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Prosystemin Overexpression in Tomato Enhances Resistance to Different Biotic Stresses by Activating Genes of Multiple Signaling Pathways.

Coppola M, Corrado G, Coppola V, Cascone P, Martinelli R, Digilio MC, Pennacchio F, Rao R - Plant Mol. Biol. Rep. (2015)

Bottom Line: These alterations present a degree of overlap with the response programs that are classically associated to pathogen defense or abiotic stress protection, implying that end products of the systemin signaling pathway may be more diverse than expected.Our work demonstrated that in tomato the modulation of a single gene is sufficient to provide a wide resistance against stress by boosting endogenous defense pathways.Overall, the data provided evidence that the systemin peptide might serve as DAMP signal in tomato, acting as a broad indicator of tissue integrity.

View Article: PubMed Central - PubMed

Affiliation: Dipartimento di Agraria, Università degli Studi di Napoli Federico II, 80055 Portici, NA Italy.

ABSTRACT

Systemin is a signal peptide that promotes the response to wounding and herbivore attack in tomato. This 18-amino acid peptide is released from a larger precursor, prosystemin. To study the role of systemin as a modulator of defense signaling, we generated tomato (Solanum lycopersicum) transgenic plants that overexpress the prosystemin cDNA. We carried out a transcriptomic analysis comparing two different transgenic events with the untransformed control. The Gene Ontology categories of the 503 differentially expressed genes indicated that several biological functions were affected. Systemin promotes the expression of an array of defense genes that are dependent on different signaling pathways and it downregulates genes connected with carbon fixation and carbohydrate metabolism. These alterations present a degree of overlap with the response programs that are classically associated to pathogen defense or abiotic stress protection, implying that end products of the systemin signaling pathway may be more diverse than expected. We show also that the observed transcriptional modifications have a relevant functional outcome, since transgenic lines were more resistant against very different biotic stressors such as aphids (Macrosiphum euphorbiae), phytopathogenic fungi (Botrytis cinerea and Alternaria alternata) and phytophagous larvae (Spodoptera littoralis). Our work demonstrated that in tomato the modulation of a single gene is sufficient to provide a wide resistance against stress by boosting endogenous defense pathways. Overall, the data provided evidence that the systemin peptide might serve as DAMP signal in tomato, acting as a broad indicator of tissue integrity.

No MeSH data available.


Related in: MedlinePlus

Expression analysis of selected genes in Red Setter leaves following aphid infestation. Relative quantification (RQ) of gene expression by real-time RT-PCR of selected differentially expressed genes. Pin I proteinase inhibitor I, Pin II proteinase inhibitor II, KPI Kunitz-type proteinase inhibitor family protein, LAP leucine amino peptidase, TD threonine deaminase, PR1 pathogenesis-related protein 1. The 2−ΔΔCt values were significantly different between treated and control plants (*p < 0.05; **p < 0.01; Student’s t test)
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Fig7: Expression analysis of selected genes in Red Setter leaves following aphid infestation. Relative quantification (RQ) of gene expression by real-time RT-PCR of selected differentially expressed genes. Pin I proteinase inhibitor I, Pin II proteinase inhibitor II, KPI Kunitz-type proteinase inhibitor family protein, LAP leucine amino peptidase, TD threonine deaminase, PR1 pathogenesis-related protein 1. The 2−ΔΔCt values were significantly different between treated and control plants (*p < 0.05; **p < 0.01; Student’s t test)

Mentions: The possible overlap between the pathways activated by prosystemin and by the aphid feeding was investigated analyzing the expression level, following aphid infestation, of genes selected from the microarray study. Leaves were harvested 96 h post infestation, when aphids have established a feeding site. The data indicated that the selected genes (Pin I, Pin II, KPI, LAP, TD, PR1, and WRKY40) were differentially expressed following aphid infestation (Fig. 7). An interesting difference between the response to aphids and to prosystemin was related to PR1 expression, a protein typically produced in plants in the event of a pathogen attack. PR1 was downregulated in the RSYS plant but induced by aphid feeding. The latter is, for instance, consistent with data in Arabidopsis thaliana seedlings (Moran and Thompson 2001) and indicated that tomato response to aphids is complex and shares components with both fungal pathogens and herbivores (Coppola et al. 2013; Smith and Boyko 2007; Thompson and Goggin 2006).Fig. 7


Prosystemin Overexpression in Tomato Enhances Resistance to Different Biotic Stresses by Activating Genes of Multiple Signaling Pathways.

