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Studies on the Antifatigue Activities of Cordyceps militaris Fruit Body Extract in Mouse Model.

Song J, Wang Y, Teng M, Cai G, Xu H, Guo H, Liu Y, Wang D, Teng L - Evid Based Complement Alternat Med (2015)

Bottom Line: Cordyceps militaris has been used extensively as a crude drug and a folk tonic food in East Asia due to its various pharmacological activities.Moreover, CM-enhanced the phosphorylation of AMPK contributes to its antioxidant effect.Our data provides experimental evidence in supporting clinical use of CM as an effective agent against fatigue.

View Article: PubMed Central - PubMed

Affiliation: College of Life Sciences, Jilin University, Changchun 130012, China.

ABSTRACT
Cordyceps militaris has been used extensively as a crude drug and a folk tonic food in East Asia due to its various pharmacological activities. Our study aims to investigate the effect of Cordyceps militaris fruit body extract (CM) on antifatigue in mouse model. Two week CM administration significantly delayed fatigue phenomenon which is confirmed via rotating rod test, forced swimming test and forced running test. Compared to nontreated mouse, CM administration increased ATP levels and antioxidative enzymes activity and reduced the levels of lactic acid, lactic dehydrogenase, malondialdehyde, and reactive oxygen species. Further data suggests that CM-induced fatigue recovery is mainly through activating 5'-AMP-activated protein kinase (AMPK) and protein kinase B (AKT)/mammalian target of rapamycin (mTOR) pathways and regulating serum hormone level. Moreover, CM-enhanced the phosphorylation of AMPK contributes to its antioxidant effect. Our data provides experimental evidence in supporting clinical use of CM as an effective agent against fatigue.

No MeSH data available.


Related in: MedlinePlus

Mice were treated with Cordyceps militaris fruit body extract (0.5 g/kg, 1.0 g/kg, and 2.0 g/kg) and Rhodiola rosea (0.5 g/kg) for 14 days. Before and after 20 min swimming, the serum levels of estradiol ((a), (b)), testosterone ((c), (d)), and cortisol ((e), (f)) in both male and female mouse were determined via ELISA method. Data are expressed as mean ± S.D. (n = 6) and analyzed using one-way ANOVA followed by Dunn's test. ∗P < 0.05 and ∗∗P < 0.01 versus control group.
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fig6: Mice were treated with Cordyceps militaris fruit body extract (0.5 g/kg, 1.0 g/kg, and 2.0 g/kg) and Rhodiola rosea (0.5 g/kg) for 14 days. Before and after 20 min swimming, the serum levels of estradiol ((a), (b)), testosterone ((c), (d)), and cortisol ((e), (f)) in both male and female mouse were determined via ELISA method. Data are expressed as mean ± S.D. (n = 6) and analyzed using one-way ANOVA followed by Dunn's test. ∗P < 0.05 and ∗∗P < 0.01 versus control group.

Mentions: Since CM displayed different efficacy in male and female mouse, serum hormones including E2, T, and cortisol were detected after 14-day CM treatment. Both CM and Rhodiola rosea treatment enhanced serum E2 concentration in female mouse before and after 20 min swimming (P < 0.05; Figure 6(a)). Interestingly, compared to nontreated group, only CM at a dose of 2.0 g/kg increase 15.1% serum E2 in male mouse after swimming (P < 0.05; Figure 6(b)). In male and female mouse, the increased serum T levels were observed in CM and Rhodiola rosea-treated group before and after exercise (P < 0.05; Figures 6(c) and 6(d)). For serum cortisol level, treatment with 1.0 g/kg and 2.0 g/kg CM resulted in 33.5% and 18.6% increment after swimming in female mouse (P < 0.05; Figure 6(e)); however, in male mouse, 2.0 g/kg CM treatment increased nearly 34.6% and 24.5% before and after exercise (P < 0.05; Figure 6(f)).


Studies on the Antifatigue Activities of Cordyceps militaris Fruit Body Extract in Mouse Model.

