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Photo-thermal effect enhances the efficiency of radiotherapy using Arg-Gly-Asp peptides-conjugated gold nanorods that target αvβ3 in melanoma cancer cells.

Li P, Shi YW, Li BX, Xu WC, Shi ZL, Zhou C, Fu S - J Nanobiotechnology (2015)

Bottom Line: Thermotherapy has been known to be one of the most effective adjuvants to radiotherapy (RT) in cancer treatment, but it is not widely implemented clinically due to some limitations, such as, inadequate temperature concentrations to the tumor tissue, nonspecific and non-uniform distribution of heat.Compared to other treatments, flow cytometric analysis indicated that RT + NIR + RGD-GNRs increased apoptosis (p < 0.001) and decreased the proportion of cells in the more radioresistant S phase (p = 0.014).Treated with NIR + RGD-GNRs, the radiosensitivity was also significantly enhanced (DMFSF2: 1.41).

View Article: PubMed Central - PubMed

Affiliation: Department of Radiation Oncology, Shanghai Proton and Heavy Ion Center, Fudan University Cancer Hospital, Shanghai, People's Republic of China. liping529@gmail.com.

ABSTRACT

Background: Thermotherapy has been known to be one of the most effective adjuvants to radiotherapy (RT) in cancer treatment, but it is not widely implemented clinically due to some limitations, such as, inadequate temperature concentrations to the tumor tissue, nonspecific and non-uniform distribution of heat. So we constructed arginine-glycine-aspartate peptides-conjugated gold nanorods (RGD-GNRs) that target the alpha(v) beta(3) Integrin (αvβ3) and investigate whether the photo-thermal effect of RGD-GNRs by near infrared radiation (NIR) could enhance the efficiency of RT in melanoma cancer cells.

Results: RGD-GNRs could be seen both on the surface of the cell membranes and cytoplasm of A375 cells with high expression of αvβ3. After exposed to 808 nm NIR, RGD-GNRs with various concentrations could be rapidly heated up. Compared to other treatments, flow cytometric analysis indicated that RT + NIR + RGD-GNRs increased apoptosis (p < 0.001) and decreased the proportion of cells in the more radioresistant S phase (p = 0.014). Treated with NIR + RGD-GNRs, the radiosensitivity was also significantly enhanced (DMFSF2: 1.41).

Conclusion: Results of the current study showed the feasibility of using RGD-GNRs for synergetic RT with photo-thermal therapy. And it would greatly benefit the therapeutic effects of refractory or recurrent malignant cancers.

No MeSH data available.


Related in: MedlinePlus

Heating curve of different concentrations of GNRs (a) and RGD-GNRs (b) (0, 0.05, 0.1, 0.5, 1, 2 mg/ml) under 808 nm NIR at a power density of 1 W/cm2.
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Fig3: Heating curve of different concentrations of GNRs (a) and RGD-GNRs (b) (0, 0.05, 0.1, 0.5, 1, 2 mg/ml) under 808 nm NIR at a power density of 1 W/cm2.

Mentions: As is known to all, GNRs exhibit surface plasmon resonance, allowing them to absorb far stronger light in near-infrared area (650–900 nm) [8, 9]. Since it is easy to tune the LSPR wavelengths of GNRs, they can match the center wavelength of NIR laser source in photo-thermal treatment. In this study, the LSPR wavelength of GNR locates at 805 nm. In order to verify the potential of GNRs as the photo-thermal agent, GNRs and RGD-GNRs at different concentrations were exposed to 808 nm NIR at a power density of 1 W/cm2 for 15 min. Figure 3 showed the heating curve of GNRs and RGD-GNRs at different concentrations. It was shown that after exposure to the NIR, each concentration of GNRs or RGD-GNRs rapidly warmed within 1 min. An obvious concentration-dependent temperature increase was observed either in GNRs or in RGD-GNRs, After 15 min of continuous NIR irradiation at 1 W/cm2, the temperature of GNRs or RGD-GNRs group increased about 13 °C when the concentration was 0.05 mg/ml and the growth of temperature was of a concentration-dependent manner. As expected, there was no temperature change at all in the groups that did not treated with RGD-GNRs followed by NIR irradiation. The above results well demonstrate the suitability of RGD-GNRs as efficient radiosensitizers and photo-thermal agents.Fig. 3


Photo-thermal effect enhances the efficiency of radiotherapy using Arg-Gly-Asp peptides-conjugated gold nanorods that target αvβ3 in melanoma cancer cells.

