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Effect of porous polycaprolactone beads on bone regeneration: preliminary in vitro and in vivo studies.

Byun JH, Lee HA, Kim TH, Lee JH, Oh SH - Biomater Res (2014)

Bottom Line: For the effective bone regeneration with appropriate pathological/physiological properties, a variety of bone fillers have been adapted as a therapeutic treatment.For the preliminary animal study, we recognize that the porous PCL bead can induce the new bone formation from the outer surface of bone defect toward the bone marrow cavity through the bead matrix.From the preliminary results, we can suggest that the highly porous PCL beads may be a promising candidate as a bone filler (scaffolding matrix) for the effective bone regeneration.

View Article: PubMed Central - PubMed

Affiliation: Department of Oral and Maxillofacial Surgery, Institute of Health Sciences, Gyeongsang National University School of Medicine, Jinju, 660-702 Korea.

ABSTRACT

Background: For the effective bone regeneration with appropriate pathological/physiological properties, a variety of bone fillers have been adapted as a therapeutic treatment. However, the development of ideal bone fillers is still remained as a big challenge in clinical practice. The main aims of this study are i) fabrication of a highly porous PCL beads; and ii) the estimation of the potential use of the porous PCL beads as a bone filler through preliminary animal study.

Results: The porous PCL beads with size range of 53 ~ 600 μm (425 ~ 500 μm dominantly) are fabricated by a spray/precipitation method using a double nozzle spray and PCL solution (in tetraglycol). The PCL beads show highly porous inner pore structure and the pores are interconnected with outer surface pores. For the preliminary animal study, we recognize that the porous PCL bead can induce the new bone formation from the outer surface of bone defect toward the bone marrow cavity through the bead matrix.

Conclusions: From the preliminary results, we can suggest that the highly porous PCL beads may be a promising candidate as a bone filler (scaffolding matrix) for the effective bone regeneration.

No MeSH data available.


Related in: MedlinePlus

Histological sections of bone defect and surrounding femoral tissue showing the bone regeneration behavior of the control (blank) and porous PCL beads (*, host bone; arrow, new bone; †, PCL beads; H&E staining, x40).
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Fig5: Histological sections of bone defect and surrounding femoral tissue showing the bone regeneration behavior of the control (blank) and porous PCL beads (*, host bone; arrow, new bone; †, PCL beads; H&E staining, x40).

Mentions: A SD rat model was used to estimate the bone regeneration behavior of the porous PCL beads. The rat femoral bone defect was chosen as the orthotopic model for this experiment. Bone defects of ~4 mm diameter were created on a femur using a small tungsten carbide dental bur, and the porous PCL beads were filled into the defect. The blank (w/o any treatment) was also studied as a control group. At implantation, the porous PCL beads were easily applied and formed well-packed structure in the defect site without floating by bleeding. During the breeding, all animals remained in sound health and did not show any wound complications. Figures 5 and 6 show the histological sections (after H&E and MT staining) to compare bone reconstruction behavior in the bone defect filled with and without the porous PCL beads at 4 weeks after surgery. In the PCL bead group, the new bone was regenerated from the outer surface of bone defect toward the bone marrow cavity through the porous PCL beads. However, in the control (blank) group, the new bone was only reconstructed at outer surface of bone defect and the new bone formation in the marrow cavity was not detected. This indicates that the porous PCL bead itself can provide an appropriate environment for ingrowth of a variety of cells related with bone formation, and thus allow bone regeneration in the PCL bead matrix. The regenerated bone was grown along the porous matrix consisted of the PCL beads, suggesting that the beads are stably kept their porous structure without degradation during the bone regeneration and act as a scaffolding matrix which can allow the adhesion of bone-related cells and thus improve osteogenesis [22–24]. At 4 weeks after implantation, the mineralized bone regeneration was also studied using μ-CT. The growth of mineralized bone tissues begun at the outer surface of all bone defects (Figure 7), and the mineralized bone tissue was infiltrated through the PCL bead matrix. This observation was consistent with the result of the histological results, showing that the porous PCL beads can effectively induce the bone regeneration. On the basis of our findings, we can suggest that the highly porous PCL beads fabricated by simple spray/precipitation method may be a candidate as a bone filler for the effective bone regeneration.Figure 5


Effect of porous polycaprolactone beads on bone regeneration: preliminary in vitro and in vivo studies.