Song J, Wang Y, Teng M, Cai G, Xu H, Guo H, Liu Y, Wang D, Teng L - Evid Based Complement Alternat Med (2015)

Mice were treated with Cordyceps militaris fruit body extract (0.5 g/kg, 1.0 g/kg, and 2.0 g/kg) and Rhodiola rosea (0.5 g/kg) for 14 days. Before and after 20 min swimming, the serum levels of estradiol ((a), (b)), testosterone ((c), (d)), and cortisol ((e), (f)) in both male and female mouse were determined via ELISA method. Data are expressed as mean ± S.D. (n = 6) and analyzed using one-way ANOVA followed by Dunn's test. ∗P < 0.05 and ∗∗P < 0.01 versus control group.
© Copyright Policy - open-access
Related In: Results  -  Collection

Show All Figures
getmorefigures.php?uid=PMC4553310&req=5

fig6: Mice were treated with Cordyceps militaris fruit body extract (0.5 g/kg, 1.0 g/kg, and 2.0 g/kg) and Rhodiola rosea (0.5 g/kg) for 14 days. Before and after 20 min swimming, the serum levels of estradiol ((a), (b)), testosterone ((c), (d)), and cortisol ((e), (f)) in both male and female mouse were determined via ELISA method. Data are expressed as mean ± S.D. (n = 6) and analyzed using one-way ANOVA followed by Dunn's test. ∗P < 0.05 and ∗∗P < 0.01 versus control group.
Mentions: Since CM displayed different efficacy in male and female mouse, serum hormones including E2, T, and cortisol were detected after 14-day CM treatment. Both CM and Rhodiola rosea treatment enhanced serum E2 concentration in female mouse before and after 20 min swimming (P < 0.05; Figure 6(a)). Interestingly, compared to nontreated group, only CM at a dose of 2.0 g/kg increase 15.1% serum E2 in male mouse after swimming (P < 0.05; Figure 6(b)). In male and female mouse, the increased serum T levels were observed in CM and Rhodiola rosea-treated group before and after exercise (P < 0.05; Figures 6(c) and 6(d)). For serum cortisol level, treatment with 1.0 g/kg and 2.0 g/kg CM resulted in 33.5% and 18.6% increment after swimming in female mouse (P < 0.05; Figure 6(e)); however, in male mouse, 2.0 g/kg CM treatment increased nearly 34.6% and 24.5% before and after exercise (P < 0.05; Figure 6(f)).

Bottom Line: Cordyceps militaris has been used extensively as a crude drug and a folk tonic food in East Asia due to its various pharmacological activities.Moreover, CM-enhanced the phosphorylation of AMPK contributes to its antioxidant effect.Our data provides experimental evidence in supporting clinical use of CM as an effective agent against fatigue.

View Article: PubMed Central - PubMed

Affiliation: College of Life Sciences, Jilin University, Changchun 130012, China.

ABSTRACT
Cordyceps militaris has been used extensively as a crude drug and a folk tonic food in East Asia due to its various pharmacological activities. Our study aims to investigate the effect of Cordyceps militaris fruit body extract (CM) on antifatigue in mouse model. Two week CM administration significantly delayed fatigue phenomenon which is confirmed via rotating rod test, forced swimming test and forced running test. Compared to nontreated mouse, CM administration increased ATP levels and antioxidative enzymes activity and reduced the levels of lactic acid, lactic dehydrogenase, malondialdehyde, and reactive oxygen species. Further data suggests that CM-induced fatigue recovery is mainly through activating 5'-AMP-activated protein kinase (AMPK) and protein kinase B (AKT)/mammalian target of rapamycin (mTOR) pathways and regulating serum hormone level. Moreover, CM-enhanced the phosphorylation of AMPK contributes to its antioxidant effect. Our data provides experimental evidence in supporting clinical use of CM as an effective agent against fatigue.

No MeSH data available.


Related in: MedlinePlus