Limits...
Up-regulation of Serum MiR-130b-3p Level is Associated with Renal Damage in Early Lupus Nephritis.

Wang W, Mou S, Wang L, Zhang M, Shao X, Fang W, Lu R, Qi C, Fan Z, Cao Q, Wang Q, Fang Y, Ni Z - Sci Rep (2015)

Bottom Line: Subsequent study demonstrated that miR-130b-3p in serum of patients with early stage LN were significantly up-regulated when compared with healthy controls.In addition,we have also observed that the expression of a large amount of circulating microRNAs significantly decreased in patients with late stage LN.The opposite effects were observed when transfected with miR-130b-3p inhibitors.

View Article: PubMed Central - PubMed

Affiliation: Department of Nephrology, Renji Hospital, School of Medicine, Shanghai Jiaotong University.

ABSTRACT
Systemic lupus erythematosus (SLE) is a common but severe autoimmune systemic inflammatory disease. Lupus nephritis (LN) is a serious complication of SLE,affecting up to 70% of SLE patients. Circulating microRNAs (miRNA) are emerging as biomarkers for pathological conditions and play significant roles in intercellular communication. In present research, serum samples from healthy control, early and late stage LN patients were used to analyze the expression profile of miRNAs by microarray. Subsequent study demonstrated that miR-130b-3p in serum of patients with early stage LN were significantly up-regulated when compared with healthy controls. In addition,we have also observed that the expression of a large amount of circulating microRNAs significantly decreased in patients with late stage LN. The further analysis found that the expression of serum miR-130b-3p was positively correlated with 24-hour proteinuria and renal chronicity index in patients with early stage LN.Transfection of renal tubular cellline(HK-2)with miR-130b-3p mimics can promote epithelial-mesenchymal transition (EMT). The opposite effects were observed when transfected with miR-130b-3p inhibitors. MiR-130b-3p negatively regulated ERBB2IP expression by directly targeting the 3'-UTR of ERBB2IP The circulating miR-130b-3p might serve as a biomarker and play an important role in renal damage in early stage LN patients.

No MeSH data available.


Related in: MedlinePlus

The association of serum MiR-130b-3p and SLE activity and renal damage.(A) Altered expression of serum miR-130b-3p in early stage LN patients with or without SLE activity. The line indicates the median value per group. Fold regulation is expressed as RQ based on 2−∆∆Ct.; The correlation between serum miR-130b-3p and (B) anti-dsDNA, (C) C3, (D) C4, (E) 24-hour urine proteinor, (F) renal chronicity index in early stage LN.
© Copyright Policy - open-access
Related In: Results  -  Collection

License
getmorefigures.php?uid=PMC4551961&req=5

f3: The association of serum MiR-130b-3p and SLE activity and renal damage.(A) Altered expression of serum miR-130b-3p in early stage LN patients with or without SLE activity. The line indicates the median value per group. Fold regulation is expressed as RQ based on 2−∆∆Ct.; The correlation between serum miR-130b-3p and (B) anti-dsDNA, (C) C3, (D) C4, (E) 24-hour urine proteinor, (F) renal chronicity index in early stage LN.

Mentions: To determine whether serum miR-130b-3p correlates with SLE activity, early stage LN patients were divided into inactive and active groups based on the SLE disease activity index (SLEDAI). There are 22 patients whose SLEDAI score <9 belong to inactive group and 18 patients whose SLEDAI ≥ 9 to active group23. The anti-double stranded DNA (anti- dsDNA), complement 3(C3), complement 4(C4), 24-hours urinary proteinuria, sedimentation rate (ESR) were significantly different between active and inactive group (Table 2). The miR-130b-3p expression was significantly upregulated both in inactive and active patients as compared to healthy controls [IQR 15.2 (10.1, 36.4) vs9.6 (4.8, 17.4), P < 0.05; and 20.4 (8.0, 43.8) vs9.6 (4.8, 17.4), P < 0.05]. In spite of a higher median, there were no statistical significance found between active group and inactive group (P > 0.05) (Fig. 3A). The association of serum miR-130b-3p and various disease activity parameters were found not significant correlated such as anti-dsDNA (Fig. 3B), C3 (Fig. 3C), C4 (Fig. 3D), etc(details in Table 3). The data showed that the expression level of serum miR-130b-3p may not be affected by SLE disease activity.


