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Combined Efficacy of Gallic Acid and MiADMSA with Limited Beneficial Effects Over MiADMSA Against Arsenic-induced Oxidative Stress in Mouse.

Pachauri V, Flora S - Biochem Insights (2015)

Bottom Line: Gallic acid is an organic acid known for its antioxidant and anticancer properties.Thus, based on the present results, we conclude that gallic acid is effective against arsenic-induced oxidative stress but provides limited additional beneficial effects when administered in combination with MiADMSA.We still recommend that lower doses of gallic acid be evaluated both individually and in combination with MiADMSA, as it might not exhibit the shortcomings we observed with higher doses in this study.

View Article: PubMed Central - PubMed

Affiliation: Division of Regulatory Toxicology, Defence Research and Development Establishment, Gwalior, India.

ABSTRACT
Gallic acid is an organic acid known for its antioxidant and anticancer properties. The present study is focused on evaluating the role of gallic acid in providing better therapeutic outcomes against arsenic-induced toxicity. Animals pre-exposed to arsenic were treated with monoisoamyl meso-2,3-dimercaptosuccinic acid (MiADMSA), a new chelating drug, alone and in combination with gallic acid, consecutively for 10 days. The study suggests that (1) gallic acid in presence of MiADMSA is only moderately beneficial against arsenic, (2) monotherapy with gallic acid is more effective than in combination with MiADMSA after arsenic exposure in reducing oxidative injury, and (3) MiADMSA monotherapy as reported previously provides significant therapeutic efficacy against arsenic. Thus, based on the present results, we conclude that gallic acid is effective against arsenic-induced oxidative stress but provides limited additional beneficial effects when administered in combination with MiADMSA. We still recommend that lower doses of gallic acid be evaluated both individually and in combination with MiADMSA, as it might not exhibit the shortcomings we observed with higher doses in this study.

No MeSH data available.


Related in: MedlinePlus

Effect of MiADMSA alone or in combination with gallic acid on brain ROS, GSH, and TBARS levels in arsenic-exposed mice. Groups: 1, control (no treatment); 2, MiADMSA; 3, gallic acid; 4, arsenic; 5, arsenic + MiADMSA; 6, arsenic + gallic acid; 7, arsenic + gallic acid + MiADMSA.Notes: Values are mean ± SE; n = 8. *,†,‡Values with matching symbol notations in each graph are not statistically significant at 5% level of probability.Abbreviations and units: ROS, reactive oxygen species (in fluorescence intensity units); GSH, reduced glutathione (mg/mL); TBARS, thiobarbituric reactive substances expressed (nmoles of TBARS/gm of tissue).
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f3-bci-8-2015-001: Effect of MiADMSA alone or in combination with gallic acid on brain ROS, GSH, and TBARS levels in arsenic-exposed mice. Groups: 1, control (no treatment); 2, MiADMSA; 3, gallic acid; 4, arsenic; 5, arsenic + MiADMSA; 6, arsenic + gallic acid; 7, arsenic + gallic acid + MiADMSA.Notes: Values are mean ± SE; n = 8. *,†,‡Values with matching symbol notations in each graph are not statistically significant at 5% level of probability.Abbreviations and units: ROS, reactive oxygen species (in fluorescence intensity units); GSH, reduced glutathione (mg/mL); TBARS, thiobarbituric reactive substances expressed (nmoles of TBARS/gm of tissue).

Mentions: Brain ROS level increased on arsenic exposure. It was, however, interesting to note that the administration of gallic acid in normal animals also led to an increase in ROS level. This might be due to fact that we used a higher dose of gallic acid. It will be interesting to see whether such an effect persists at lower doses. Brain GSH level decreased significantly on arsenic exposure (Fig. 3). No effect of any of the treatment on the brain TBARS level was noted where the end product of lipid peroxidation is MDA. Treatment with combined administration of gallic acid and MiADMSA produced a significant recovery in brain GSH level, while no effect on brain ROS level was noted.


