Macrophage-Induced Blood Vessels Guide Schwann Cell-Mediated Regeneration of Peripheral Nerves.
Bottom Line: Here we show that blood vessels direct the migrating cords of Schwann cells.Importantly, disrupting the organization of the newly formed blood vessels in vivo, either by inhibiting the angiogenic signal or by re-orienting them, compromises Schwann cell directionality resulting in defective nerve repair.This study provides important insights into how the choreography of multiple cell-types is required for the regeneration of an adult tissue.
Affiliation: MRC Laboratory for Molecular Cell Biology, UCL, Gower Street, London WC1E 6BT, UK.Show MeSH
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Mentions: Analysis of the incorporation of EdU found that all the blood vessels within the bridge contained EdU-positive ECs, confirming that they were newly formed (Figures 2A, 2B, and S2A). In contrast, EdU-positive ECs were not found within uncut nerve (Figures 2A and 2B) or in the proximal or distal stumps (Figure S2B). Moreover, erythrocyte staining and the detection of injected immunolabeled-lectin showed that the majority of the newly formed blood vessels within the bridge were functional (Figures S2C and S2D).
Affiliation: MRC Laboratory for Molecular Cell Biology, UCL, Gower Street, London WC1E 6BT, UK.