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Rapid bone regeneration by Escherichia coli-derived recombinant human bone morphogenetic protein-2 loaded on a hydroxyapatite carrier in the rabbit calvarial defect model.

Chung CH, Kim YK, Lee JS, Jung UW, Pang EK, Choi SH - Biomater Res (2015)

Bottom Line: In both histomorphometric and tomographic analyses, the new bone area or volume of the ErhBMP-2/HAP group was significantly greater than that of the HAP and control groups at 2 weeks (p < 0.05).Combining ErhBMP-2 with HAP could significantly promote rapid initial new bone formation.Moreover, HAP graft could increase new bone formation and space maintenance, therefore it might be one of the effective carriers of ErhBMP-2.

View Article: PubMed Central - PubMed

Affiliation: Department of Periodontology, College of Dentistry, Yonsei University, 50 Yonsei-ro Seodaemun-gu, Seoul, 120-752 Republic of Korea.

ABSTRACT

Background: The aim of this study was to determine the osteoconductivity of hydroxyapatite particles (HAP) as a carrier for Escherichia coli-derived recombinant human bone morphogenetic protein-2 (ErhBMP-2). Two 8-mm diameter bicortical calvarial defects were created in each of 20 rabbits. One of each pair of defects was randomly assigned to be filled with HAP only (HAP group) or ErhBMP-2 loaded HAP (ErhBMP-2/HAP group), while the other defect was left untreated (control group). The animals were killed after either 2 weeks (n = 10) or 8 weeks (n = 10) of healing, and histological, histomorphometric, and tomographic analyses were performed.

Results: All experimental sites showed uneventful healing during the postoperative healing period. In both histomorphometric and tomographic analyses, the new bone area or volume of the ErhBMP-2/HAP group was significantly greater than that of the HAP and control groups at 2 weeks (p < 0.05). However, at 8 weeks, no significant difference in new bone area or volume was observed between the ErhBMP-2/HAP and HAP groups. The total augmented area or volume was not significantly different between the ErhBMP-2/HAP and HAP groups at 2 and 8 weeks.

Conclusions: Combining ErhBMP-2 with HAP could significantly promote rapid initial new bone formation. Moreover, HAP graft could increase new bone formation and space maintenance, therefore it might be one of the effective carriers of ErhBMP-2.

No MeSH data available.


Related in: MedlinePlus

Graphs showing tomographic analysis of total augmented volume and new bone volume (mm3). a Two weeks postoperation, b 8 weeks postoperation. NV, new bone volume; TV, total bone volume
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Fig7: Graphs showing tomographic analysis of total augmented volume and new bone volume (mm3). a Two weeks postoperation, b 8 weeks postoperation. NV, new bone volume; TV, total bone volume

Mentions: The overall dimensional topography of the defects and grafts was measured in reconstructed views at 2 and 8 weeks (Figs. 4 and 5). Newly formed bone was gray, while HAP was white because of radiopacity. The total augmented volume and new bone volume of each group were measured using micro-CT (Tables 4 and 5). At 2 and 8 weeks, the total augmented volume was significantly greater in the HAP only and ErhBMP-2/HAP groups than in controls (Fig. 7). However, the total augmented volume did not differ significantly between the HAP only and ErhBMP-2/HAP groups at either 2 or 8 weeks (Table 4). At 2 and 8 weeks, the new bone volume was significantly greater in the HAP only and ErhBMP-2/HAP groups than in controls (Fig. 7). At 2 weeks, the new bone volume was significantly greater in the ErhBMP-2/HAP group than in the other groups. At 8 weeks, the difference in new bone volume between the HAP only and ErhBMP-2/HAP groups was not significant (Table 5).Table 4


Rapid bone regeneration by Escherichia coli-derived recombinant human bone morphogenetic protein-2 loaded on a hydroxyapatite carrier in the rabbit calvarial defect model.

Chung CH, Kim YK, Lee JS, Jung UW, Pang EK, Choi SH - Biomater Res (2015)

Graphs showing tomographic analysis of total augmented volume and new bone volume (mm3). a Two weeks postoperation, b 8 weeks postoperation. NV, new bone volume; TV, total bone volume
© Copyright Policy - open-access
Related In: Results  -  Collection

License 1 - License 2
Show All Figures
getmorefigures.php?uid=PMC4552284&req=5

Fig7: Graphs showing tomographic analysis of total augmented volume and new bone volume (mm3). a Two weeks postoperation, b 8 weeks postoperation. NV, new bone volume; TV, total bone volume
Mentions: The overall dimensional topography of the defects and grafts was measured in reconstructed views at 2 and 8 weeks (Figs. 4 and 5). Newly formed bone was gray, while HAP was white because of radiopacity. The total augmented volume and new bone volume of each group were measured using micro-CT (Tables 4 and 5). At 2 and 8 weeks, the total augmented volume was significantly greater in the HAP only and ErhBMP-2/HAP groups than in controls (Fig. 7). However, the total augmented volume did not differ significantly between the HAP only and ErhBMP-2/HAP groups at either 2 or 8 weeks (Table 4). At 2 and 8 weeks, the new bone volume was significantly greater in the HAP only and ErhBMP-2/HAP groups than in controls (Fig. 7). At 2 weeks, the new bone volume was significantly greater in the ErhBMP-2/HAP group than in the other groups. At 8 weeks, the difference in new bone volume between the HAP only and ErhBMP-2/HAP groups was not significant (Table 5).Table 4

Bottom Line: In both histomorphometric and tomographic analyses, the new bone area or volume of the ErhBMP-2/HAP group was significantly greater than that of the HAP and control groups at 2 weeks (p < 0.05).Combining ErhBMP-2 with HAP could significantly promote rapid initial new bone formation.Moreover, HAP graft could increase new bone formation and space maintenance, therefore it might be one of the effective carriers of ErhBMP-2.

View Article: PubMed Central - PubMed

Affiliation: Department of Periodontology, College of Dentistry, Yonsei University, 50 Yonsei-ro Seodaemun-gu, Seoul, 120-752 Republic of Korea.

ABSTRACT

Background: The aim of this study was to determine the osteoconductivity of hydroxyapatite particles (HAP) as a carrier for Escherichia coli-derived recombinant human bone morphogenetic protein-2 (ErhBMP-2). Two 8-mm diameter bicortical calvarial defects were created in each of 20 rabbits. One of each pair of defects was randomly assigned to be filled with HAP only (HAP group) or ErhBMP-2 loaded HAP (ErhBMP-2/HAP group), while the other defect was left untreated (control group). The animals were killed after either 2 weeks (n = 10) or 8 weeks (n = 10) of healing, and histological, histomorphometric, and tomographic analyses were performed.

Results: All experimental sites showed uneventful healing during the postoperative healing period. In both histomorphometric and tomographic analyses, the new bone area or volume of the ErhBMP-2/HAP group was significantly greater than that of the HAP and control groups at 2 weeks (p < 0.05). However, at 8 weeks, no significant difference in new bone area or volume was observed between the ErhBMP-2/HAP and HAP groups. The total augmented area or volume was not significantly different between the ErhBMP-2/HAP and HAP groups at 2 and 8 weeks.

Conclusions: Combining ErhBMP-2 with HAP could significantly promote rapid initial new bone formation. Moreover, HAP graft could increase new bone formation and space maintenance, therefore it might be one of the effective carriers of ErhBMP-2.

No MeSH data available.


Related in: MedlinePlus