Coppola M, Corrado G, Coppola V, Cascone P, Martinelli R, Digilio MC, Pennacchio F, Rao R - Plant Mol. Biol. Rep. (2015)

Expression analysis of selected genes in Red Setter leaves following aphid infestation. Relative quantification (RQ) of gene expression by real-time RT-PCR of selected differentially expressed genes. Pin I proteinase inhibitor I, Pin II proteinase inhibitor II, KPI Kunitz-type proteinase inhibitor family protein, LAP leucine amino peptidase, TD threonine deaminase, PR1 pathogenesis-related protein 1. The 2−ΔΔCt values were significantly different between treated and control plants (*p < 0.05; **p < 0.01; Student’s t test)
© Copyright Policy - OpenAccess
Related In: Results  -  Collection

Show All Figures
getmorefigures.php?uid=PMC4551541&req=5

Fig7: Expression analysis of selected genes in Red Setter leaves following aphid infestation. Relative quantification (RQ) of gene expression by real-time RT-PCR of selected differentially expressed genes. Pin I proteinase inhibitor I, Pin II proteinase inhibitor II, KPI Kunitz-type proteinase inhibitor family protein, LAP leucine amino peptidase, TD threonine deaminase, PR1 pathogenesis-related protein 1. The 2−ΔΔCt values were significantly different between treated and control plants (*p < 0.05; **p < 0.01; Student’s t test)
Mentions: The possible overlap between the pathways activated by prosystemin and by the aphid feeding was investigated analyzing the expression level, following aphid infestation, of genes selected from the microarray study. Leaves were harvested 96 h post infestation, when aphids have established a feeding site. The data indicated that the selected genes (Pin I, Pin II, KPI, LAP, TD, PR1, and WRKY40) were differentially expressed following aphid infestation (Fig. 7). An interesting difference between the response to aphids and to prosystemin was related to PR1 expression, a protein typically produced in plants in the event of a pathogen attack. PR1 was downregulated in the RSYS plant but induced by aphid feeding. The latter is, for instance, consistent with data in Arabidopsis thaliana seedlings (Moran and Thompson 2001) and indicated that tomato response to aphids is complex and shares components with both fungal pathogens and herbivores (Coppola et al. 2013; Smith and Boyko 2007; Thompson and Goggin 2006).Fig. 7

Bottom Line: These alterations present a degree of overlap with the response programs that are classically associated to pathogen defense or abiotic stress protection, implying that end products of the systemin signaling pathway may be more diverse than expected.Our work demonstrated that in tomato the modulation of a single gene is sufficient to provide a wide resistance against stress by boosting endogenous defense pathways.Overall, the data provided evidence that the systemin peptide might serve as DAMP signal in tomato, acting as a broad indicator of tissue integrity.

View Article: PubMed Central - PubMed

Affiliation: Dipartimento di Agraria, Università degli Studi di Napoli Federico II, 80055 Portici, NA Italy.

ABSTRACT

Systemin is a signal peptide that promotes the response to wounding and herbivore attack in tomato. This 18-amino acid peptide is released from a larger precursor, prosystemin. To study the role of systemin as a modulator of defense signaling, we generated tomato (Solanum lycopersicum) transgenic plants that overexpress the prosystemin cDNA. We carried out a transcriptomic analysis comparing two different transgenic events with the untransformed control. The Gene Ontology categories of the 503 differentially expressed genes indicated that several biological functions were affected. Systemin promotes the expression of an array of defense genes that are dependent on different signaling pathways and it downregulates genes connected with carbon fixation and carbohydrate metabolism. These alterations present a degree of overlap with the response programs that are classically associated to pathogen defense or abiotic stress protection, implying that end products of the systemin signaling pathway may be more diverse than expected. We show also that the observed transcriptional modifications have a relevant functional outcome, since transgenic lines were more resistant against very different biotic stressors such as aphids (Macrosiphum euphorbiae), phytopathogenic fungi (Botrytis cinerea and Alternaria alternata) and phytophagous larvae (Spodoptera littoralis). Our work demonstrated that in tomato the modulation of a single gene is sufficient to provide a wide resistance against stress by boosting endogenous defense pathways. Overall, the data provided evidence that the systemin peptide might serve as DAMP signal in tomato, acting as a broad indicator of tissue integrity.

No MeSH data available.


Related in: MedlinePlus