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Regeneration of rabbit calvarial defects using cells-implanted nano-hydroxyapatite coated silk scaffolds.

Park JY, Yang C, Jung IH, Lim HC, Lee JS, Jung UW, Seo YK, Park JK, Choi SH - Biomater Res (2015)

Bottom Line: New bone formation was observed in the 4 week groups occurring from the periphery of the defects and the silk fibers were closely integrated with the new bone.There was no significant difference in the amount of bone formation between the SS group and the DPSS and PDLSS groups.However, there was no evidence to suggest that the addition of hDPCs and hPDLCs to the current rabbit calvarial defect model can produce an early effect in augmenting osteogenesis.

View Article: PubMed Central - PubMed

Affiliation: Department of Periodontology, Research Institute of Periodontal Regeneration, Yonsei University College of Dentistry, Seoul, South Korea.

ABSTRACT

Background: The aim of this study was to characterize the efficacy of nano-hydroxyapatite-coated silk fibroin constructs as a scaffold for bone tissue engineering and to determine the osteogenic effect of human dental pulp and periodontal ligament derived cells at an early stage of healing in rabbits. 3D silk fibroin constructs were developed and coated using nano-hydroxyapatite crystals. Dental pulp and periodontal ligament cells from extracted human third molars were cultured and seeded onto the silk scaffolds prior to in vivo implantation into 8 male New Zealand White rabbits. Four circular windows 8 mm in diameter were created in the calvarium of each animal. The defects were randomly allocated to the groups; (1) silk scaffold with dental pulp cells (DPSS), (2) silk scaffold with PDL cells (PDLSS), (3) normal saline-soaked silk scaffold (SS), and (4) empty control. The animals were sacrificed 2 (n = 4) or 4 weeks (n = 4) postoperatively. The characteristics of the silk scaffolds before and after cell seeding were analyzed using SEM. Samples were collected for histologic and histomorphometic analysis. ANOVA was used for statistical analysis.

Result: Histologic view of the experimental sites showed well-maintained structure of the silk scaffolds mostly unresorbed at 4 weeks. The SEM observations after cell-seeding revealed attachment of the cells onto silk fibroin with production of extracellular matrix. New bone formation was observed in the 4 week groups occurring from the periphery of the defects and the silk fibers were closely integrated with the new bone. There was no significant difference in the amount of bone formation between the SS group and the DPSS and PDLSS groups.

Conclusion: Within the limitations of this study, silk scaffold is a biocompatible material with potential expediency as an osteoconductive scaffold in bone tissue engineering. However, there was no evidence to suggest that the addition of hDPCs and hPDLCs to the current rabbit calvarial defect model can produce an early effect in augmenting osteogenesis.

No MeSH data available.


Scanning electron microscopy images. A and B shows the cross section and longitudinal section of the silk scaffold respectively prior to the cell culture. Porous, interconnected structure can be observed, ×100 magnification. C and D show the cross section and longitudinal section of the silk scaffold respectively, following the cell culture, ×400 magnification. Arrows represent spreading of the cells with secreted extracellular matrix on the silk fibroin. Arrowheads represent nHA crystals on silk fibroin.
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Fig2: Scanning electron microscopy images. A and B shows the cross section and longitudinal section of the silk scaffold respectively prior to the cell culture. Porous, interconnected structure can be observed, ×100 magnification. C and D show the cross section and longitudinal section of the silk scaffold respectively, following the cell culture, ×400 magnification. Arrows represent spreading of the cells with secreted extracellular matrix on the silk fibroin. Arrowheads represent nHA crystals on silk fibroin.

Mentions: SEM observations of the surface of the nHA-coated silk scaffold revealed a combination of porous architecture of the silk fibroin. A closer examination at a higher magnification revealed organization of the fibrous structures into bundles. Cross-sectional view revealed interconnected porous structure of the silk fibroin construct. Pore sizes varied between 20–80 μm with mean pore diameter of 65 μm (Figure 2A). Longitudinal sections showed an internal structure with fibrous bundles mainly oriented parallel to the scaffold surface.Figure 2


Regeneration of rabbit calvarial defects using cells-implanted nano-hydroxyapatite coated silk scaffolds.

