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Studies on the Antifatigue Activities of Cordyceps militaris Fruit Body Extract in Mouse Model.

Song J, Wang Y, Teng M, Cai G, Xu H, Guo H, Liu Y, Wang D, Teng L - Evid Based Complement Alternat Med (2015)

Bottom Line: Cordyceps militaris has been used extensively as a crude drug and a folk tonic food in East Asia due to its various pharmacological activities.Moreover, CM-enhanced the phosphorylation of AMPK contributes to its antioxidant effect.Our data provides experimental evidence in supporting clinical use of CM as an effective agent against fatigue.

View Article: PubMed Central - PubMed

Affiliation: College of Life Sciences, Jilin University, Changchun 130012, China.

ABSTRACT
Cordyceps militaris has been used extensively as a crude drug and a folk tonic food in East Asia due to its various pharmacological activities. Our study aims to investigate the effect of Cordyceps militaris fruit body extract (CM) on antifatigue in mouse model. Two week CM administration significantly delayed fatigue phenomenon which is confirmed via rotating rod test, forced swimming test and forced running test. Compared to nontreated mouse, CM administration increased ATP levels and antioxidative enzymes activity and reduced the levels of lactic acid, lactic dehydrogenase, malondialdehyde, and reactive oxygen species. Further data suggests that CM-induced fatigue recovery is mainly through activating 5'-AMP-activated protein kinase (AMPK) and protein kinase B (AKT)/mammalian target of rapamycin (mTOR) pathways and regulating serum hormone level. Moreover, CM-enhanced the phosphorylation of AMPK contributes to its antioxidant effect. Our data provides experimental evidence in supporting clinical use of CM as an effective agent against fatigue.

No MeSH data available.


Related in: MedlinePlus

Mice were treated with Cordyceps militaris fruit body extract (0.5 g/kg, 1.0 g/kg, and 2.0 g/kg) and Rhodiola rosea (0.5 g/kg) for 14 days. After 20 min swimming, the activations of ERKs, AKT, mTOR, and AMPK in liver tissue were detected via western blot. Quantification data of the expression of P-ERKs, P-AKT, P-mTOR, and P-AMPK were normalized by corresponding T-ERKs, T-AKT, T-mTOR, and T-AMPK. Data are expressed as mean ± S.D. (n = 6) and analyzed using one-way ANOVA followed by Dunn's test. ∗P < 0.05 and ∗∗P < 0.01 versus control group.
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Related In: Results  -  Collection


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fig5: Mice were treated with Cordyceps militaris fruit body extract (0.5 g/kg, 1.0 g/kg, and 2.0 g/kg) and Rhodiola rosea (0.5 g/kg) for 14 days. After 20 min swimming, the activations of ERKs, AKT, mTOR, and AMPK in liver tissue were detected via western blot. Quantification data of the expression of P-ERKs, P-AKT, P-mTOR, and P-AMPK were normalized by corresponding T-ERKs, T-AKT, T-mTOR, and T-AMPK. Data are expressed as mean ± S.D. (n = 6) and analyzed using one-way ANOVA followed by Dunn's test. ∗P < 0.05 and ∗∗P < 0.01 versus control group.

Mentions: In order to investigate the preliminary mechanisms during CM-mediated antifatigue activity, the phosphorylation of ERKs, AKT, mTOR, and AMPK in liver tissue was detected via western blot. Similar to the enhanced activity of Rhodiola rosea on protein levels, 14-day CM administration resulted in 96.2%, 79.6%, 32.9%, and 160.5% increment on the expressions of P-ERKs, P-AKT, P-mTOR, and P-AMPK (P < 0.05; Figure 5).


Studies on the Antifatigue Activities of Cordyceps militaris Fruit Body Extract in Mouse Model.

Song J, Wang Y, Teng M, Cai G, Xu H, Guo H, Liu Y, Wang D, Teng L - Evid Based Complement Alternat Med (2015)

Mice were treated with Cordyceps militaris fruit body extract (0.5 g/kg, 1.0 g/kg, and 2.0 g/kg) and Rhodiola rosea (0.5 g/kg) for 14 days. After 20 min swimming, the activations of ERKs, AKT, mTOR, and AMPK in liver tissue were detected via western blot. Quantification data of the expression of P-ERKs, P-AKT, P-mTOR, and P-AMPK were normalized by corresponding T-ERKs, T-AKT, T-mTOR, and T-AMPK. Data are expressed as mean ± S.D. (n = 6) and analyzed using one-way ANOVA followed by Dunn's test. ∗P < 0.05 and ∗∗P < 0.01 versus control group.
© Copyright Policy - open-access
Related In: Results  -  Collection

Show All Figures
getmorefigures.php?uid=PMC4553310&req=5

fig5: Mice were treated with Cordyceps militaris fruit body extract (0.5 g/kg, 1.0 g/kg, and 2.0 g/kg) and Rhodiola rosea (0.5 g/kg) for 14 days. After 20 min swimming, the activations of ERKs, AKT, mTOR, and AMPK in liver tissue were detected via western blot. Quantification data of the expression of P-ERKs, P-AKT, P-mTOR, and P-AMPK were normalized by corresponding T-ERKs, T-AKT, T-mTOR, and T-AMPK. Data are expressed as mean ± S.D. (n = 6) and analyzed using one-way ANOVA followed by Dunn's test. ∗P < 0.05 and ∗∗P < 0.01 versus control group.
Mentions: In order to investigate the preliminary mechanisms during CM-mediated antifatigue activity, the phosphorylation of ERKs, AKT, mTOR, and AMPK in liver tissue was detected via western blot. Similar to the enhanced activity of Rhodiola rosea on protein levels, 14-day CM administration resulted in 96.2%, 79.6%, 32.9%, and 160.5% increment on the expressions of P-ERKs, P-AKT, P-mTOR, and P-AMPK (P < 0.05; Figure 5).

Bottom Line: Cordyceps militaris has been used extensively as a crude drug and a folk tonic food in East Asia due to its various pharmacological activities.Moreover, CM-enhanced the phosphorylation of AMPK contributes to its antioxidant effect.Our data provides experimental evidence in supporting clinical use of CM as an effective agent against fatigue.

View Article: PubMed Central - PubMed

Affiliation: College of Life Sciences, Jilin University, Changchun 130012, China.

ABSTRACT
Cordyceps militaris has been used extensively as a crude drug and a folk tonic food in East Asia due to its various pharmacological activities. Our study aims to investigate the effect of Cordyceps militaris fruit body extract (CM) on antifatigue in mouse model. Two week CM administration significantly delayed fatigue phenomenon which is confirmed via rotating rod test, forced swimming test and forced running test. Compared to nontreated mouse, CM administration increased ATP levels and antioxidative enzymes activity and reduced the levels of lactic acid, lactic dehydrogenase, malondialdehyde, and reactive oxygen species. Further data suggests that CM-induced fatigue recovery is mainly through activating 5'-AMP-activated protein kinase (AMPK) and protein kinase B (AKT)/mammalian target of rapamycin (mTOR) pathways and regulating serum hormone level. Moreover, CM-enhanced the phosphorylation of AMPK contributes to its antioxidant effect. Our data provides experimental evidence in supporting clinical use of CM as an effective agent against fatigue.

No MeSH data available.


Related in: MedlinePlus