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Capillary electrophoresis applied to DNA: determining and harnessing sequence and structure to advance bioanalyses (2009-2014).

Durney BC, Crihfield CL, Holland LA - Anal Bioanal Chem (2015)

Bottom Line: This review of capillary electrophoresis methods for DNA analyses covers critical advances from 2009 to 2014, referencing 184 citations.Two prevalent gel matrices for gel-facilitated sieving, which are linear polyacrylamide and polydimethylacrylamide, are compared in terms of performance, cost, viscosity, and passivation of electroosmotic flow.Expanding and emerging techniques in the field are also highlighted.

View Article: PubMed Central - PubMed

Affiliation: C. Eugene Bennett Department of Chemistry, West Virginia University, Morgantown, WV, 26506, USA.

ABSTRACT
This review of capillary electrophoresis methods for DNA analyses covers critical advances from 2009 to 2014, referencing 184 citations. Separation mechanisms based on free-zone capillary electrophoresis, Ogston sieving, and reptation are described. Two prevalent gel matrices for gel-facilitated sieving, which are linear polyacrylamide and polydimethylacrylamide, are compared in terms of performance, cost, viscosity, and passivation of electroosmotic flow. The role of capillary electrophoresis in the discovery, design, and characterization of DNA aptamers for molecular recognition is discussed. Expanding and emerging techniques in the field are also highlighted.

No MeSH data available.


Related in: MedlinePlus

Number of publications on capillary electrophoresis separations of DNA in the SciFinder® database
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Fig1: Number of publications on capillary electrophoresis separations of DNA in the SciFinder® database

Mentions: Capillary electrophoresis separations are significant because they provide fast separations of limited sample volumes. Following reports of outstanding separation efficiencies of amines, amino acids, and peptides achieved using a 75-μm-inner-diameter glass capillary [1, 2], the technology was quickly adapted to DNA [3, 4]. The rapid growth and sustained use of capillary electrophoresis for DNA analyses is best illustrated by the number of annual journal publications, which is summarized in Fig. 1. Critical innovations reported early in the method development [5–10] dramatically increased the applicability to sequence and size DNA. Landmark applications include genome sequencing [11], forensic analysis of DNA with commercial systems [12, 13], and lab-on-a-chip [14–17]. In addition to sizing DNA, capillary electrophoresis has played a pivotal role in the generation of DNA aptamers and the quantification of aptamer binding affinity.Fig. 1


Capillary electrophoresis applied to DNA: determining and harnessing sequence and structure to advance bioanalyses (2009-2014).

Durney BC, Crihfield CL, Holland LA - Anal Bioanal Chem (2015)

Number of publications on capillary electrophoresis separations of DNA in the SciFinder® database
© Copyright Policy - OpenAccess
Related In: Results  -  Collection

Show All Figures
getmorefigures.php?uid=PMC4551542&req=5

Fig1: Number of publications on capillary electrophoresis separations of DNA in the SciFinder® database
Mentions: Capillary electrophoresis separations are significant because they provide fast separations of limited sample volumes. Following reports of outstanding separation efficiencies of amines, amino acids, and peptides achieved using a 75-μm-inner-diameter glass capillary [1, 2], the technology was quickly adapted to DNA [3, 4]. The rapid growth and sustained use of capillary electrophoresis for DNA analyses is best illustrated by the number of annual journal publications, which is summarized in Fig. 1. Critical innovations reported early in the method development [5–10] dramatically increased the applicability to sequence and size DNA. Landmark applications include genome sequencing [11], forensic analysis of DNA with commercial systems [12, 13], and lab-on-a-chip [14–17]. In addition to sizing DNA, capillary electrophoresis has played a pivotal role in the generation of DNA aptamers and the quantification of aptamer binding affinity.Fig. 1

Bottom Line: This review of capillary electrophoresis methods for DNA analyses covers critical advances from 2009 to 2014, referencing 184 citations.Two prevalent gel matrices for gel-facilitated sieving, which are linear polyacrylamide and polydimethylacrylamide, are compared in terms of performance, cost, viscosity, and passivation of electroosmotic flow.Expanding and emerging techniques in the field are also highlighted.

View Article: PubMed Central - PubMed

Affiliation: C. Eugene Bennett Department of Chemistry, West Virginia University, Morgantown, WV, 26506, USA.

ABSTRACT
This review of capillary electrophoresis methods for DNA analyses covers critical advances from 2009 to 2014, referencing 184 citations. Separation mechanisms based on free-zone capillary electrophoresis, Ogston sieving, and reptation are described. Two prevalent gel matrices for gel-facilitated sieving, which are linear polyacrylamide and polydimethylacrylamide, are compared in terms of performance, cost, viscosity, and passivation of electroosmotic flow. The role of capillary electrophoresis in the discovery, design, and characterization of DNA aptamers for molecular recognition is discussed. Expanding and emerging techniques in the field are also highlighted.

No MeSH data available.


Related in: MedlinePlus