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Antioxidative, antimicrobial and cytotoxic properties of Isatis floribunda Boiss. ex Bornm. extracts.

Karakoca K, Ozusaglam MA, Cakmak YS, Erkul SK - EXCLI J (2013)

Bottom Line: The major component in the extract was cholorogenic acid (1980.20 µg/g).The cytotoxic effect of the methanolic root extract was also tested on human breast cell (MCF12A).The results showed that I. floribunda could be used as a natural source in the food and feed industry and clinical and food chemistry, and that the antimicrobial agents could be used against human and fish pathogens.

View Article: PubMed Central - PubMed

Affiliation: Department of Biology, Faculty of Arts and Sciences, Aksaray University, Aksaray, Turkey.

ABSTRACT
The Isatis species has antibacterial, anticancer and antiviral properties and these important endemic plants grow widely in various parts of Anatolian-Turkey. In the present study, the antioxidant activities of Isatis floribunda Boiss. ex Bornm. flower and root extracts were determined with total antioxidant activity, free radical scavenging activity, ferric ion reducing power, and cupric ion reducing antioxidant capacity assay. The total phenolic compounds and flavonoids were also examined for the extracts. The antimicrobial activities of the extracts were investigated by using the disc diffusion and microdilution-broth methods against human and fish pathogen microorganisms. The phenolic composition of I. floribunda root methanolic extract was analyzed by high performance liquid chromatography (HPLC). The major component in the extract was cholorogenic acid (1980.20 µg/g). The cytotoxic effect of the methanolic root extract was also tested on human breast cell (MCF12A). The results showed that I. floribunda could be used as a natural source in the food and feed industry and clinical and food chemistry, and that the antimicrobial agents could be used against human and fish pathogens.

No MeSH data available.


MBC/MFC values of Isatis floribunda flower and root extracts against clinical and food borne human pathogenic microorganisms
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T4: MBC/MFC values of Isatis floribunda flower and root extracts against clinical and food borne human pathogenic microorganisms

Mentions: The disc diffusion test alone is not enough to decide whether the activity type is lethal or static. In order to identify the type of activity, the disc diffusion test should be followed by MIC and MBC/MFC tests (Altuner et al., 2011[1]). However, the tested plant extracts in the study were colored, and the visible growth could not be observed and so MBC/MFC values were determined. The results of the MBC/MFC are shown in Table 4(Tab. 4). The plant extracts of MBC/MFC values varied from 2.82 to 90.00 mg/ml. The lowest MBC value of 5.63 mg/ml was found in flower methanol extract against B. cereus (RSKK 863). Among the flower extracts, n- hexane extract showed the highest MBC value of 90.00 mg/ml against E.coli (ATCC 35218) and S. enteritidis (ATCC 13076). The lowest MBC values of 2.82 mg/ml were found in the DCM root extract against B. cereus (RSKK 863). The root extracts showed the highest MBC value of 22.50 mg/ml against various microorganisms. The results of the MBC show that the root extracts seemed to be more effective than the flower extracts against the test microorganisms used in this study.


Antioxidative, antimicrobial and cytotoxic properties of Isatis floribunda Boiss. ex Bornm. extracts.

Karakoca K, Ozusaglam MA, Cakmak YS, Erkul SK - EXCLI J (2013)

MBC/MFC values of Isatis floribunda flower and root extracts against clinical and food borne human pathogenic microorganisms
© Copyright Policy - open-access
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4552131&req=5

T4: MBC/MFC values of Isatis floribunda flower and root extracts against clinical and food borne human pathogenic microorganisms
Mentions: The disc diffusion test alone is not enough to decide whether the activity type is lethal or static. In order to identify the type of activity, the disc diffusion test should be followed by MIC and MBC/MFC tests (Altuner et al., 2011[1]). However, the tested plant extracts in the study were colored, and the visible growth could not be observed and so MBC/MFC values were determined. The results of the MBC/MFC are shown in Table 4(Tab. 4). The plant extracts of MBC/MFC values varied from 2.82 to 90.00 mg/ml. The lowest MBC value of 5.63 mg/ml was found in flower methanol extract against B. cereus (RSKK 863). Among the flower extracts, n- hexane extract showed the highest MBC value of 90.00 mg/ml against E.coli (ATCC 35218) and S. enteritidis (ATCC 13076). The lowest MBC values of 2.82 mg/ml were found in the DCM root extract against B. cereus (RSKK 863). The root extracts showed the highest MBC value of 22.50 mg/ml against various microorganisms. The results of the MBC show that the root extracts seemed to be more effective than the flower extracts against the test microorganisms used in this study.

Bottom Line: The major component in the extract was cholorogenic acid (1980.20 µg/g).The cytotoxic effect of the methanolic root extract was also tested on human breast cell (MCF12A).The results showed that I. floribunda could be used as a natural source in the food and feed industry and clinical and food chemistry, and that the antimicrobial agents could be used against human and fish pathogens.

View Article: PubMed Central - PubMed

Affiliation: Department of Biology, Faculty of Arts and Sciences, Aksaray University, Aksaray, Turkey.

ABSTRACT
The Isatis species has antibacterial, anticancer and antiviral properties and these important endemic plants grow widely in various parts of Anatolian-Turkey. In the present study, the antioxidant activities of Isatis floribunda Boiss. ex Bornm. flower and root extracts were determined with total antioxidant activity, free radical scavenging activity, ferric ion reducing power, and cupric ion reducing antioxidant capacity assay. The total phenolic compounds and flavonoids were also examined for the extracts. The antimicrobial activities of the extracts were investigated by using the disc diffusion and microdilution-broth methods against human and fish pathogen microorganisms. The phenolic composition of I. floribunda root methanolic extract was analyzed by high performance liquid chromatography (HPLC). The major component in the extract was cholorogenic acid (1980.20 µg/g). The cytotoxic effect of the methanolic root extract was also tested on human breast cell (MCF12A). The results showed that I. floribunda could be used as a natural source in the food and feed industry and clinical and food chemistry, and that the antimicrobial agents could be used against human and fish pathogens.

No MeSH data available.