Limits...
Studies on the Antifatigue Activities of Cordyceps militaris Fruit Body Extract in Mouse Model.

Song J, Wang Y, Teng M, Cai G, Xu H, Guo H, Liu Y, Wang D, Teng L - Evid Based Complement Alternat Med (2015)

Bottom Line: Cordyceps militaris has been used extensively as a crude drug and a folk tonic food in East Asia due to its various pharmacological activities.Moreover, CM-enhanced the phosphorylation of AMPK contributes to its antioxidant effect.Our data provides experimental evidence in supporting clinical use of CM as an effective agent against fatigue.

View Article: PubMed Central - PubMed

Affiliation: College of Life Sciences, Jilin University, Changchun 130012, China.

ABSTRACT
Cordyceps militaris has been used extensively as a crude drug and a folk tonic food in East Asia due to its various pharmacological activities. Our study aims to investigate the effect of Cordyceps militaris fruit body extract (CM) on antifatigue in mouse model. Two week CM administration significantly delayed fatigue phenomenon which is confirmed via rotating rod test, forced swimming test and forced running test. Compared to nontreated mouse, CM administration increased ATP levels and antioxidative enzymes activity and reduced the levels of lactic acid, lactic dehydrogenase, malondialdehyde, and reactive oxygen species. Further data suggests that CM-induced fatigue recovery is mainly through activating 5'-AMP-activated protein kinase (AMPK) and protein kinase B (AKT)/mammalian target of rapamycin (mTOR) pathways and regulating serum hormone level. Moreover, CM-enhanced the phosphorylation of AMPK contributes to its antioxidant effect. Our data provides experimental evidence in supporting clinical use of CM as an effective agent against fatigue.

No MeSH data available.


Related in: MedlinePlus

Treatment with Cordyceps militaris fruit body extract (0.5 g/kg, 1.0 g/kg, and 2.0 g/kg) and Rhodiola rosea (0.5 g/kg) for 2 weeks, before and after 20 min swimming, the levels of lactate dehydrogenase in serum ((a), (b)), liver ((c), (d)), and muscle ((e), (f)) in male and female mouse were analyzed, respectively. Data are expressed as mean ± S.D. (n = 6) and analyzed using one-way ANOVA followed by Dunn's test. ∗P < 0.05 and ∗∗P < 0.01 versus nontreated mouse (Control).
© Copyright Policy - open-access
Related In: Results  -  Collection


getmorefigures.php?uid=PMC4553310&req=5

fig3: Treatment with Cordyceps militaris fruit body extract (0.5 g/kg, 1.0 g/kg, and 2.0 g/kg) and Rhodiola rosea (0.5 g/kg) for 2 weeks, before and after 20 min swimming, the levels of lactate dehydrogenase in serum ((a), (b)), liver ((c), (d)), and muscle ((e), (f)) in male and female mouse were analyzed, respectively. Data are expressed as mean ± S.D. (n = 6) and analyzed using one-way ANOVA followed by Dunn's test. ∗P < 0.05 and ∗∗P < 0.01 versus nontreated mouse (Control).

Mentions: LDH, an accurate indicator of muscle damage, catalyzes the interconversion of pyruvate and lactate [22], which is determined in serum, liver, and muscle of all experimental mice. Before excise, compared to control group, 14-day CM treatment reduced 20.3% (at a dose of 2.0 mg/kg) and 17.4% (at a dose of 1.0 mg/kg) serum LDH activity in female mouse and male mouse, respectively (P < 0.05; Figures 3(a) and 3(d)). Similarly, 0.5 g/kg Rhodiola rosea only influenced serum LDH activity before swimming. After 20 min exercise, in CM-treated male and female mouse, LDH activity was suppressed in serum, liver, and muscle in different degree (P < 0.05; Figure 3).


Studies on the Antifatigue Activities of Cordyceps militaris Fruit Body Extract in Mouse Model.

Song J, Wang Y, Teng M, Cai G, Xu H, Guo H, Liu Y, Wang D, Teng L - Evid Based Complement Alternat Med (2015)

Treatment with Cordyceps militaris fruit body extract (0.5 g/kg, 1.0 g/kg, and 2.0 g/kg) and Rhodiola rosea (0.5 g/kg) for 2 weeks, before and after 20 min swimming, the levels of lactate dehydrogenase in serum ((a), (b)), liver ((c), (d)), and muscle ((e), (f)) in male and female mouse were analyzed, respectively. Data are expressed as mean ± S.D. (n = 6) and analyzed using one-way ANOVA followed by Dunn's test. ∗P < 0.05 and ∗∗P < 0.01 versus nontreated mouse (Control).
© Copyright Policy - open-access
Related In: Results  -  Collection

Show All Figures
getmorefigures.php?uid=PMC4553310&req=5

fig3: Treatment with Cordyceps militaris fruit body extract (0.5 g/kg, 1.0 g/kg, and 2.0 g/kg) and Rhodiola rosea (0.5 g/kg) for 2 weeks, before and after 20 min swimming, the levels of lactate dehydrogenase in serum ((a), (b)), liver ((c), (d)), and muscle ((e), (f)) in male and female mouse were analyzed, respectively. Data are expressed as mean ± S.D. (n = 6) and analyzed using one-way ANOVA followed by Dunn's test. ∗P < 0.05 and ∗∗P < 0.01 versus nontreated mouse (Control).
Mentions: LDH, an accurate indicator of muscle damage, catalyzes the interconversion of pyruvate and lactate [22], which is determined in serum, liver, and muscle of all experimental mice. Before excise, compared to control group, 14-day CM treatment reduced 20.3% (at a dose of 2.0 mg/kg) and 17.4% (at a dose of 1.0 mg/kg) serum LDH activity in female mouse and male mouse, respectively (P < 0.05; Figures 3(a) and 3(d)). Similarly, 0.5 g/kg Rhodiola rosea only influenced serum LDH activity before swimming. After 20 min exercise, in CM-treated male and female mouse, LDH activity was suppressed in serum, liver, and muscle in different degree (P < 0.05; Figure 3).

Bottom Line: Cordyceps militaris has been used extensively as a crude drug and a folk tonic food in East Asia due to its various pharmacological activities.Moreover, CM-enhanced the phosphorylation of AMPK contributes to its antioxidant effect.Our data provides experimental evidence in supporting clinical use of CM as an effective agent against fatigue.

View Article: PubMed Central - PubMed

Affiliation: College of Life Sciences, Jilin University, Changchun 130012, China.

ABSTRACT
Cordyceps militaris has been used extensively as a crude drug and a folk tonic food in East Asia due to its various pharmacological activities. Our study aims to investigate the effect of Cordyceps militaris fruit body extract (CM) on antifatigue in mouse model. Two week CM administration significantly delayed fatigue phenomenon which is confirmed via rotating rod test, forced swimming test and forced running test. Compared to nontreated mouse, CM administration increased ATP levels and antioxidative enzymes activity and reduced the levels of lactic acid, lactic dehydrogenase, malondialdehyde, and reactive oxygen species. Further data suggests that CM-induced fatigue recovery is mainly through activating 5'-AMP-activated protein kinase (AMPK) and protein kinase B (AKT)/mammalian target of rapamycin (mTOR) pathways and regulating serum hormone level. Moreover, CM-enhanced the phosphorylation of AMPK contributes to its antioxidant effect. Our data provides experimental evidence in supporting clinical use of CM as an effective agent against fatigue.

No MeSH data available.


Related in: MedlinePlus