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Cytokine serum levels during post-transplant adverse events in 61 pediatric patients after hematopoietic stem cell transplantation.

Döring M, Cabanillas Stanchi KM, Mezger M, Erbacher A, Feucht J, Pfeiffer M, Lang P, Handgretinger R, Müller I - BMC Cancer (2015)

Bottom Line: Veno-occlusive disease was accompanied by a significant increase in levels of IL-6, IL-8 and TNF-α.Graft-versus-Host disease was associated with a significant increase of IL-10, sIL-2R, IL-6 and TNF-α, depending on the respective stage or grade.Cytokine IL-6 enabled a significant differentiation between sepsis and fungemia, sepsis and viremia, and sepsis and bacteremia.Moreover, cytokine IL-8 enabled a significant differentiation between sepsis and viremia, sepsis and bacteremia, and bacteremia and viremia whereas IL-10 made a distinction between sepsis and viremia possible.

View Article: PubMed Central - PubMed

Affiliation: Department I - General Paediatrics, Hematology/Oncology, University Hospital Tuebingen, Children's Hospital, Hoppe-Seyler-Str. 1, 72076, Tuebingen, Germany. michaela.doering@med.uni-tuebingen.de.

ABSTRACT

Background: Veno-occlusive disease, Graft-versus-Host disease, invasive or localized bacterial, viral and fungal infections are known as adverse events after hematopoietic stem cell transplantation representing the major cause for morbidity and mortality. Detection and differentiation of these adverse events are based on clinical symptoms and routine measurements of laboratory parameters.

Methods: To identify the role of cytokines as a possible complication-marker for adverse events, 61 consecutive pediatric patients with a median age of 7.0 years who underwent hematopoietic stem cell transplantation were enrolled in this single-center retrospective study. Interleukin-1 beta (IL-1β), soluble interleukin-2 receptor (sIL-2R), interleukin-6 (IL-6), interleukin-8 (IL-8), interleukin-10 (IL-10) and tumor necrosis factor-α serum (TNF-α) levels were regularly assessed after transplantation and during transplantation related adverse events.

Results: Veno-occlusive disease was accompanied by a significant increase in levels of IL-6, IL-8 and TNF-α.Graft-versus-Host disease was associated with a significant increase of IL-10, sIL-2R, IL-6 and TNF-α, depending on the respective stage or grade. Cytokine IL-6 enabled a significant differentiation between sepsis and fungemia, sepsis and viremia, and sepsis and bacteremia. Moreover, cytokine IL-8 enabled a significant differentiation between sepsis and viremia, sepsis and bacteremia, and bacteremia and viremia whereas IL-10 made a distinction between sepsis and viremia possible.

Conclusion: The data demonstrate that proinflammatory cytokines might be putative indicators for early detection and differentiation of post-transplant adverse events and may allow prompt and adequate clinical intervention. Prospective clinical trials are needed to evaluate these findings.

No MeSH data available.


Related in: MedlinePlus

TNF-α concentrations during post-transplant infectious complications. Data show mean TNF-α serum concentration in occurrence of sepsis (55.42 ± 63.04 pg/ml), bacteremia (25.95 ± 68.32 pg/ml), viremia (18.88 ± 22.64 pg/ml) and fungemia (13.37 ± 12.60 pg/ml). Data show mean +95 % CI
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Fig6: TNF-α concentrations during post-transplant infectious complications. Data show mean TNF-α serum concentration in occurrence of sepsis (55.42 ± 63.04 pg/ml), bacteremia (25.95 ± 68.32 pg/ml), viremia (18.88 ± 22.64 pg/ml) and fungemia (13.37 ± 12.60 pg/ml). Data show mean +95 % CI

Mentions: After Bonferroni correction, there was no significant decrease (P = 0.046) of TNF-α between sepsis (55.42 ± 63.04 pg/ml) and fungemia (13.37 ± 12.60 pg/ml). Sepsis compared to bacteremia (25.95 ± 68.32; P = 0.85) and to viremia (18.88 ± 22.64; P = 0.322) showed no significant changes in TNF-α serum concentrations. Moreover, the comparison of TNF-α concentrations in occurrence of bacteremia in relation to both fungemia (P = 0.614) and viremia (P = 0.884) was not significant. A comparison of the TNF-α concentration between viremia and fungemia also showed no significance (P = 0.091) after Bonferroni correction (adjusted α = 0.0083) (Fig. 6).Fig. 6


Cytokine serum levels during post-transplant adverse events in 61 pediatric patients after hematopoietic stem cell transplantation.

