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Biopsy Diagnosis of Oral Carcinoma by the Combination of Morphological and Spectral Methods Based on Embedded Relay Lens Microscopic Hyperspectral Imaging System.

Ou-Yang M, Hsieh YF, Lee CC - J Med Biol Eng (2015)

Bottom Line: Hence, the proposed cocktail approach is used to determine the effectiveness of the spectral methods in correlating with the sampling conditions.And then we use a combination of effective spectral methods according to the sample conditions for diagnosing a sample.The results demonstrate a sensitivity of 90 ± 4.53 % and a specificity of 87.8 ± 5.21 %.

View Article: PubMed Central - PubMed

Affiliation: Department of Electrical and Computer Engineering, National Chiao-Tung University, 1001 Ta-Hsueh Rd., Hsinchu, Taiwan.

ABSTRACT

Cytopathological examination through biopsy is very important for carcinoma detection. The embedded relay lens microscopic hyperspectral imaging system (ERL-MHIS) provides a morphological image of a biopsy sample and the spectrum of each pixel in the image simultaneously. Based on the ERL-MHIS, this work develops morphological and spectral methods to diagnose oral carcinoma biopsy. In morphological discrimination, the fractal dimension method is applied to differentiate between normal and abnormal tissues. In spectral identification, normal and cancerous cells are distinguished using five methods. However, the spectra of normal and cancerous cells vary with patient. The diagnostic performances of the five methods are thus not ideal. Hence, the proposed cocktail approach is used to determine the effectiveness of the spectral methods in correlating with the sampling conditions. And then we use a combination of effective spectral methods according to the sample conditions for diagnosing a sample. A total of 68 biopsies from 34 patients are analyzed using the ERL-MHIS. The results demonstrate a sensitivity of 90 ± 4.53 % and a specificity of 87.8 ± 5.21 %. Furthermore, in our survey, this system is the first time utilized to study oral carcinoma biopsies.

No MeSH data available.


Related in: MedlinePlus

Comparison of performance of all methods. (a) Mean sensitivity and (b) mean specificity of 29 patients for each method
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Fig5: Comparison of performance of all methods. (a) Mean sensitivity and (b) mean specificity of 29 patients for each method

Mentions: Figure 5 compares the performances of all methods. Because each method was appropriate for only some patients, the standard deviation of each method was large. In contrast, the cocktail approach showed a high mean specificity, high mean sensitivity, and small standard deviation, implying its better correlation with sample data. In addition, the fourteen patients with early stage oral carcinoma were successfully diagnosed with a sensitivity of 90 ± 4.65 % and a specificity of 87.2 ± 5.06 %. Moreover, 10 test samples were utilized to validate the training results (Table 3). Note that the type of sample (normal or cancerous) for the 10 test samples was not revealed to the analyst during their analysis of the samples. The 10 test samples were diagnosed according to the patient’s conditions. The sensitivity and specificity of the 10 test samples were 80.16 ± 4.5 % and 81.74 ± 2.26 %, respectively. Notably, the testing nuclei were chosen from well dyed cells and cells away border. The results can be enhanced by the correct patient’s condition. The concentration and the time of the H&E would be the key conditions.Fig. 5


Biopsy Diagnosis of Oral Carcinoma by the Combination of Morphological and Spectral Methods Based on Embedded Relay Lens Microscopic Hyperspectral Imaging System.

Ou-Yang M, Hsieh YF, Lee CC - J Med Biol Eng (2015)

Comparison of performance of all methods. (a) Mean sensitivity and (b) mean specificity of 29 patients for each method
© Copyright Policy - OpenAccess
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4551557&req=5

Fig5: Comparison of performance of all methods. (a) Mean sensitivity and (b) mean specificity of 29 patients for each method
Mentions: Figure 5 compares the performances of all methods. Because each method was appropriate for only some patients, the standard deviation of each method was large. In contrast, the cocktail approach showed a high mean specificity, high mean sensitivity, and small standard deviation, implying its better correlation with sample data. In addition, the fourteen patients with early stage oral carcinoma were successfully diagnosed with a sensitivity of 90 ± 4.65 % and a specificity of 87.2 ± 5.06 %. Moreover, 10 test samples were utilized to validate the training results (Table 3). Note that the type of sample (normal or cancerous) for the 10 test samples was not revealed to the analyst during their analysis of the samples. The 10 test samples were diagnosed according to the patient’s conditions. The sensitivity and specificity of the 10 test samples were 80.16 ± 4.5 % and 81.74 ± 2.26 %, respectively. Notably, the testing nuclei were chosen from well dyed cells and cells away border. The results can be enhanced by the correct patient’s condition. The concentration and the time of the H&E would be the key conditions.Fig. 5

Bottom Line: Hence, the proposed cocktail approach is used to determine the effectiveness of the spectral methods in correlating with the sampling conditions.And then we use a combination of effective spectral methods according to the sample conditions for diagnosing a sample.The results demonstrate a sensitivity of 90 ± 4.53 % and a specificity of 87.8 ± 5.21 %.

View Article: PubMed Central - PubMed

Affiliation: Department of Electrical and Computer Engineering, National Chiao-Tung University, 1001 Ta-Hsueh Rd., Hsinchu, Taiwan.

ABSTRACT

Cytopathological examination through biopsy is very important for carcinoma detection. The embedded relay lens microscopic hyperspectral imaging system (ERL-MHIS) provides a morphological image of a biopsy sample and the spectrum of each pixel in the image simultaneously. Based on the ERL-MHIS, this work develops morphological and spectral methods to diagnose oral carcinoma biopsy. In morphological discrimination, the fractal dimension method is applied to differentiate between normal and abnormal tissues. In spectral identification, normal and cancerous cells are distinguished using five methods. However, the spectra of normal and cancerous cells vary with patient. The diagnostic performances of the five methods are thus not ideal. Hence, the proposed cocktail approach is used to determine the effectiveness of the spectral methods in correlating with the sampling conditions. And then we use a combination of effective spectral methods according to the sample conditions for diagnosing a sample. A total of 68 biopsies from 34 patients are analyzed using the ERL-MHIS. The results demonstrate a sensitivity of 90 ± 4.53 % and a specificity of 87.8 ± 5.21 %. Furthermore, in our survey, this system is the first time utilized to study oral carcinoma biopsies.

No MeSH data available.


Related in: MedlinePlus