Limits...
Photo-thermal effect enhances the efficiency of radiotherapy using Arg-Gly-Asp peptides-conjugated gold nanorods that target αvβ3 in melanoma cancer cells.

Li P, Shi YW, Li BX, Xu WC, Shi ZL, Zhou C, Fu S - J Nanobiotechnology (2015)

Bottom Line: Compared to other treatments, flow cytometric analysis indicated that RT + NIR + RGD-GNRs increased apoptosis (p < 0.001) and decreased the proportion of cells in the more radioresistant S phase (p = 0.014).Treated with NIR + RGD-GNRs, the radiosensitivity was also significantly enhanced (DMFSF2: 1.41).And it would greatly benefit the therapeutic effects of refractory or recurrent malignant cancers.

View Article: PubMed Central - PubMed

Affiliation: Department of Radiation Oncology, Shanghai Proton and Heavy Ion Center, Fudan University Cancer Hospital, Shanghai, People's Republic of China. liping529@gmail.com.

ABSTRACT

Background: Thermotherapy has been known to be one of the most effective adjuvants to radiotherapy (RT) in cancer treatment, but it is not widely implemented clinically due to some limitations, such as, inadequate temperature concentrations to the tumor tissue, nonspecific and non-uniform distribution of heat. So we constructed arginine-glycine-aspartate peptides-conjugated gold nanorods (RGD-GNRs) that target the alpha(v) beta(3) Integrin (αvβ3) and investigate whether the photo-thermal effect of RGD-GNRs by near infrared radiation (NIR) could enhance the efficiency of RT in melanoma cancer cells.

Results: RGD-GNRs could be seen both on the surface of the cell membranes and cytoplasm of A375 cells with high expression of αvβ3. After exposed to 808 nm NIR, RGD-GNRs with various concentrations could be rapidly heated up. Compared to other treatments, flow cytometric analysis indicated that RT + NIR + RGD-GNRs increased apoptosis (p < 0.001) and decreased the proportion of cells in the more radioresistant S phase (p = 0.014). Treated with NIR + RGD-GNRs, the radiosensitivity was also significantly enhanced (DMFSF2: 1.41).

Conclusion: Results of the current study showed the feasibility of using RGD-GNRs for synergetic RT with photo-thermal therapy. And it would greatly benefit the therapeutic effects of refractory or recurrent malignant cancers.

No MeSH data available.


Related in: MedlinePlus

The apoptosis of the A375 cells after NIR or/and RGD-GNRs treatment. A375 cells were treated with RGD-GNRs or/and irradiation for 1 h prior to irradiation (6 mV X-rays with a dose of 4 Gy). The cells were stained with Annexin V and propidium iodide, and apoptosis was analyzed by flow cytometry after 24 h of treatment.
© Copyright Policy - OpenAccess
Related In: Results  -  Collection

License 1 - License 2
getmorefigures.php?uid=PMC4552250&req=5

Fig5: The apoptosis of the A375 cells after NIR or/and RGD-GNRs treatment. A375 cells were treated with RGD-GNRs or/and irradiation for 1 h prior to irradiation (6 mV X-rays with a dose of 4 Gy). The cells were stained with Annexin V and propidium iodide, and apoptosis was analyzed by flow cytometry after 24 h of treatment.

Mentions: Considering that hyperthermia-induced radiosensitization is known to be associated with more sensitive to S phase cells, which are resistant to radiation. It was determined whether a cell cycle rationale could be used to account for the greater efficacy of the photo-thermal treatment and radiotherapy combination (Fig. 5). As shown in Fig. 6, after treatment with RGD-GNRs and NIR for 24 h, there were a significant accumulation of cells in G2/M phase (p = 0.022, compared with other treatment groups) and a drastic decrease of S phase cells (p = 0.014, compared with other treatment groups), No significant difference can be seen in G0/G1 phase cells among the three groups. So hyperthermia may sensitize cells to radiation by regulating the cell cycle. The combination of thermotherapy and radiotherapy increases damage to all phases of tumor cells, but the killing effects of the S-phase cells are even more markedly enhanced. In addition to directly modulating the cell cycle, thermotherapy can also affect the radiosensitivity in another way. Hyperthermia leads to the denaturation of some proteins, including a variety of DNA-repair enzymes, resulting in irreversible damage [19].Fig. 5


Photo-thermal effect enhances the efficiency of radiotherapy using Arg-Gly-Asp peptides-conjugated gold nanorods that target αvβ3 in melanoma cancer cells.

