric acid, (5) ferulic acid, (6) isoferulic acid, (7) benzoic acid, (8) 3,4-dimethoxycinnamic acid, (9) cinnamic acid, (10) 4-methoxycinnamic acid, (11) cinnamylideneacetic acid, (12) 5-methoxy pinobanksin, (13) pinobanksin, (14) quercetin, (15) alpinetin, (16) kaempferol, (17) cinnamylideneacetic acid, (18) apigenin, (19) isorhamnetin, (20) pinocembrin, (21) benzyl caffeate, (22) pinobanksin-3-O-acetate, (23) chrysin, (24) phenethyl caffeate, (25) galangin, (26) benzyl p-coumarate, (27) pinostrobin, (28) tectochrysin, and (29) cinnamyl cinnamate.
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fig1: HPLC chromatograms of different EEP (recorded at 280nm). (1) 3,4-Dihydroxybenzaldehyde, (2) vanillic acid, (3) caffeic acid, (4) p-coumaric acid, (5) ferulic acid, (6) isoferulic acid, (7) benzoic acid, (8) 3,4-dimethoxycinnamic acid, (9) cinnamic acid, (10) 4-methoxycinnamic acid, (11) cinnamylideneacetic acid, (12) 5-methoxy pinobanksin, (13) pinobanksin, (14) quercetin, (15) alpinetin, (16) kaempferol, (17) cinnamylideneacetic acid, (18) apigenin, (19) isorhamnetin, (20) pinocembrin, (21) benzyl caffeate, (22) pinobanksin-3-O-acetate, (23) chrysin, (24) phenethyl caffeate, (25) galangin, (26) benzyl p-coumarate, (27) pinostrobin, (28) tectochrysin, and (29) cinnamyl cinnamate.

Mentions: Phenolic composition of propolis extracts was identified by HPLC-PDA-MS. Figure 1 shows the HPLC profiles of propolis extracts by using different ethanol/water solvents. According to the HPLC-PDA profiles of propolis extracts and UV spectra of major peaks, these extracts can be classified into two types. The first type, including WEP and 25% EEP, exhibited simple chromatograms with limited amount of peaks which mainly located in the region of retention times up to 50 min. The second type, including 50, 75, 95, and 100% EEP, presented the complicated chromatograms. HPLC profiles of these extracts were extremely complex with many peaks between the retention times of 10 and 140 min.


Effect of Ethanol/Water Solvents on Phenolic Profiles and Antioxidant Properties of Beijing Propolis Extracts.

Sun C, Wu Z, Wang Z, Zhang H - Evid Based Complement Alternat Med (2015)

HPLC chromatograms of different EEP (recorded at 280nm). (1) 3,4-Dihydroxybenzaldehyde, (2) vanillic acid, (3) caffeic acid, (4) p-coumaric acid, (5) ferulic acid, (6) isoferulic acid, (7) benzoic acid, (8) 3,4-dimethoxycinnamic acid, (9) cinnamic acid, (10) 4-methoxycinnamic acid, (11) cinnamylideneacetic acid, (12) 5-methoxy pinobanksin, (13) pinobanksin, (14) quercetin, (15) alpinetin, (16) kaempferol, (17) cinnamylideneacetic acid, (18) apigenin, (19) isorhamnetin, (20) pinocembrin, (21) benzyl caffeate, (22) pinobanksin-3-O-acetate, (23) chrysin, (24) phenethyl caffeate, (25) galangin, (26) benzyl p-coumarate, (27) pinostrobin, (28) tectochrysin, and (29) cinnamyl cinnamate.
© Copyright Policy - open-access
Related In: Results  -  Collection

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getmorefigures.php?uid=PMC4553316&req=5

fig1: HPLC chromatograms of different EEP (recorded at 280nm). (1) 3,4-Dihydroxybenzaldehyde, (2) vanillic acid, (3) caffeic acid, (4) p-coumaric acid, (5) ferulic acid, (6) isoferulic acid, (7) benzoic acid, (8) 3,4-dimethoxycinnamic acid, (9) cinnamic acid, (10) 4-methoxycinnamic acid, (11) cinnamylideneacetic acid, (12) 5-methoxy pinobanksin, (13) pinobanksin, (14) quercetin, (15) alpinetin, (16) kaempferol, (17) cinnamylideneacetic acid, (18) apigenin, (19) isorhamnetin, (20) pinocembrin, (21) benzyl caffeate, (22) pinobanksin-3-O-acetate, (23) chrysin, (24) phenethyl caffeate, (25) galangin, (26) benzyl p-coumarate, (27) pinostrobin, (28) tectochrysin, and (29) cinnamyl cinnamate.
Mentions: Phenolic composition of propolis extracts was identified by HPLC-PDA-MS. Figure 1 shows the HPLC profiles of propolis extracts by using different ethanol/water solvents. According to the HPLC-PDA profiles of propolis extracts and UV spectra of major peaks, these extracts can be classified into two types. The first type, including WEP and 25% EEP, exhibited simple chromatograms with limited amount of peaks which mainly located in the region of retention times up to 50 min. The second type, including 50, 75, 95, and 100% EEP, presented the complicated chromatograms. HPLC profiles of these extracts were extremely complex with many peaks between the retention times of 10 and 140 min.

Bottom Line: Our results reveal that phenolic compounds and antioxidant properties of propolis extracts were significantly dependent on the concentration of ethanol/water solvents.In particular, 75 wt.% ethanol/water solvent may be the best for the highest extraction yield and the strongest antioxidant properties.Caffeic acid, benzyl caffeate, phenethyl caffeate, 5-methoxy pinobanksin, pinobanksin, pinocembrin, pinobanksin-3-O-acetate, chrysin, and galangin were the characteristic compounds of Beijing propolis, and these compounds seem to verify that Beijing propolis may be poplar-type propolis.

View Article: PubMed Central - PubMed

Affiliation: Institute of Apicultural Research, Chinese Academy of Agricultural Sciences, Beijing 100093, China.

ABSTRACT
Propolis is a natural substance known to be beneficial for human health and used as a folk medicine in many parts of the world. In this study, phenolic profiles and antioxidant properties of Beijing propolis extracted by different ethanol/water solvents were analyzed. Our results reveal that phenolic compounds and antioxidant properties of propolis extracts were significantly dependent on the concentration of ethanol/water solvents. Totally, 29 phenolic compounds were identified: 12 phenolic acids, 13 flavonoids, and 4 phenolic acid esters. In particular, 75 wt.% ethanol/water solvent may be the best for the highest extraction yield and the strongest antioxidant properties. Caffeic acid, benzyl caffeate, phenethyl caffeate, 5-methoxy pinobanksin, pinobanksin, pinocembrin, pinobanksin-3-O-acetate, chrysin, and galangin were the characteristic compounds of Beijing propolis, and these compounds seem to verify that Beijing propolis may be poplar-type propolis. In addition, the presence of high level of pinobanksin-3-O-acetate in Chinese propolis may be a novel finding, representing one-third of all phenolics.

No MeSH data available.


Related in: MedlinePlus