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Rapid bone regeneration by Escherichia coli-derived recombinant human bone morphogenetic protein-2 loaded on a hydroxyapatite carrier in the rabbit calvarial defect model.

Chung CH, Kim YK, Lee JS, Jung UW, Pang EK, Choi SH - Biomater Res (2015)

Bottom Line: In both histomorphometric and tomographic analyses, the new bone area or volume of the ErhBMP-2/HAP group was significantly greater than that of the HAP and control groups at 2 weeks (p < 0.05).Combining ErhBMP-2 with HAP could significantly promote rapid initial new bone formation.Moreover, HAP graft could increase new bone formation and space maintenance, therefore it might be one of the effective carriers of ErhBMP-2.

View Article: PubMed Central - PubMed

Affiliation: Department of Periodontology, College of Dentistry, Yonsei University, 50 Yonsei-ro Seodaemun-gu, Seoul, 120-752 Republic of Korea.

ABSTRACT

Background: The aim of this study was to determine the osteoconductivity of hydroxyapatite particles (HAP) as a carrier for Escherichia coli-derived recombinant human bone morphogenetic protein-2 (ErhBMP-2). Two 8-mm diameter bicortical calvarial defects were created in each of 20 rabbits. One of each pair of defects was randomly assigned to be filled with HAP only (HAP group) or ErhBMP-2 loaded HAP (ErhBMP-2/HAP group), while the other defect was left untreated (control group). The animals were killed after either 2 weeks (n = 10) or 8 weeks (n = 10) of healing, and histological, histomorphometric, and tomographic analyses were performed.

Results: All experimental sites showed uneventful healing during the postoperative healing period. In both histomorphometric and tomographic analyses, the new bone area or volume of the ErhBMP-2/HAP group was significantly greater than that of the HAP and control groups at 2 weeks (p < 0.05). However, at 8 weeks, no significant difference in new bone area or volume was observed between the ErhBMP-2/HAP and HAP groups. The total augmented area or volume was not significantly different between the ErhBMP-2/HAP and HAP groups at 2 and 8 weeks.

Conclusions: Combining ErhBMP-2 with HAP could significantly promote rapid initial new bone formation. Moreover, HAP graft could increase new bone formation and space maintenance, therefore it might be one of the effective carriers of ErhBMP-2.

No MeSH data available.


Related in: MedlinePlus

Representative photomicrographs obtained at 2 weeks postoperation. a Control, b HAP only, c ErhBMP-2/HAP (hematoxylin and eosin, ×40). Arrowheads = defect margin
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Fig2: Representative photomicrographs obtained at 2 weeks postoperation. a Control, b HAP only, c ErhBMP-2/HAP (hematoxylin and eosin, ×40). Arrowheads = defect margin

Mentions: Histomorphometric data for the following parameters were obtained with an automated image-analysis system (Image-Pro Plus, Media Cybernetics, Silver Spring, MD, USA; Figs. 2 and 3):Fig. 2


Rapid bone regeneration by Escherichia coli-derived recombinant human bone morphogenetic protein-2 loaded on a hydroxyapatite carrier in the rabbit calvarial defect model.

Chung CH, Kim YK, Lee JS, Jung UW, Pang EK, Choi SH - Biomater Res (2015)

Representative photomicrographs obtained at 2 weeks postoperation. a Control, b HAP only, c ErhBMP-2/HAP (hematoxylin and eosin, ×40). Arrowheads = defect margin
© Copyright Policy - open-access
Related In: Results  -  Collection

License 1 - License 2
Show All Figures
getmorefigures.php?uid=PMC4552284&req=5

Fig2: Representative photomicrographs obtained at 2 weeks postoperation. a Control, b HAP only, c ErhBMP-2/HAP (hematoxylin and eosin, ×40). Arrowheads = defect margin
Mentions: Histomorphometric data for the following parameters were obtained with an automated image-analysis system (Image-Pro Plus, Media Cybernetics, Silver Spring, MD, USA; Figs. 2 and 3):Fig. 2

Bottom Line: In both histomorphometric and tomographic analyses, the new bone area or volume of the ErhBMP-2/HAP group was significantly greater than that of the HAP and control groups at 2 weeks (p < 0.05).Combining ErhBMP-2 with HAP could significantly promote rapid initial new bone formation.Moreover, HAP graft could increase new bone formation and space maintenance, therefore it might be one of the effective carriers of ErhBMP-2.

View Article: PubMed Central - PubMed

Affiliation: Department of Periodontology, College of Dentistry, Yonsei University, 50 Yonsei-ro Seodaemun-gu, Seoul, 120-752 Republic of Korea.

ABSTRACT

Background: The aim of this study was to determine the osteoconductivity of hydroxyapatite particles (HAP) as a carrier for Escherichia coli-derived recombinant human bone morphogenetic protein-2 (ErhBMP-2). Two 8-mm diameter bicortical calvarial defects were created in each of 20 rabbits. One of each pair of defects was randomly assigned to be filled with HAP only (HAP group) or ErhBMP-2 loaded HAP (ErhBMP-2/HAP group), while the other defect was left untreated (control group). The animals were killed after either 2 weeks (n = 10) or 8 weeks (n = 10) of healing, and histological, histomorphometric, and tomographic analyses were performed.

Results: All experimental sites showed uneventful healing during the postoperative healing period. In both histomorphometric and tomographic analyses, the new bone area or volume of the ErhBMP-2/HAP group was significantly greater than that of the HAP and control groups at 2 weeks (p < 0.05). However, at 8 weeks, no significant difference in new bone area or volume was observed between the ErhBMP-2/HAP and HAP groups. The total augmented area or volume was not significantly different between the ErhBMP-2/HAP and HAP groups at 2 and 8 weeks.

Conclusions: Combining ErhBMP-2 with HAP could significantly promote rapid initial new bone formation. Moreover, HAP graft could increase new bone formation and space maintenance, therefore it might be one of the effective carriers of ErhBMP-2.

No MeSH data available.


Related in: MedlinePlus