Li P, Shi YW, Li BX, Xu WC, Shi ZL, Zhou C, Fu S - J Nanobiotechnology (2015)

The apoptosis of the A375 cells after NIR or/and RGD-GNRs treatment. A375 cells were treated with RGD-GNRs or/and irradiation for 1 h prior to irradiation (6 mV X-rays with a dose of 4 Gy). The cells were stained with Annexin V and propidium iodide, and apoptosis was analyzed by flow cytometry after 24 h of treatment.
© Copyright Policy - OpenAccess
Related In: Results  -  Collection

License 1 - License 2
Show All Figures
getmorefigures.php?uid=PMC4552250&req=5

Fig5: The apoptosis of the A375 cells after NIR or/and RGD-GNRs treatment. A375 cells were treated with RGD-GNRs or/and irradiation for 1 h prior to irradiation (6 mV X-rays with a dose of 4 Gy). The cells were stained with Annexin V and propidium iodide, and apoptosis was analyzed by flow cytometry after 24 h of treatment.
Mentions: Considering that hyperthermia-induced radiosensitization is known to be associated with more sensitive to S phase cells, which are resistant to radiation. It was determined whether a cell cycle rationale could be used to account for the greater efficacy of the photo-thermal treatment and radiotherapy combination (Fig. 5). As shown in Fig. 6, after treatment with RGD-GNRs and NIR for 24 h, there were a significant accumulation of cells in G2/M phase (p = 0.022, compared with other treatment groups) and a drastic decrease of S phase cells (p = 0.014, compared with other treatment groups), No significant difference can be seen in G0/G1 phase cells among the three groups. So hyperthermia may sensitize cells to radiation by regulating the cell cycle. The combination of thermotherapy and radiotherapy increases damage to all phases of tumor cells, but the killing effects of the S-phase cells are even more markedly enhanced. In addition to directly modulating the cell cycle, thermotherapy can also affect the radiosensitivity in another way. Hyperthermia leads to the denaturation of some proteins, including a variety of DNA-repair enzymes, resulting in irreversible damage [19].Fig. 5

Bottom Line: Compared to other treatments, flow cytometric analysis indicated that RT + NIR + RGD-GNRs increased apoptosis (p < 0.001) and decreased the proportion of cells in the more radioresistant S phase (p = 0.014).Treated with NIR + RGD-GNRs, the radiosensitivity was also significantly enhanced (DMFSF2: 1.41).And it would greatly benefit the therapeutic effects of refractory or recurrent malignant cancers.

View Article: PubMed Central - PubMed

Affiliation: Department of Radiation Oncology, Shanghai Proton and Heavy Ion Center, Fudan University Cancer Hospital, Shanghai, People's Republic of China. liping529@gmail.com.

ABSTRACT

Background: Thermotherapy has been known to be one of the most effective adjuvants to radiotherapy (RT) in cancer treatment, but it is not widely implemented clinically due to some limitations, such as, inadequate temperature concentrations to the tumor tissue, nonspecific and non-uniform distribution of heat. So we constructed arginine-glycine-aspartate peptides-conjugated gold nanorods (RGD-GNRs) that target the alpha(v) beta(3) Integrin (αvβ3) and investigate whether the photo-thermal effect of RGD-GNRs by near infrared radiation (NIR) could enhance the efficiency of RT in melanoma cancer cells.

Results: RGD-GNRs could be seen both on the surface of the cell membranes and cytoplasm of A375 cells with high expression of αvβ3. After exposed to 808 nm NIR, RGD-GNRs with various concentrations could be rapidly heated up. Compared to other treatments, flow cytometric analysis indicated that RT + NIR + RGD-GNRs increased apoptosis (p < 0.001) and decreased the proportion of cells in the more radioresistant S phase (p = 0.014). Treated with NIR + RGD-GNRs, the radiosensitivity was also significantly enhanced (DMFSF2: 1.41).

Conclusion: Results of the current study showed the feasibility of using RGD-GNRs for synergetic RT with photo-thermal therapy. And it would greatly benefit the therapeutic effects of refractory or recurrent malignant cancers.

No MeSH data available.


Related in: MedlinePlus