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Transcriptomic Analysis and Meta-Analysis of Human Granulosa and Cumulus Cells.

Burnik Papler T, Vrtacnik Bokal E, Maver A, Kopitar AN, Lovrečić L - PLoS ONE (2015)

Bottom Line: Specific gene expression in oocytes and its surrounding cumulus (CC) and granulosa (GC) cells is needed for successful folliculogenesis and oocyte maturation.In conclusion, transcriptomes of GC and CC as well as biological functions, are distinctive for each cell subpopulation.By describing novel genes like PROK2 and PNCK, expressed in GC and CC, we upgraded the existing data on human follicular biology.

View Article: PubMed Central - PubMed

Affiliation: Department of Human Reproduction, Division of Obstetrics and Gynaecology, University Medical Centre Ljubljana, Slajmerjeva 2, Ljubljana, Slovenia.

ABSTRACT
Specific gene expression in oocytes and its surrounding cumulus (CC) and granulosa (GC) cells is needed for successful folliculogenesis and oocyte maturation. The aim of the present study was to compare genome-wide gene expression and biological functions of human GC and CC. Individual GC and CC were derived from 37 women undergoing IVF procedures. Gene expression analysis was performed using microarrays, followed by a meta-analysis. Results were validated using quantitative real-time PCR. There were 6029 differentially expressed genes (q < 10-4); of which 650 genes had a log2 FC ≥ 2. After the meta-analysis there were 3156 genes differentially expressed. Among these there were genes that have previously not been reported in human somatic follicular cells, like prokineticin 2 (PROK2), higher expressed in GC, and pregnancy up-regulated nonubiquitous CaM kinase (PNCK), higher expressed in CC. Pathways like inflammatory response and angiogenesis were enriched in GC, whereas in CC, cell differentiation and multicellular organismal development were among enriched pathways. In conclusion, transcriptomes of GC and CC as well as biological functions, are distinctive for each cell subpopulation. By describing novel genes like PROK2 and PNCK, expressed in GC and CC, we upgraded the existing data on human follicular biology.

No MeSH data available.


Related in: MedlinePlus

Top network enriched in cumulus cells (Enrichment score: 41) generated with Ingenuity Pathway Analysis (IPA).The network is associated with cellular development, skeletal and muscular system development and function and tissue development. Up-regulated genes in our study are marked in red colour; the colour intensity of the nodes indicates the degree of up-regulation. Transcripts in grey were not differentially expressed. Genes are represented as nodes, and the biological relationship between two nodes is represented as a line: the plain line indicates direct interaction, the dashed line indicates indirect interaction; the line without arrowhead indicates binding only, the line finishing with a vertical line indicates inhibition; the line with an arrowhead indicates ‘acts on’.
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pone.0136473.g003: Top network enriched in cumulus cells (Enrichment score: 41) generated with Ingenuity Pathway Analysis (IPA).The network is associated with cellular development, skeletal and muscular system development and function and tissue development. Up-regulated genes in our study are marked in red colour; the colour intensity of the nodes indicates the degree of up-regulation. Transcripts in grey were not differentially expressed. Genes are represented as nodes, and the biological relationship between two nodes is represented as a line: the plain line indicates direct interaction, the dashed line indicates indirect interaction; the line without arrowhead indicates binding only, the line finishing with a vertical line indicates inhibition; the line with an arrowhead indicates ‘acts on’.

Mentions: In the CC compartment, the top enriched network was connected with cellular development, skeletal and muscular system development and function and tissue development (Enrichment score: 41) (Fig 3). There were 21 genes of this network highly expressed in CC in our study. The network includes insulin-like growth factor binding protein 5 (IGFBP5) and tenascin C (TNC) genes with functions in extracellular matrix.


Transcriptomic Analysis and Meta-Analysis of Human Granulosa and Cumulus Cells.

Burnik Papler T, Vrtacnik Bokal E, Maver A, Kopitar AN, Lovrečić L - PLoS ONE (2015)

Top network enriched in cumulus cells (Enrichment score: 41) generated with Ingenuity Pathway Analysis (IPA).The network is associated with cellular development, skeletal and muscular system development and function and tissue development. Up-regulated genes in our study are marked in red colour; the colour intensity of the nodes indicates the degree of up-regulation. Transcripts in grey were not differentially expressed. Genes are represented as nodes, and the biological relationship between two nodes is represented as a line: the plain line indicates direct interaction, the dashed line indicates indirect interaction; the line without arrowhead indicates binding only, the line finishing with a vertical line indicates inhibition; the line with an arrowhead indicates ‘acts on’.
© Copyright Policy
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4552299&req=5

pone.0136473.g003: Top network enriched in cumulus cells (Enrichment score: 41) generated with Ingenuity Pathway Analysis (IPA).The network is associated with cellular development, skeletal and muscular system development and function and tissue development. Up-regulated genes in our study are marked in red colour; the colour intensity of the nodes indicates the degree of up-regulation. Transcripts in grey were not differentially expressed. Genes are represented as nodes, and the biological relationship between two nodes is represented as a line: the plain line indicates direct interaction, the dashed line indicates indirect interaction; the line without arrowhead indicates binding only, the line finishing with a vertical line indicates inhibition; the line with an arrowhead indicates ‘acts on’.
Mentions: In the CC compartment, the top enriched network was connected with cellular development, skeletal and muscular system development and function and tissue development (Enrichment score: 41) (Fig 3). There were 21 genes of this network highly expressed in CC in our study. The network includes insulin-like growth factor binding protein 5 (IGFBP5) and tenascin C (TNC) genes with functions in extracellular matrix.

Bottom Line: Specific gene expression in oocytes and its surrounding cumulus (CC) and granulosa (GC) cells is needed for successful folliculogenesis and oocyte maturation.In conclusion, transcriptomes of GC and CC as well as biological functions, are distinctive for each cell subpopulation.By describing novel genes like PROK2 and PNCK, expressed in GC and CC, we upgraded the existing data on human follicular biology.

View Article: PubMed Central - PubMed

Affiliation: Department of Human Reproduction, Division of Obstetrics and Gynaecology, University Medical Centre Ljubljana, Slajmerjeva 2, Ljubljana, Slovenia.

ABSTRACT
Specific gene expression in oocytes and its surrounding cumulus (CC) and granulosa (GC) cells is needed for successful folliculogenesis and oocyte maturation. The aim of the present study was to compare genome-wide gene expression and biological functions of human GC and CC. Individual GC and CC were derived from 37 women undergoing IVF procedures. Gene expression analysis was performed using microarrays, followed by a meta-analysis. Results were validated using quantitative real-time PCR. There were 6029 differentially expressed genes (q < 10-4); of which 650 genes had a log2 FC ≥ 2. After the meta-analysis there were 3156 genes differentially expressed. Among these there were genes that have previously not been reported in human somatic follicular cells, like prokineticin 2 (PROK2), higher expressed in GC, and pregnancy up-regulated nonubiquitous CaM kinase (PNCK), higher expressed in CC. Pathways like inflammatory response and angiogenesis were enriched in GC, whereas in CC, cell differentiation and multicellular organismal development were among enriched pathways. In conclusion, transcriptomes of GC and CC as well as biological functions, are distinctive for each cell subpopulation. By describing novel genes like PROK2 and PNCK, expressed in GC and CC, we upgraded the existing data on human follicular biology.

No MeSH data available.


Related in: MedlinePlus