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Effect of porous polycaprolactone beads on bone regeneration: preliminary in vitro and in vivo studies.

Byun JH, Lee HA, Kim TH, Lee JH, Oh SH - Biomater Res (2014)

Bottom Line: For the effective bone regeneration with appropriate pathological/physiological properties, a variety of bone fillers have been adapted as a therapeutic treatment.For the preliminary animal study, we recognize that the porous PCL bead can induce the new bone formation from the outer surface of bone defect toward the bone marrow cavity through the bead matrix.From the preliminary results, we can suggest that the highly porous PCL beads may be a promising candidate as a bone filler (scaffolding matrix) for the effective bone regeneration.

View Article: PubMed Central - PubMed

Affiliation: Department of Oral and Maxillofacial Surgery, Institute of Health Sciences, Gyeongsang National University School of Medicine, Jinju, 660-702 Korea.

ABSTRACT

Background: For the effective bone regeneration with appropriate pathological/physiological properties, a variety of bone fillers have been adapted as a therapeutic treatment. However, the development of ideal bone fillers is still remained as a big challenge in clinical practice. The main aims of this study are i) fabrication of a highly porous PCL beads; and ii) the estimation of the potential use of the porous PCL beads as a bone filler through preliminary animal study.

Results: The porous PCL beads with size range of 53 ~ 600 μm (425 ~ 500 μm dominantly) are fabricated by a spray/precipitation method using a double nozzle spray and PCL solution (in tetraglycol). The PCL beads show highly porous inner pore structure and the pores are interconnected with outer surface pores. For the preliminary animal study, we recognize that the porous PCL bead can induce the new bone formation from the outer surface of bone defect toward the bone marrow cavity through the bead matrix.

Conclusions: From the preliminary results, we can suggest that the highly porous PCL beads may be a promising candidate as a bone filler (scaffolding matrix) for the effective bone regeneration.

No MeSH data available.


Schematic diagram showing the porous PCL beads fabrication procedure.
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Fig1: Schematic diagram showing the porous PCL beads fabrication procedure.

Mentions: Porous PCL beads were simply prepared by spray/precipitation method using double nozzle spray. PCL pellets were dissolved in tetraglycol at 90°C (15 wt%), and the PCL solution was immediately transferred in a 10 mL syringe. The warm solution was sprayed through a double nozzle spray with N2 purging of 2.5 L/min (outer nozzle) into 50% ethanol solution (coagulation solution) to induce the solidification (precipitation) of PCL solution (Figure 1). Feeding rate of the solution was fixed to 60 mL/h (inner nozzle, using syringe pump). The syringe and double nozzle spray were heated (90°C) using a heating system equipped with heating tape (PID temperature controller, Model, TC130P; heating tape, Model, HT2510; Misung Scientific, Korea) to prevent precipitation of PCL during the process. The distance of tip-to-coagulation solution was 20 cm. The precipitated PCL beads were maintained at coagulation solution for 6 hrs, then the PCL beads were washed out in excess water for 24 hrs to remove residual tetraglycol and ethanol. The PCL beads were obtained by centrifugation and dried in a vacuum oven overnight, and the beads were separated in different size ranges (53 ~ 100, 100 ~ 200, 200 ~ 300, 300 ~ 425, 425 ~ 500, 500 ~ 600 μm) using standard testing sieves (Chunggye Industrial Co., Korea).Figure 1


Effect of porous polycaprolactone beads on bone regeneration: preliminary in vitro and in vivo studies.

Byun JH, Lee HA, Kim TH, Lee JH, Oh SH - Biomater Res (2014)

Schematic diagram showing the porous PCL beads fabrication procedure.
© Copyright Policy - open-access
Related In: Results  -  Collection

License 1 - License 2
Show All Figures
getmorefigures.php?uid=PMC4552305&req=5

Fig1: Schematic diagram showing the porous PCL beads fabrication procedure.
Mentions: Porous PCL beads were simply prepared by spray/precipitation method using double nozzle spray. PCL pellets were dissolved in tetraglycol at 90°C (15 wt%), and the PCL solution was immediately transferred in a 10 mL syringe. The warm solution was sprayed through a double nozzle spray with N2 purging of 2.5 L/min (outer nozzle) into 50% ethanol solution (coagulation solution) to induce the solidification (precipitation) of PCL solution (Figure 1). Feeding rate of the solution was fixed to 60 mL/h (inner nozzle, using syringe pump). The syringe and double nozzle spray were heated (90°C) using a heating system equipped with heating tape (PID temperature controller, Model, TC130P; heating tape, Model, HT2510; Misung Scientific, Korea) to prevent precipitation of PCL during the process. The distance of tip-to-coagulation solution was 20 cm. The precipitated PCL beads were maintained at coagulation solution for 6 hrs, then the PCL beads were washed out in excess water for 24 hrs to remove residual tetraglycol and ethanol. The PCL beads were obtained by centrifugation and dried in a vacuum oven overnight, and the beads were separated in different size ranges (53 ~ 100, 100 ~ 200, 200 ~ 300, 300 ~ 425, 425 ~ 500, 500 ~ 600 μm) using standard testing sieves (Chunggye Industrial Co., Korea).Figure 1

Bottom Line: For the effective bone regeneration with appropriate pathological/physiological properties, a variety of bone fillers have been adapted as a therapeutic treatment.For the preliminary animal study, we recognize that the porous PCL bead can induce the new bone formation from the outer surface of bone defect toward the bone marrow cavity through the bead matrix.From the preliminary results, we can suggest that the highly porous PCL beads may be a promising candidate as a bone filler (scaffolding matrix) for the effective bone regeneration.

View Article: PubMed Central - PubMed

Affiliation: Department of Oral and Maxillofacial Surgery, Institute of Health Sciences, Gyeongsang National University School of Medicine, Jinju, 660-702 Korea.

ABSTRACT

Background: For the effective bone regeneration with appropriate pathological/physiological properties, a variety of bone fillers have been adapted as a therapeutic treatment. However, the development of ideal bone fillers is still remained as a big challenge in clinical practice. The main aims of this study are i) fabrication of a highly porous PCL beads; and ii) the estimation of the potential use of the porous PCL beads as a bone filler through preliminary animal study.

Results: The porous PCL beads with size range of 53 ~ 600 μm (425 ~ 500 μm dominantly) are fabricated by a spray/precipitation method using a double nozzle spray and PCL solution (in tetraglycol). The PCL beads show highly porous inner pore structure and the pores are interconnected with outer surface pores. For the preliminary animal study, we recognize that the porous PCL bead can induce the new bone formation from the outer surface of bone defect toward the bone marrow cavity through the bead matrix.

Conclusions: From the preliminary results, we can suggest that the highly porous PCL beads may be a promising candidate as a bone filler (scaffolding matrix) for the effective bone regeneration.

No MeSH data available.