Döring M, Cabanillas Stanchi KM, Mezger M, Erbacher A, Feucht J, Pfeiffer M, Lang P, Handgretinger R, Müller I - BMC Cancer (2015)

TNF-α concentrations during post-transplant infectious complications. Data show mean TNF-α serum concentration in occurrence of sepsis (55.42 ± 63.04 pg/ml), bacteremia (25.95 ± 68.32 pg/ml), viremia (18.88 ± 22.64 pg/ml) and fungemia (13.37 ± 12.60 pg/ml). Data show mean +95 % CI
© Copyright Policy - OpenAccess
Related In: Results  -  Collection

License 1 - License 2
Show All Figures
getmorefigures.php?uid=PMC4552308&req=5

Fig6: TNF-α concentrations during post-transplant infectious complications. Data show mean TNF-α serum concentration in occurrence of sepsis (55.42 ± 63.04 pg/ml), bacteremia (25.95 ± 68.32 pg/ml), viremia (18.88 ± 22.64 pg/ml) and fungemia (13.37 ± 12.60 pg/ml). Data show mean +95 % CI
Mentions: After Bonferroni correction, there was no significant decrease (P = 0.046) of TNF-α between sepsis (55.42 ± 63.04 pg/ml) and fungemia (13.37 ± 12.60 pg/ml). Sepsis compared to bacteremia (25.95 ± 68.32; P = 0.85) and to viremia (18.88 ± 22.64; P = 0.322) showed no significant changes in TNF-α serum concentrations. Moreover, the comparison of TNF-α concentrations in occurrence of bacteremia in relation to both fungemia (P = 0.614) and viremia (P = 0.884) was not significant. A comparison of the TNF-α concentration between viremia and fungemia also showed no significance (P = 0.091) after Bonferroni correction (adjusted α = 0.0083) (Fig. 6).Fig. 6

Bottom Line: Veno-occlusive disease was accompanied by a significant increase in levels of IL-6, IL-8 and TNF-α.Graft-versus-Host disease was associated with a significant increase of IL-10, sIL-2R, IL-6 and TNF-α, depending on the respective stage or grade.Cytokine IL-6 enabled a significant differentiation between sepsis and fungemia, sepsis and viremia, and sepsis and bacteremia.Moreover, cytokine IL-8 enabled a significant differentiation between sepsis and viremia, sepsis and bacteremia, and bacteremia and viremia whereas IL-10 made a distinction between sepsis and viremia possible.

View Article: PubMed Central - PubMed

Affiliation: Department I - General Paediatrics, Hematology/Oncology, University Hospital Tuebingen, Children's Hospital, Hoppe-Seyler-Str. 1, 72076, Tuebingen, Germany. michaela.doering@med.uni-tuebingen.de.

ABSTRACT

Background: Veno-occlusive disease, Graft-versus-Host disease, invasive or localized bacterial, viral and fungal infections are known as adverse events after hematopoietic stem cell transplantation representing the major cause for morbidity and mortality. Detection and differentiation of these adverse events are based on clinical symptoms and routine measurements of laboratory parameters.

Methods: To identify the role of cytokines as a possible complication-marker for adverse events, 61 consecutive pediatric patients with a median age of 7.0 years who underwent hematopoietic stem cell transplantation were enrolled in this single-center retrospective study. Interleukin-1 beta (IL-1β), soluble interleukin-2 receptor (sIL-2R), interleukin-6 (IL-6), interleukin-8 (IL-8), interleukin-10 (IL-10) and tumor necrosis factor-α serum (TNF-α) levels were regularly assessed after transplantation and during transplantation related adverse events.

Results: Veno-occlusive disease was accompanied by a significant increase in levels of IL-6, IL-8 and TNF-α.Graft-versus-Host disease was associated with a significant increase of IL-10, sIL-2R, IL-6 and TNF-α, depending on the respective stage or grade. Cytokine IL-6 enabled a significant differentiation between sepsis and fungemia, sepsis and viremia, and sepsis and bacteremia. Moreover, cytokine IL-8 enabled a significant differentiation between sepsis and viremia, sepsis and bacteremia, and bacteremia and viremia whereas IL-10 made a distinction between sepsis and viremia possible.

Conclusion: The data demonstrate that proinflammatory cytokines might be putative indicators for early detection and differentiation of post-transplant adverse events and may allow prompt and adequate clinical intervention. Prospective clinical trials are needed to evaluate these findings.

No MeSH data available.


Related in: MedlinePlus