Limits...
Combined Efficacy of Gallic Acid and MiADMSA with Limited Beneficial Effects Over MiADMSA Against Arsenic-induced Oxidative Stress in Mouse.

Pachauri V, Flora S - Biochem Insights (2015)

Bottom Line: The study suggests that (1) gallic acid in presence of MiADMSA is only moderately beneficial against arsenic, (2) monotherapy with gallic acid is more effective than in combination with MiADMSA after arsenic exposure in reducing oxidative injury, and (3) MiADMSA monotherapy as reported previously provides significant therapeutic efficacy against arsenic.Thus, based on the present results, we conclude that gallic acid is effective against arsenic-induced oxidative stress but provides limited additional beneficial effects when administered in combination with MiADMSA.We still recommend that lower doses of gallic acid be evaluated both individually and in combination with MiADMSA, as it might not exhibit the shortcomings we observed with higher doses in this study.

View Article: PubMed Central - PubMed

Affiliation: Division of Regulatory Toxicology, Defence Research and Development Establishment, Gwalior, India.

ABSTRACT
Gallic acid is an organic acid known for its antioxidant and anticancer properties. The present study is focused on evaluating the role of gallic acid in providing better therapeutic outcomes against arsenic-induced toxicity. Animals pre-exposed to arsenic were treated with monoisoamyl meso-2,3-dimercaptosuccinic acid (MiADMSA), a new chelating drug, alone and in combination with gallic acid, consecutively for 10 days. The study suggests that (1) gallic acid in presence of MiADMSA is only moderately beneficial against arsenic, (2) monotherapy with gallic acid is more effective than in combination with MiADMSA after arsenic exposure in reducing oxidative injury, and (3) MiADMSA monotherapy as reported previously provides significant therapeutic efficacy against arsenic. Thus, based on the present results, we conclude that gallic acid is effective against arsenic-induced oxidative stress but provides limited additional beneficial effects when administered in combination with MiADMSA. We still recommend that lower doses of gallic acid be evaluated both individually and in combination with MiADMSA, as it might not exhibit the shortcomings we observed with higher doses in this study.

No MeSH data available.


Related in: MedlinePlus

Effect of MiADMSA alone or in combination with gallic acid on urinary levels of 8-OHdG in arsenic exposed mice. Groups: 1, control (no treatment); 2, MiADMSA; 3, gallic acid; 4, arsenic; 5, arsenic + MiADMSA; 6, arsenic + gallic acid; 7, arsenic + gallic acid + MiADMSA.Notes: Values are mean ± SE; n = 8. *,†,‡,§Values with matching symbol notations in each graph are not statistically significant at 5% level of probability.Abbreviations and units: 8-OHdG, 8-oxo-7,8-dihydro-2 deoxyguanosine (ng/dl urinary volume).
© Copyright Policy - open-access
Related In: Results  -  Collection


getmorefigures.php?uid=PMC4552275&req=5

f5-bci-8-2015-001: Effect of MiADMSA alone or in combination with gallic acid on urinary levels of 8-OHdG in arsenic exposed mice. Groups: 1, control (no treatment); 2, MiADMSA; 3, gallic acid; 4, arsenic; 5, arsenic + MiADMSA; 6, arsenic + gallic acid; 7, arsenic + gallic acid + MiADMSA.Notes: Values are mean ± SE; n = 8. *,†,‡,§Values with matching symbol notations in each graph are not statistically significant at 5% level of probability.Abbreviations and units: 8-OHdG, 8-oxo-7,8-dihydro-2 deoxyguanosine (ng/dl urinary volume).

Mentions: 8-OHdG is an oxidized derivative of deoxyguanosine. It is one of the major products of DNA oxidation. The concentration of 8-OHdG within a cell is used as a measure of oxidative stress. Its concentration in DNA is, in fact, a quantitative analysis of the degree of DNA damage that an organism has undergone. We measured its level in urine. A significant increase in the 8-OHdG level was noted when arsenic and gallic acid were administered alone (Fig. 5). The level was significantly reduced on monotherapy with MiADMSA in arsenic pre-exposed animals, while gallic acid administration alone and in combination with MiADMSA did not have any effect on the elevated 8-OHdG level.