Park JY, Yang C, Jung IH, Lim HC, Lee JS, Jung UW, Seo YK, Park JK, Choi SH - Biomater Res (2015)

Scanning electron microscopy images. A and B shows the cross section and longitudinal section of the silk scaffold respectively prior to the cell culture. Porous, interconnected structure can be observed, ×100 magnification. C and D show the cross section and longitudinal section of the silk scaffold respectively, following the cell culture, ×400 magnification. Arrows represent spreading of the cells with secreted extracellular matrix on the silk fibroin. Arrowheads represent nHA crystals on silk fibroin.
© Copyright Policy - open-access
Related In: Results  -  Collection

License 1 - License 2
Show All Figures
getmorefigures.php?uid=PMC4552159&req=5

Fig2: Scanning electron microscopy images. A and B shows the cross section and longitudinal section of the silk scaffold respectively prior to the cell culture. Porous, interconnected structure can be observed, ×100 magnification. C and D show the cross section and longitudinal section of the silk scaffold respectively, following the cell culture, ×400 magnification. Arrows represent spreading of the cells with secreted extracellular matrix on the silk fibroin. Arrowheads represent nHA crystals on silk fibroin.
Mentions: SEM observations of the surface of the nHA-coated silk scaffold revealed a combination of porous architecture of the silk fibroin. A closer examination at a higher magnification revealed organization of the fibrous structures into bundles. Cross-sectional view revealed interconnected porous structure of the silk fibroin construct. Pore sizes varied between 20–80 μm with mean pore diameter of 65 μm (Figure 2A). Longitudinal sections showed an internal structure with fibrous bundles mainly oriented parallel to the scaffold surface.Figure 2

Bottom Line: New bone formation was observed in the 4 week groups occurring from the periphery of the defects and the silk fibers were closely integrated with the new bone.There was no significant difference in the amount of bone formation between the SS group and the DPSS and PDLSS groups.However, there was no evidence to suggest that the addition of hDPCs and hPDLCs to the current rabbit calvarial defect model can produce an early effect in augmenting osteogenesis.

View Article: PubMed Central - PubMed

Affiliation: Department of Periodontology, Research Institute of Periodontal Regeneration, Yonsei University College of Dentistry, Seoul, South Korea.

ABSTRACT

Background: The aim of this study was to characterize the efficacy of nano-hydroxyapatite-coated silk fibroin constructs as a scaffold for bone tissue engineering and to determine the osteogenic effect of human dental pulp and periodontal ligament derived cells at an early stage of healing in rabbits. 3D silk fibroin constructs were developed and coated using nano-hydroxyapatite crystals. Dental pulp and periodontal ligament cells from extracted human third molars were cultured and seeded onto the silk scaffolds prior to in vivo implantation into 8 male New Zealand White rabbits. Four circular windows 8 mm in diameter were created in the calvarium of each animal. The defects were randomly allocated to the groups; (1) silk scaffold with dental pulp cells (DPSS), (2) silk scaffold with PDL cells (PDLSS), (3) normal saline-soaked silk scaffold (SS), and (4) empty control. The animals were sacrificed 2 (n = 4) or 4 weeks (n = 4) postoperatively. The characteristics of the silk scaffolds before and after cell seeding were analyzed using SEM. Samples were collected for histologic and histomorphometic analysis. ANOVA was used for statistical analysis.

Result: Histologic view of the experimental sites showed well-maintained structure of the silk scaffolds mostly unresorbed at 4 weeks. The SEM observations after cell-seeding revealed attachment of the cells onto silk fibroin with production of extracellular matrix. New bone formation was observed in the 4 week groups occurring from the periphery of the defects and the silk fibers were closely integrated with the new bone. There was no significant difference in the amount of bone formation between the SS group and the DPSS and PDLSS groups.

Conclusion: Within the limitations of this study, silk scaffold is a biocompatible material with potential expediency as an osteoconductive scaffold in bone tissue engineering. However, there was no evidence to suggest that the addition of hDPCs and hPDLCs to the current rabbit calvarial defect model can produce an early effect in augmenting osteogenesis.

No MeSH data available.