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Titanium surface modification by using microwave-induced argon plasma in various conditions to enhance osteoblast biocompatibility.

Seon GM, Seo HJ, Kwon SY, Lee MH, Kwon BJ, Kim MS, Koo MA, Park BJ, Park JC - Biomater Res (2015)

Bottom Line: Microwave-induced argon plasma treatment changed the titanium surface to be very hydrophilic especially on the 5 s short treatment and 30 s, 90 s long treatment samples that detected by contact angle measurement.MC3T3-E1 attachment and proliferation assay significantly increased in 5 s at short treatment, 30 s, and 90 s at long treatment after 5 days incubation.Result indicated that microwave-induce argon plasma treatment would be an effective method to modify titanium surface for enhancing cell-material interactions.

View Article: PubMed Central - PubMed

Affiliation: Cellbiocontrol Laboratory, Department of Medical Engineering, Yonsei University College of Medicine, 134 Shinchon-dong, Seodaemun-gu, Seoul, 120-752 Republic of Korea ; Brain Korea 21 PLUS Project for Medical Science, Yonsei University College of Medicine, 134 Shinchon-dong, Seodaemun-gu, Seoul, 120-752 Republic of Korea.

ABSTRACT

Background: Titanium is a well proven implantable material especially for osseointegratable implants by its biocompatibility and anti-corrosive surface properties. Surface characteristics of the implant play an important role for the evolution of bone tissue of the recipient site. Among the various surface modification methods, plasma treatment is one of the promising methods for enhance biocompatibility. We made microwave-induced argon plasma at atmospheric pressure to improve in titanium surface biocompatibility.

Results: Various states of emission spectra from excited species-argon, nitrogen atoms and oxygen atoms were observed. The electron energy band structures are the unique characteristics of atoms and functional groups. Microwave-induced argon plasma treatment changed the titanium surface to be very hydrophilic especially on the 5 s short treatment and 30 s, 90 s long treatment samples that detected by contact angle measurement. MC3T3-E1 attachment and proliferation assay significantly increased in 5 s at short treatment, 30 s, and 90 s at long treatment after 5 days incubation.

Conclusions: Result indicated that microwave-induce argon plasma treatment would be an effective method to modify titanium surface for enhancing cell-material interactions.

No MeSH data available.


Related in: MedlinePlus

MC3T3-E1 cell proliferation on plasma treated titanium surface by plasma treated time. (a) Long treatment condition; MC3T3-E1 proliferation test on titanium surface by MTT assay (b) Short treatment condition; MC3T3-E1 proliferation test on titanium surface by MTT assay
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Fig4: MC3T3-E1 cell proliferation on plasma treated titanium surface by plasma treated time. (a) Long treatment condition; MC3T3-E1 proliferation test on titanium surface by MTT assay (b) Short treatment condition; MC3T3-E1 proliferation test on titanium surface by MTT assay

Mentions: Enhanced proliferation patterns of MC3T3-E1 cultured for 1, 3 and 5 days on the plasma treated titanium were plotted in Fig. 4. After 3 days incubation, the number of cells at ‘long treatment’ was significantly increased after 90 s plasma treatment and the number of cells after 30 s treatment was also significantly increased after 5 days incubation. Moreover, in the short treatment test, cell proliferation was increased just in 5 s treatment time.Fig. 4


Titanium surface modification by using microwave-induced argon plasma in various conditions to enhance osteoblast biocompatibility.

Seon GM, Seo HJ, Kwon SY, Lee MH, Kwon BJ, Kim MS, Koo MA, Park BJ, Park JC - Biomater Res (2015)

MC3T3-E1 cell proliferation on plasma treated titanium surface by plasma treated time. (a) Long treatment condition; MC3T3-E1 proliferation test on titanium surface by MTT assay (b) Short treatment condition; MC3T3-E1 proliferation test on titanium surface by MTT assay
© Copyright Policy - open-access
Related In: Results  -  Collection

License 1 - License 2
Show All Figures
getmorefigures.php?uid=PMC4552097&req=5

Fig4: MC3T3-E1 cell proliferation on plasma treated titanium surface by plasma treated time. (a) Long treatment condition; MC3T3-E1 proliferation test on titanium surface by MTT assay (b) Short treatment condition; MC3T3-E1 proliferation test on titanium surface by MTT assay
Mentions: Enhanced proliferation patterns of MC3T3-E1 cultured for 1, 3 and 5 days on the plasma treated titanium were plotted in Fig. 4. After 3 days incubation, the number of cells at ‘long treatment’ was significantly increased after 90 s plasma treatment and the number of cells after 30 s treatment was also significantly increased after 5 days incubation. Moreover, in the short treatment test, cell proliferation was increased just in 5 s treatment time.Fig. 4

Bottom Line: Microwave-induced argon plasma treatment changed the titanium surface to be very hydrophilic especially on the 5 s short treatment and 30 s, 90 s long treatment samples that detected by contact angle measurement.MC3T3-E1 attachment and proliferation assay significantly increased in 5 s at short treatment, 30 s, and 90 s at long treatment after 5 days incubation.Result indicated that microwave-induce argon plasma treatment would be an effective method to modify titanium surface for enhancing cell-material interactions.

View Article: PubMed Central - PubMed

Affiliation: Cellbiocontrol Laboratory, Department of Medical Engineering, Yonsei University College of Medicine, 134 Shinchon-dong, Seodaemun-gu, Seoul, 120-752 Republic of Korea ; Brain Korea 21 PLUS Project for Medical Science, Yonsei University College of Medicine, 134 Shinchon-dong, Seodaemun-gu, Seoul, 120-752 Republic of Korea.

ABSTRACT

Background: Titanium is a well proven implantable material especially for osseointegratable implants by its biocompatibility and anti-corrosive surface properties. Surface characteristics of the implant play an important role for the evolution of bone tissue of the recipient site. Among the various surface modification methods, plasma treatment is one of the promising methods for enhance biocompatibility. We made microwave-induced argon plasma at atmospheric pressure to improve in titanium surface biocompatibility.

Results: Various states of emission spectra from excited species-argon, nitrogen atoms and oxygen atoms were observed. The electron energy band structures are the unique characteristics of atoms and functional groups. Microwave-induced argon plasma treatment changed the titanium surface to be very hydrophilic especially on the 5 s short treatment and 30 s, 90 s long treatment samples that detected by contact angle measurement. MC3T3-E1 attachment and proliferation assay significantly increased in 5 s at short treatment, 30 s, and 90 s at long treatment after 5 days incubation.

Conclusions: Result indicated that microwave-induce argon plasma treatment would be an effective method to modify titanium surface for enhancing cell-material interactions.

No MeSH data available.


Related in: MedlinePlus