Combined Efficacy of Gallic Acid and MiADMSA with Limited Beneficial Effects Over MiADMSA Against Arsenic-induced Oxidative Stress in Mouse.

Pachauri V, Flora S - Biochem Insights (2015)

Effect of MiADMSA alone or in combination with gallic acid on urinary levels of 8-OHdG in arsenic exposed mice. Groups: 1, control (no treatment); 2, MiADMSA; 3, gallic acid; 4, arsenic; 5, arsenic + MiADMSA; 6, arsenic + gallic acid; 7, arsenic + gallic acid + MiADMSA.Notes: Values are mean ± SE; n = 8. *,†,‡,§Values with matching symbol notations in each graph are not statistically significant at 5% level of probability.Abbreviations and units: 8-OHdG, 8-oxo-7,8-dihydro-2 deoxyguanosine (ng/dl urinary volume).
© Copyright Policy - open-access
Related In: Results  -  Collection

Show All Figures
getmorefigures.php?uid=PMC4552275&req=5

f5-bci-8-2015-001: Effect of MiADMSA alone or in combination with gallic acid on urinary levels of 8-OHdG in arsenic exposed mice. Groups: 1, control (no treatment); 2, MiADMSA; 3, gallic acid; 4, arsenic; 5, arsenic + MiADMSA; 6, arsenic + gallic acid; 7, arsenic + gallic acid + MiADMSA.Notes: Values are mean ± SE; n = 8. *,†,‡,§Values with matching symbol notations in each graph are not statistically significant at 5% level of probability.Abbreviations and units: 8-OHdG, 8-oxo-7,8-dihydro-2 deoxyguanosine (ng/dl urinary volume).
Mentions: 8-OHdG is an oxidized derivative of deoxyguanosine. It is one of the major products of DNA oxidation. The concentration of 8-OHdG within a cell is used as a measure of oxidative stress. Its concentration in DNA is, in fact, a quantitative analysis of the degree of DNA damage that an organism has undergone. We measured its level in urine. A significant increase in the 8-OHdG level was noted when arsenic and gallic acid were administered alone (Fig. 5). The level was significantly reduced on monotherapy with MiADMSA in arsenic pre-exposed animals, while gallic acid administration alone and in combination with MiADMSA did not have any effect on the elevated 8-OHdG level.

Bottom Line: The study suggests that (1) gallic acid in presence of MiADMSA is only moderately beneficial against arsenic, (2) monotherapy with gallic acid is more effective than in combination with MiADMSA after arsenic exposure in reducing oxidative injury, and (3) MiADMSA monotherapy as reported previously provides significant therapeutic efficacy against arsenic.Thus, based on the present results, we conclude that gallic acid is effective against arsenic-induced oxidative stress but provides limited additional beneficial effects when administered in combination with MiADMSA.We still recommend that lower doses of gallic acid be evaluated both individually and in combination with MiADMSA, as it might not exhibit the shortcomings we observed with higher doses in this study.

View Article: PubMed Central - PubMed

Affiliation: Division of Regulatory Toxicology, Defence Research and Development Establishment, Gwalior, India.

ABSTRACT
Gallic acid is an organic acid known for its antioxidant and anticancer properties. The present study is focused on evaluating the role of gallic acid in providing better therapeutic outcomes against arsenic-induced toxicity. Animals pre-exposed to arsenic were treated with monoisoamyl meso-2,3-dimercaptosuccinic acid (MiADMSA), a new chelating drug, alone and in combination with gallic acid, consecutively for 10 days. The study suggests that (1) gallic acid in presence of MiADMSA is only moderately beneficial against arsenic, (2) monotherapy with gallic acid is more effective than in combination with MiADMSA after arsenic exposure in reducing oxidative injury, and (3) MiADMSA monotherapy as reported previously provides significant therapeutic efficacy against arsenic. Thus, based on the present results, we conclude that gallic acid is effective against arsenic-induced oxidative stress but provides limited additional beneficial effects when administered in combination with MiADMSA. We still recommend that lower doses of gallic acid be evaluated both individually and in combination with MiADMSA, as it might not exhibit the shortcomings we observed with higher doses in this study.

No MeSH data available.


Related in: MedlinePlus