Li P, Shi YW, Li BX, Xu WC, Shi ZL, Zhou C, Fu S - J Nanobiotechnology (2015)

Heating curve of different concentrations of GNRs (a) and RGD-GNRs (b) (0, 0.05, 0.1, 0.5, 1, 2 mg/ml) under 808 nm NIR at a power density of 1 W/cm2.
© Copyright Policy - OpenAccess
Related In: Results  -  Collection

License 1 - License 2
Show All Figures
getmorefigures.php?uid=PMC4552250&req=5

Fig3: Heating curve of different concentrations of GNRs (a) and RGD-GNRs (b) (0, 0.05, 0.1, 0.5, 1, 2 mg/ml) under 808 nm NIR at a power density of 1 W/cm2.
Mentions: As is known to all, GNRs exhibit surface plasmon resonance, allowing them to absorb far stronger light in near-infrared area (650–900 nm) [8, 9]. Since it is easy to tune the LSPR wavelengths of GNRs, they can match the center wavelength of NIR laser source in photo-thermal treatment. In this study, the LSPR wavelength of GNR locates at 805 nm. In order to verify the potential of GNRs as the photo-thermal agent, GNRs and RGD-GNRs at different concentrations were exposed to 808 nm NIR at a power density of 1 W/cm2 for 15 min. Figure 3 showed the heating curve of GNRs and RGD-GNRs at different concentrations. It was shown that after exposure to the NIR, each concentration of GNRs or RGD-GNRs rapidly warmed within 1 min. An obvious concentration-dependent temperature increase was observed either in GNRs or in RGD-GNRs, After 15 min of continuous NIR irradiation at 1 W/cm2, the temperature of GNRs or RGD-GNRs group increased about 13 °C when the concentration was 0.05 mg/ml and the growth of temperature was of a concentration-dependent manner. As expected, there was no temperature change at all in the groups that did not treated with RGD-GNRs followed by NIR irradiation. The above results well demonstrate the suitability of RGD-GNRs as efficient radiosensitizers and photo-thermal agents.Fig. 3

Bottom Line: Thermotherapy has been known to be one of the most effective adjuvants to radiotherapy (RT) in cancer treatment, but it is not widely implemented clinically due to some limitations, such as, inadequate temperature concentrations to the tumor tissue, nonspecific and non-uniform distribution of heat.Compared to other treatments, flow cytometric analysis indicated that RT + NIR + RGD-GNRs increased apoptosis (p < 0.001) and decreased the proportion of cells in the more radioresistant S phase (p = 0.014).Treated with NIR + RGD-GNRs, the radiosensitivity was also significantly enhanced (DMFSF2: 1.41).

View Article: PubMed Central - PubMed

Affiliation: Department of Radiation Oncology, Shanghai Proton and Heavy Ion Center, Fudan University Cancer Hospital, Shanghai, People's Republic of China. liping529@gmail.com.

ABSTRACT

Background: Thermotherapy has been known to be one of the most effective adjuvants to radiotherapy (RT) in cancer treatment, but it is not widely implemented clinically due to some limitations, such as, inadequate temperature concentrations to the tumor tissue, nonspecific and non-uniform distribution of heat. So we constructed arginine-glycine-aspartate peptides-conjugated gold nanorods (RGD-GNRs) that target the alpha(v) beta(3) Integrin (αvβ3) and investigate whether the photo-thermal effect of RGD-GNRs by near infrared radiation (NIR) could enhance the efficiency of RT in melanoma cancer cells.

Results: RGD-GNRs could be seen both on the surface of the cell membranes and cytoplasm of A375 cells with high expression of αvβ3. After exposed to 808 nm NIR, RGD-GNRs with various concentrations could be rapidly heated up. Compared to other treatments, flow cytometric analysis indicated that RT + NIR + RGD-GNRs increased apoptosis (p < 0.001) and decreased the proportion of cells in the more radioresistant S phase (p = 0.014). Treated with NIR + RGD-GNRs, the radiosensitivity was also significantly enhanced (DMFSF2: 1.41).

Conclusion: Results of the current study showed the feasibility of using RGD-GNRs for synergetic RT with photo-thermal therapy. And it would greatly benefit the therapeutic effects of refractory or recurrent malignant cancers.

No MeSH data available.


Related in: MedlinePlus