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Titanium surface modification by using microwave-induced argon plasma in various conditions to enhance osteoblast biocompatibility.

Seon GM, Seo HJ, Kwon SY, Lee MH, Kwon BJ, Kim MS, Koo MA, Park BJ, Park JC - Biomater Res (2015)

Bottom Line: Microwave-induced argon plasma treatment changed the titanium surface to be very hydrophilic especially on the 5 s short treatment and 30 s, 90 s long treatment samples that detected by contact angle measurement.MC3T3-E1 attachment and proliferation assay significantly increased in 5 s at short treatment, 30 s, and 90 s at long treatment after 5 days incubation.Result indicated that microwave-induce argon plasma treatment would be an effective method to modify titanium surface for enhancing cell-material interactions.

View Article: PubMed Central - PubMed

Affiliation: Cellbiocontrol Laboratory, Department of Medical Engineering, Yonsei University College of Medicine, 134 Shinchon-dong, Seodaemun-gu, Seoul, 120-752 Republic of Korea ; Brain Korea 21 PLUS Project for Medical Science, Yonsei University College of Medicine, 134 Shinchon-dong, Seodaemun-gu, Seoul, 120-752 Republic of Korea.

ABSTRACT

Background: Titanium is a well proven implantable material especially for osseointegratable implants by its biocompatibility and anti-corrosive surface properties. Surface characteristics of the implant play an important role for the evolution of bone tissue of the recipient site. Among the various surface modification methods, plasma treatment is one of the promising methods for enhance biocompatibility. We made microwave-induced argon plasma at atmospheric pressure to improve in titanium surface biocompatibility.

Results: Various states of emission spectra from excited species-argon, nitrogen atoms and oxygen atoms were observed. The electron energy band structures are the unique characteristics of atoms and functional groups. Microwave-induced argon plasma treatment changed the titanium surface to be very hydrophilic especially on the 5 s short treatment and 30 s, 90 s long treatment samples that detected by contact angle measurement. MC3T3-E1 attachment and proliferation assay significantly increased in 5 s at short treatment, 30 s, and 90 s at long treatment after 5 days incubation.

Conclusions: Result indicated that microwave-induce argon plasma treatment would be an effective method to modify titanium surface for enhancing cell-material interactions.

No MeSH data available.


Related in: MedlinePlus

Long treatment condition; Change of morphology of attached MC3T3-E1 cells on plasma treated titanium. (a) Untreated sample. (b) 10 s treated sample. (c) 30 s treated sample. (d) 90 s treated sample. (e) Relative morphometric analysis of attached MC3T3-E1 cells on plasma treated titanium
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Fig5: Long treatment condition; Change of morphology of attached MC3T3-E1 cells on plasma treated titanium. (a) Untreated sample. (b) 10 s treated sample. (c) 30 s treated sample. (d) 90 s treated sample. (e) Relative morphometric analysis of attached MC3T3-E1 cells on plasma treated titanium

Mentions: Fluorescence images of cells on the titanium disks visualizing the plasma membrane by Texas Red maleimide staining have shown that, the attached cells on plasma treated samples were increased in population and also obviously enlarged. Cells adhering to non-treated disks were smaller and round-shaped, while cells on microwave induced plasma treated disks occupied larger attachment area, well-spread elongated morphology with formed lamellipodia-like projections (Figs. 5 and 6). Population of cells also increased after plasma treatment. This result means that the ratio of initially attached cells increased after plasma treatment.Fig. 5


Titanium surface modification by using microwave-induced argon plasma in various conditions to enhance osteoblast biocompatibility.

Seon GM, Seo HJ, Kwon SY, Lee MH, Kwon BJ, Kim MS, Koo MA, Park BJ, Park JC - Biomater Res (2015)

Long treatment condition; Change of morphology of attached MC3T3-E1 cells on plasma treated titanium. (a) Untreated sample. (b) 10 s treated sample. (c) 30 s treated sample. (d) 90 s treated sample. (e) Relative morphometric analysis of attached MC3T3-E1 cells on plasma treated titanium
© Copyright Policy - open-access
Related In: Results  -  Collection

License 1 - License 2
Show All Figures
getmorefigures.php?uid=PMC4552097&req=5

Fig5: Long treatment condition; Change of morphology of attached MC3T3-E1 cells on plasma treated titanium. (a) Untreated sample. (b) 10 s treated sample. (c) 30 s treated sample. (d) 90 s treated sample. (e) Relative morphometric analysis of attached MC3T3-E1 cells on plasma treated titanium
Mentions: Fluorescence images of cells on the titanium disks visualizing the plasma membrane by Texas Red maleimide staining have shown that, the attached cells on plasma treated samples were increased in population and also obviously enlarged. Cells adhering to non-treated disks were smaller and round-shaped, while cells on microwave induced plasma treated disks occupied larger attachment area, well-spread elongated morphology with formed lamellipodia-like projections (Figs. 5 and 6). Population of cells also increased after plasma treatment. This result means that the ratio of initially attached cells increased after plasma treatment.Fig. 5

Bottom Line: Microwave-induced argon plasma treatment changed the titanium surface to be very hydrophilic especially on the 5 s short treatment and 30 s, 90 s long treatment samples that detected by contact angle measurement.MC3T3-E1 attachment and proliferation assay significantly increased in 5 s at short treatment, 30 s, and 90 s at long treatment after 5 days incubation.Result indicated that microwave-induce argon plasma treatment would be an effective method to modify titanium surface for enhancing cell-material interactions.

View Article: PubMed Central - PubMed

Affiliation: Cellbiocontrol Laboratory, Department of Medical Engineering, Yonsei University College of Medicine, 134 Shinchon-dong, Seodaemun-gu, Seoul, 120-752 Republic of Korea ; Brain Korea 21 PLUS Project for Medical Science, Yonsei University College of Medicine, 134 Shinchon-dong, Seodaemun-gu, Seoul, 120-752 Republic of Korea.

ABSTRACT

Background: Titanium is a well proven implantable material especially for osseointegratable implants by its biocompatibility and anti-corrosive surface properties. Surface characteristics of the implant play an important role for the evolution of bone tissue of the recipient site. Among the various surface modification methods, plasma treatment is one of the promising methods for enhance biocompatibility. We made microwave-induced argon plasma at atmospheric pressure to improve in titanium surface biocompatibility.

Results: Various states of emission spectra from excited species-argon, nitrogen atoms and oxygen atoms were observed. The electron energy band structures are the unique characteristics of atoms and functional groups. Microwave-induced argon plasma treatment changed the titanium surface to be very hydrophilic especially on the 5 s short treatment and 30 s, 90 s long treatment samples that detected by contact angle measurement. MC3T3-E1 attachment and proliferation assay significantly increased in 5 s at short treatment, 30 s, and 90 s at long treatment after 5 days incubation.

Conclusions: Result indicated that microwave-induce argon plasma treatment would be an effective method to modify titanium surface for enhancing cell-material interactions.

No MeSH data available.


Related in: MedlinePlus