Combined Efficacy of Gallic Acid and MiADMSA with Limited Beneficial Effects Over MiADMSA Against Arsenic-induced Oxidative Stress in Mouse.

Pachauri V, Flora S - Biochem Insights (2015)

Effect of MiADMSA alone or in combination with gallic acid on brain ROS, GSH, and TBARS levels in arsenic-exposed mice. Groups: 1, control (no treatment); 2, MiADMSA; 3, gallic acid; 4, arsenic; 5, arsenic + MiADMSA; 6, arsenic + gallic acid; 7, arsenic + gallic acid + MiADMSA.Notes: Values are mean ± SE; n = 8. *,†,‡Values with matching symbol notations in each graph are not statistically significant at 5% level of probability.Abbreviations and units: ROS, reactive oxygen species (in fluorescence intensity units); GSH, reduced glutathione (mg/mL); TBARS, thiobarbituric reactive substances expressed (nmoles of TBARS/gm of tissue).
© Copyright Policy - open-access
Related In: Results  -  Collection

Show All Figures
getmorefigures.php?uid=PMC4552275&req=5

f3-bci-8-2015-001: Effect of MiADMSA alone or in combination with gallic acid on brain ROS, GSH, and TBARS levels in arsenic-exposed mice. Groups: 1, control (no treatment); 2, MiADMSA; 3, gallic acid; 4, arsenic; 5, arsenic + MiADMSA; 6, arsenic + gallic acid; 7, arsenic + gallic acid + MiADMSA.Notes: Values are mean ± SE; n = 8. *,†,‡Values with matching symbol notations in each graph are not statistically significant at 5% level of probability.Abbreviations and units: ROS, reactive oxygen species (in fluorescence intensity units); GSH, reduced glutathione (mg/mL); TBARS, thiobarbituric reactive substances expressed (nmoles of TBARS/gm of tissue).
Mentions: Brain ROS level increased on arsenic exposure. It was, however, interesting to note that the administration of gallic acid in normal animals also led to an increase in ROS level. This might be due to fact that we used a higher dose of gallic acid. It will be interesting to see whether such an effect persists at lower doses. Brain GSH level decreased significantly on arsenic exposure (Fig. 3). No effect of any of the treatment on the brain TBARS level was noted where the end product of lipid peroxidation is MDA. Treatment with combined administration of gallic acid and MiADMSA produced a significant recovery in brain GSH level, while no effect on brain ROS level was noted.

Bottom Line: Gallic acid is an organic acid known for its antioxidant and anticancer properties.Thus, based on the present results, we conclude that gallic acid is effective against arsenic-induced oxidative stress but provides limited additional beneficial effects when administered in combination with MiADMSA.We still recommend that lower doses of gallic acid be evaluated both individually and in combination with MiADMSA, as it might not exhibit the shortcomings we observed with higher doses in this study.

View Article: PubMed Central - PubMed

Affiliation: Division of Regulatory Toxicology, Defence Research and Development Establishment, Gwalior, India.

ABSTRACT
Gallic acid is an organic acid known for its antioxidant and anticancer properties. The present study is focused on evaluating the role of gallic acid in providing better therapeutic outcomes against arsenic-induced toxicity. Animals pre-exposed to arsenic were treated with monoisoamyl meso-2,3-dimercaptosuccinic acid (MiADMSA), a new chelating drug, alone and in combination with gallic acid, consecutively for 10 days. The study suggests that (1) gallic acid in presence of MiADMSA is only moderately beneficial against arsenic, (2) monotherapy with gallic acid is more effective than in combination with MiADMSA after arsenic exposure in reducing oxidative injury, and (3) MiADMSA monotherapy as reported previously provides significant therapeutic efficacy against arsenic. Thus, based on the present results, we conclude that gallic acid is effective against arsenic-induced oxidative stress but provides limited additional beneficial effects when administered in combination with MiADMSA. We still recommend that lower doses of gallic acid be evaluated both individually and in combination with MiADMSA, as it might not exhibit the shortcomings we observed with higher doses in this study.

No MeSH data available.


Related in: MedlinePlus