ΔNp63 intronic miR-944 is implicated in the ΔNp63-mediated induction of epidermal differentiation.
Bottom Line: We found that miR-944 is highly expressed in keratinocytes, in a manner that is concordant with that of ΔNp63 mRNA, but the regulation of miR-944 expression under various conditions did not correspond with that of ΔNp63.Our results indicated that miR-944 biogenesis is dependent on ΔNp63 protein, even though it is generated from ΔNp63 mRNA-independent transcripts.We also demonstrated that miR-944 induces keratin 1 and keratin 10 expression by inhibiting ERK signaling and upregulating p53 expression.
Affiliation: Bioscience Research Division, R&D Unit, AmorePacific Corporation, Yongin-si, Gyeonggi-do 446-729, Republic of Koreaf.Show MeSH
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Mentions: ΔNp63 expression is gradually reduced in the epidermis during differentiation. However, miR-944 expression is not decreased, even though it is the target of ΔNp63. This raised the question of whether miR-944 biogenesis is sustained during differentiation. Interestingly, we found that ΔNp63-binding to the MIR944 promoter is maintained during the differentiation of keratinocytes (Figure 5A); additionally, mutation of the ΔNp63-binding site blocked the differentiation-induced increase in the activation of the MIR944 promoter (Figure 5B).
Affiliation: Bioscience Research Division, R&D Unit, AmorePacific Corporation, Yongin-si, Gyeonggi-do 446-729, Republic of Koreaf.