Up-regulation of Serum MiR-130b-3p Level is Associated with Renal Damage in Early Lupus Nephritis.

Wang W, Mou S, Wang L, Zhang M, Shao X, Fang W, Lu R, Qi C, Fan Z, Cao Q, Wang Q, Fang Y, Ni Z - Sci Rep (2015)

The association of serum MiR-130b-3p and SLE activity and renal damage.(A) Altered expression of serum miR-130b-3p in early stage LN patients with or without SLE activity. The line indicates the median value per group. Fold regulation is expressed as RQ based on 2−∆∆Ct.; The correlation between serum miR-130b-3p and (B) anti-dsDNA, (C) C3, (D) C4, (E) 24-hour urine proteinor, (F) renal chronicity index in early stage LN.
© Copyright Policy - open-access
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4551961&req=5

f3: The association of serum MiR-130b-3p and SLE activity and renal damage.(A) Altered expression of serum miR-130b-3p in early stage LN patients with or without SLE activity. The line indicates the median value per group. Fold regulation is expressed as RQ based on 2−∆∆Ct.; The correlation between serum miR-130b-3p and (B) anti-dsDNA, (C) C3, (D) C4, (E) 24-hour urine proteinor, (F) renal chronicity index in early stage LN.
Mentions: To determine whether serum miR-130b-3p correlates with SLE activity, early stage LN patients were divided into inactive and active groups based on the SLE disease activity index (SLEDAI). There are 22 patients whose SLEDAI score <9 belong to inactive group and 18 patients whose SLEDAI ≥ 9 to active group23. The anti-double stranded DNA (anti- dsDNA), complement 3(C3), complement 4(C4), 24-hours urinary proteinuria, sedimentation rate (ESR) were significantly different between active and inactive group (Table 2). The miR-130b-3p expression was significantly upregulated both in inactive and active patients as compared to healthy controls [IQR 15.2 (10.1, 36.4) vs9.6 (4.8, 17.4), P < 0.05; and 20.4 (8.0, 43.8) vs9.6 (4.8, 17.4), P < 0.05]. In spite of a higher median, there were no statistical significance found between active group and inactive group (P > 0.05) (Fig. 3A). The association of serum miR-130b-3p and various disease activity parameters were found not significant correlated such as anti-dsDNA (Fig. 3B), C3 (Fig. 3C), C4 (Fig. 3D), etc(details in Table 3). The data showed that the expression level of serum miR-130b-3p may not be affected by SLE disease activity.

Bottom Line: Subsequent study demonstrated that miR-130b-3p in serum of patients with early stage LN were significantly up-regulated when compared with healthy controls.In addition,we have also observed that the expression of a large amount of circulating microRNAs significantly decreased in patients with late stage LN.The opposite effects were observed when transfected with miR-130b-3p inhibitors.

View Article: PubMed Central - PubMed

Affiliation: Department of Nephrology, Renji Hospital, School of Medicine, Shanghai Jiaotong University.

ABSTRACT
Systemic lupus erythematosus (SLE) is a common but severe autoimmune systemic inflammatory disease. Lupus nephritis (LN) is a serious complication of SLE,affecting up to 70% of SLE patients. Circulating microRNAs (miRNA) are emerging as biomarkers for pathological conditions and play significant roles in intercellular communication. In present research, serum samples from healthy control, early and late stage LN patients were used to analyze the expression profile of miRNAs by microarray. Subsequent study demonstrated that miR-130b-3p in serum of patients with early stage LN were significantly up-regulated when compared with healthy controls. In addition,we have also observed that the expression of a large amount of circulating microRNAs significantly decreased in patients with late stage LN. The further analysis found that the expression of serum miR-130b-3p was positively correlated with 24-hour proteinuria and renal chronicity index in patients with early stage LN.Transfection of renal tubular cellline(HK-2)with miR-130b-3p mimics can promote epithelial-mesenchymal transition (EMT). The opposite effects were observed when transfected with miR-130b-3p inhibitors. MiR-130b-3p negatively regulated ERBB2IP expression by directly targeting the 3'-UTR of ERBB2IP The circulating miR-130b-3p might serve as a biomarker and play an important role in renal damage in early stage LN patients.

No MeSH data available.


Related in: MedlinePlus