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Repetitive Glucose Spikes Accelerate Atherosclerotic Lesion Formation in C57BL/6 Mice.

Shuto Y, Asai A, Nagao M, Sugihara H, Oikawa S - PLoS ONE (2015)

Bottom Line: Atherosclerotic lesion formation in the aortic sinus was quantitatively analyzed in serial cross-sections by oil red O staining.No significant differences in glucose tolerance, insulin sensitivity, and plasma lipid profiles were observed after the 20-week repetitive administration between the 2 groups.These results indicate that glucose spikes can accelerate atherosclerotic lesion formation, with little influence on other metabolic disorders.

View Article: PubMed Central - PubMed

Affiliation: Department of Endocrinology, Diabetes and Metabolism, Graduate School of Medicine, Nippon Medical School, Tokyo, Japan.

ABSTRACT

Background: A number of epidemiological studies demonstrated that postprandial hyperglycemia is a risk factor for cardiovascular disease in individuals with impaired glucose tolerance. Although several laboratory studies have addressed the plausible causal role of postprandial acute hyperglycemia (glucose spikes) in the development of atherosclerosis, there is little convincing evidence in vivo whether the atherosclerotic lesion formation can be accelerated solely by glucose spikes. Here, we assessed the effect of repetitive glucose spikes on atherosclerotic lesion formation in mice.

Methods: Female C57BL/6 mice were fed an atherogenic diet from 8 to 28 weeks of age. During the atherogenic diet feeding period, the mice orally received a glucose solution (50 mg glucose/mouse; G group) or water (W group) twice daily, 6 days a week. Atherosclerotic lesion formation in the aortic sinus was quantitatively analyzed in serial cross-sections by oil red O staining.

Results: G group mice showed transient increases in blood glucose level (~5 mmol/L above W group), and the levels returned to levels similar to those in W group mice within 60 min. No significant differences in glucose tolerance, insulin sensitivity, and plasma lipid profiles were observed after the 20-week repetitive administration between the 2 groups. G group mice showed an approximately 4-fold greater atherosclerotic lesion size in the aortic sinus than W group mice. Gene expression levels of Cd68 and Icam1 in the thoracic aorta were higher in G group mice than in W group mice.

Conclusions: These results indicate that glucose spikes can accelerate atherosclerotic lesion formation, with little influence on other metabolic disorders. Repetitive glucose administration in wild-type mice may serve as a simple and useful approach to better understanding the causal role of glycemic spikes in the development of atherosclerosis.

No MeSH data available.


Related in: MedlinePlus

Atherosclerotic lesions in aortic sinus.(A,B) Representative images of oil red O-stained atherosclerotic lesions in aortic sinus. (C,D) Immunohistochemical staining of macrophages (brown) in the cross-sections adjacent to the oil red O-stained images. (E) Quantitative analysis of the lesion area. Each dot indicates mean size of the oil red O-stained lesion area in each mouse. Bars indicate mean ± SEM of 8 mice for each group.
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pone.0136840.g003: Atherosclerotic lesions in aortic sinus.(A,B) Representative images of oil red O-stained atherosclerotic lesions in aortic sinus. (C,D) Immunohistochemical staining of macrophages (brown) in the cross-sections adjacent to the oil red O-stained images. (E) Quantitative analysis of the lesion area. Each dot indicates mean size of the oil red O-stained lesion area in each mouse. Bars indicate mean ± SEM of 8 mice for each group.

Mentions: After the 20-week administration period, lipid-laden plaque was observed in the intimal area of the aortic sinus of both groups of mice (Fig 3A and 3B). Atherosclerotic lesion formation was confirmed by the immunohistochemical detection of macrophages (Fig 3C and 3D). The lesion size in G group mice was approximately 4-fold greater than that in W group (Fig 3E).


Repetitive Glucose Spikes Accelerate Atherosclerotic Lesion Formation in C57BL/6 Mice.

Shuto Y, Asai A, Nagao M, Sugihara H, Oikawa S - PLoS ONE (2015)

Atherosclerotic lesions in aortic sinus.(A,B) Representative images of oil red O-stained atherosclerotic lesions in aortic sinus. (C,D) Immunohistochemical staining of macrophages (brown) in the cross-sections adjacent to the oil red O-stained images. (E) Quantitative analysis of the lesion area. Each dot indicates mean size of the oil red O-stained lesion area in each mouse. Bars indicate mean ± SEM of 8 mice for each group.
© Copyright Policy
Related In: Results  -  Collection

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Show All Figures
getmorefigures.php?uid=PMC4552094&req=5

pone.0136840.g003: Atherosclerotic lesions in aortic sinus.(A,B) Representative images of oil red O-stained atherosclerotic lesions in aortic sinus. (C,D) Immunohistochemical staining of macrophages (brown) in the cross-sections adjacent to the oil red O-stained images. (E) Quantitative analysis of the lesion area. Each dot indicates mean size of the oil red O-stained lesion area in each mouse. Bars indicate mean ± SEM of 8 mice for each group.
Mentions: After the 20-week administration period, lipid-laden plaque was observed in the intimal area of the aortic sinus of both groups of mice (Fig 3A and 3B). Atherosclerotic lesion formation was confirmed by the immunohistochemical detection of macrophages (Fig 3C and 3D). The lesion size in G group mice was approximately 4-fold greater than that in W group (Fig 3E).

Bottom Line: Atherosclerotic lesion formation in the aortic sinus was quantitatively analyzed in serial cross-sections by oil red O staining.No significant differences in glucose tolerance, insulin sensitivity, and plasma lipid profiles were observed after the 20-week repetitive administration between the 2 groups.These results indicate that glucose spikes can accelerate atherosclerotic lesion formation, with little influence on other metabolic disorders.

View Article: PubMed Central - PubMed

Affiliation: Department of Endocrinology, Diabetes and Metabolism, Graduate School of Medicine, Nippon Medical School, Tokyo, Japan.

ABSTRACT

Background: A number of epidemiological studies demonstrated that postprandial hyperglycemia is a risk factor for cardiovascular disease in individuals with impaired glucose tolerance. Although several laboratory studies have addressed the plausible causal role of postprandial acute hyperglycemia (glucose spikes) in the development of atherosclerosis, there is little convincing evidence in vivo whether the atherosclerotic lesion formation can be accelerated solely by glucose spikes. Here, we assessed the effect of repetitive glucose spikes on atherosclerotic lesion formation in mice.

Methods: Female C57BL/6 mice were fed an atherogenic diet from 8 to 28 weeks of age. During the atherogenic diet feeding period, the mice orally received a glucose solution (50 mg glucose/mouse; G group) or water (W group) twice daily, 6 days a week. Atherosclerotic lesion formation in the aortic sinus was quantitatively analyzed in serial cross-sections by oil red O staining.

Results: G group mice showed transient increases in blood glucose level (~5 mmol/L above W group), and the levels returned to levels similar to those in W group mice within 60 min. No significant differences in glucose tolerance, insulin sensitivity, and plasma lipid profiles were observed after the 20-week repetitive administration between the 2 groups. G group mice showed an approximately 4-fold greater atherosclerotic lesion size in the aortic sinus than W group mice. Gene expression levels of Cd68 and Icam1 in the thoracic aorta were higher in G group mice than in W group mice.

Conclusions: These results indicate that glucose spikes can accelerate atherosclerotic lesion formation, with little influence on other metabolic disorders. Repetitive glucose administration in wild-type mice may serve as a simple and useful approach to better understanding the causal role of glycemic spikes in the development of atherosclerosis.

No MeSH data available.


Related in: MedlinePlus