Byun JH, Lee HA, Kim TH, Lee JH, Oh SH - Biomater Res (2014)

Histological sections of bone defect and surrounding femoral tissue showing the bone regeneration behavior of the control (blank) and porous PCL beads (*, host bone; arrow, new bone; †, PCL beads; H&E staining, x40).
© Copyright Policy - open-access
Related In: Results  -  Collection

License 1 - License 2
Show All Figures
getmorefigures.php?uid=PMC4552305&req=5

Fig5: Histological sections of bone defect and surrounding femoral tissue showing the bone regeneration behavior of the control (blank) and porous PCL beads (*, host bone; arrow, new bone; †, PCL beads; H&E staining, x40).
Mentions: A SD rat model was used to estimate the bone regeneration behavior of the porous PCL beads. The rat femoral bone defect was chosen as the orthotopic model for this experiment. Bone defects of ~4 mm diameter were created on a femur using a small tungsten carbide dental bur, and the porous PCL beads were filled into the defect. The blank (w/o any treatment) was also studied as a control group. At implantation, the porous PCL beads were easily applied and formed well-packed structure in the defect site without floating by bleeding. During the breeding, all animals remained in sound health and did not show any wound complications. Figures 5 and 6 show the histological sections (after H&E and MT staining) to compare bone reconstruction behavior in the bone defect filled with and without the porous PCL beads at 4 weeks after surgery. In the PCL bead group, the new bone was regenerated from the outer surface of bone defect toward the bone marrow cavity through the porous PCL beads. However, in the control (blank) group, the new bone was only reconstructed at outer surface of bone defect and the new bone formation in the marrow cavity was not detected. This indicates that the porous PCL bead itself can provide an appropriate environment for ingrowth of a variety of cells related with bone formation, and thus allow bone regeneration in the PCL bead matrix. The regenerated bone was grown along the porous matrix consisted of the PCL beads, suggesting that the beads are stably kept their porous structure without degradation during the bone regeneration and act as a scaffolding matrix which can allow the adhesion of bone-related cells and thus improve osteogenesis [22–24]. At 4 weeks after implantation, the mineralized bone regeneration was also studied using μ-CT. The growth of mineralized bone tissues begun at the outer surface of all bone defects (Figure 7), and the mineralized bone tissue was infiltrated through the PCL bead matrix. This observation was consistent with the result of the histological results, showing that the porous PCL beads can effectively induce the bone regeneration. On the basis of our findings, we can suggest that the highly porous PCL beads fabricated by simple spray/precipitation method may be a candidate as a bone filler for the effective bone regeneration.Figure 5

Bottom Line: For the effective bone regeneration with appropriate pathological/physiological properties, a variety of bone fillers have been adapted as a therapeutic treatment.For the preliminary animal study, we recognize that the porous PCL bead can induce the new bone formation from the outer surface of bone defect toward the bone marrow cavity through the bead matrix.From the preliminary results, we can suggest that the highly porous PCL beads may be a promising candidate as a bone filler (scaffolding matrix) for the effective bone regeneration.

View Article: PubMed Central - PubMed

Affiliation: Department of Oral and Maxillofacial Surgery, Institute of Health Sciences, Gyeongsang National University School of Medicine, Jinju, 660-702 Korea.

ABSTRACT

Background: For the effective bone regeneration with appropriate pathological/physiological properties, a variety of bone fillers have been adapted as a therapeutic treatment. However, the development of ideal bone fillers is still remained as a big challenge in clinical practice. The main aims of this study are i) fabrication of a highly porous PCL beads; and ii) the estimation of the potential use of the porous PCL beads as a bone filler through preliminary animal study.

Results: The porous PCL beads with size range of 53 ~ 600 μm (425 ~ 500 μm dominantly) are fabricated by a spray/precipitation method using a double nozzle spray and PCL solution (in tetraglycol). The PCL beads show highly porous inner pore structure and the pores are interconnected with outer surface pores. For the preliminary animal study, we recognize that the porous PCL bead can induce the new bone formation from the outer surface of bone defect toward the bone marrow cavity through the bead matrix.

Conclusions: From the preliminary results, we can suggest that the highly porous PCL beads may be a promising candidate as a bone filler (scaffolding matrix) for the effective bone regeneration.

No MeSH data available.


Related in: MedlinePlus