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Rapid bone regeneration by Escherichia coli-derived recombinant human bone morphogenetic protein-2 loaded on a hydroxyapatite carrier in the rabbit calvarial defect model.

Chung CH, Kim YK, Lee JS, Jung UW, Pang EK, Choi SH - Biomater Res (2015)

Bottom Line: In both histomorphometric and tomographic analyses, the new bone area or volume of the ErhBMP-2/HAP group was significantly greater than that of the HAP and control groups at 2 weeks (p < 0.05).The total augmented area or volume was not significantly different between the ErhBMP-2/HAP and HAP groups at 2 and 8 weeks.Combining ErhBMP-2 with HAP could significantly promote rapid initial new bone formation.

View Article: PubMed Central - PubMed

Affiliation: Department of Periodontology, College of Dentistry, Yonsei University, 50 Yonsei-ro Seodaemun-gu, Seoul, 120-752 Republic of Korea.

ABSTRACT

Background: The aim of this study was to determine the osteoconductivity of hydroxyapatite particles (HAP) as a carrier for Escherichia coli-derived recombinant human bone morphogenetic protein-2 (ErhBMP-2). Two 8-mm diameter bicortical calvarial defects were created in each of 20 rabbits. One of each pair of defects was randomly assigned to be filled with HAP only (HAP group) or ErhBMP-2 loaded HAP (ErhBMP-2/HAP group), while the other defect was left untreated (control group). The animals were killed after either 2 weeks (n = 10) or 8 weeks (n = 10) of healing, and histological, histomorphometric, and tomographic analyses were performed.

Results: All experimental sites showed uneventful healing during the postoperative healing period. In both histomorphometric and tomographic analyses, the new bone area or volume of the ErhBMP-2/HAP group was significantly greater than that of the HAP and control groups at 2 weeks (p < 0.05). However, at 8 weeks, no significant difference in new bone area or volume was observed between the ErhBMP-2/HAP and HAP groups. The total augmented area or volume was not significantly different between the ErhBMP-2/HAP and HAP groups at 2 and 8 weeks.

Conclusions: Combining ErhBMP-2 with HAP could significantly promote rapid initial new bone formation. Moreover, HAP graft could increase new bone formation and space maintenance, therefore it might be one of the effective carriers of ErhBMP-2.

No MeSH data available.


Related in: MedlinePlus

Representative coronally sectioned micro-computed tomography images at 8 weeks postoperation. a ErhBMP-2/HAP group, b HA only group
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Fig5: Representative coronally sectioned micro-computed tomography images at 8 weeks postoperation. a ErhBMP-2/HAP group, b HA only group

Mentions: Specimens were scanned using a microcomputed tomography (micro-CT) system (SkyScans1072, SkyScan, Aartselaar, Belgium) at a resolution of 18 μm (100 kV and 100 μA) (Figs. 4 and 5). The scanned sets of data were processed in DICOM format, and the sum of the cross-sectional view was used to reconstruct the area of interest [15]. The overall dimensional topography of the recipient beds was measured in the reconstructed views.Fig. 4


Rapid bone regeneration by Escherichia coli-derived recombinant human bone morphogenetic protein-2 loaded on a hydroxyapatite carrier in the rabbit calvarial defect model.

Chung CH, Kim YK, Lee JS, Jung UW, Pang EK, Choi SH - Biomater Res (2015)

Representative coronally sectioned micro-computed tomography images at 8 weeks postoperation. a ErhBMP-2/HAP group, b HA only group
© Copyright Policy - open-access
Related In: Results  -  Collection

License 1 - License 2
Show All Figures
getmorefigures.php?uid=PMC4552284&req=5

Fig5: Representative coronally sectioned micro-computed tomography images at 8 weeks postoperation. a ErhBMP-2/HAP group, b HA only group
Mentions: Specimens were scanned using a microcomputed tomography (micro-CT) system (SkyScans1072, SkyScan, Aartselaar, Belgium) at a resolution of 18 μm (100 kV and 100 μA) (Figs. 4 and 5). The scanned sets of data were processed in DICOM format, and the sum of the cross-sectional view was used to reconstruct the area of interest [15]. The overall dimensional topography of the recipient beds was measured in the reconstructed views.Fig. 4

Bottom Line: In both histomorphometric and tomographic analyses, the new bone area or volume of the ErhBMP-2/HAP group was significantly greater than that of the HAP and control groups at 2 weeks (p < 0.05).The total augmented area or volume was not significantly different between the ErhBMP-2/HAP and HAP groups at 2 and 8 weeks.Combining ErhBMP-2 with HAP could significantly promote rapid initial new bone formation.

View Article: PubMed Central - PubMed

Affiliation: Department of Periodontology, College of Dentistry, Yonsei University, 50 Yonsei-ro Seodaemun-gu, Seoul, 120-752 Republic of Korea.

ABSTRACT

Background: The aim of this study was to determine the osteoconductivity of hydroxyapatite particles (HAP) as a carrier for Escherichia coli-derived recombinant human bone morphogenetic protein-2 (ErhBMP-2). Two 8-mm diameter bicortical calvarial defects were created in each of 20 rabbits. One of each pair of defects was randomly assigned to be filled with HAP only (HAP group) or ErhBMP-2 loaded HAP (ErhBMP-2/HAP group), while the other defect was left untreated (control group). The animals were killed after either 2 weeks (n = 10) or 8 weeks (n = 10) of healing, and histological, histomorphometric, and tomographic analyses were performed.

Results: All experimental sites showed uneventful healing during the postoperative healing period. In both histomorphometric and tomographic analyses, the new bone area or volume of the ErhBMP-2/HAP group was significantly greater than that of the HAP and control groups at 2 weeks (p < 0.05). However, at 8 weeks, no significant difference in new bone area or volume was observed between the ErhBMP-2/HAP and HAP groups. The total augmented area or volume was not significantly different between the ErhBMP-2/HAP and HAP groups at 2 and 8 weeks.

Conclusions: Combining ErhBMP-2 with HAP could significantly promote rapid initial new bone formation. Moreover, HAP graft could increase new bone formation and space maintenance, therefore it might be one of the effective carriers of ErhBMP-2.

No MeSH data available.


Related in: MedlinePlus